Abstract
Mutagenesis has been the foundation of genetics in most model organisms, from prokaryotes to the fruit flies. The simplicity of mutagenizing and propagating these creatures is critical for projects designed to isolate mutations affecting most any imaginable biological process. Not only has mutagenesis been exploited for the identification of phenotypic mutants, but also for the derivation of chromosomal aberrations, such as deletions and inversions, that are powerful genetic tools for many sorts of experiments. The laboratory mouse, which serves as the most important and powerful animal model for human genetics and disease, is only recently being widely exploited by classical phenotype-driven mutagenesis. The limitation has been (and still remains) related to the biology and life cycle of the mouse, which renders large-scale mutagenesis projects rather cumbersome and expensive. Is classical chemical mutagenesis really worthwhile, given the powerful germline manipulation technologies available for mic e Are there new technologies that will render classical mutagenesis obsolete In this review, I shall discuss the tools of mutagenesis that are available for mice, and consider emerging and potential future technologies that will ultimately allow us to investigate, in an unprecedented way, the in vivo function of all mammalian genes.
Keywords: Mutagenesis, CDNA, EST Expressed Sequence Tag
Current Genomics
Title: Mutagenesis in Mice Modern Times
Volume: 1 Issue: 3
Author(s): John C. Schimenti
Affiliation:
Keywords: Mutagenesis, CDNA, EST Expressed Sequence Tag
Abstract: Mutagenesis has been the foundation of genetics in most model organisms, from prokaryotes to the fruit flies. The simplicity of mutagenizing and propagating these creatures is critical for projects designed to isolate mutations affecting most any imaginable biological process. Not only has mutagenesis been exploited for the identification of phenotypic mutants, but also for the derivation of chromosomal aberrations, such as deletions and inversions, that are powerful genetic tools for many sorts of experiments. The laboratory mouse, which serves as the most important and powerful animal model for human genetics and disease, is only recently being widely exploited by classical phenotype-driven mutagenesis. The limitation has been (and still remains) related to the biology and life cycle of the mouse, which renders large-scale mutagenesis projects rather cumbersome and expensive. Is classical chemical mutagenesis really worthwhile, given the powerful germline manipulation technologies available for mic e Are there new technologies that will render classical mutagenesis obsolete In this review, I shall discuss the tools of mutagenesis that are available for mice, and consider emerging and potential future technologies that will ultimately allow us to investigate, in an unprecedented way, the in vivo function of all mammalian genes.
Export Options
About this article
Cite this article as:
Schimenti C. John, Mutagenesis in Mice Modern Times, Current Genomics 2000; 1 (3) . https://dx.doi.org/10.2174/1389202003351409
DOI https://dx.doi.org/10.2174/1389202003351409 |
Print ISSN 1389-2029 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5488 |
Call for Papers in Thematic Issues
Advanced Computational Algorithms and Artificial Intelligence in Clinical Pharmacogenomics
In the era of personalized medicine, understanding the relationship between genetics and drug response is crucial. This issue delves into innovative methodologies, leveraging deep computational analysis and artificial intelligence, to enhance the field of Clinical Pharmacogenomics. The interdisciplinary approach harnesses the power of advanced high-throughput genotyping technologies, sophisticated computational analysis, ...read more
Applications of Single-cell Sequencing Technology in Reproductive Medicine
Single cell sequencing (SCS) technology utilizes individual cells' genetic material to sequence their genome, transcriptome, and epigenetics at the molecular level. It offers insights into cell heterogeneity and enables the study of limited biological materials. Since its recognition as a valuable technique in 2011, single cell sequencing has yielded numerous ...read more
Big Data in Cancer Research
Cancer is a significant threat to human life and health, remaining a highly aggressive killer. It is a leading cause of death worldwide and represents a crucial medical issue for humanity. However, in the past decade, the effectiveness of new synthetic anticancer agents has not matched the current clinical speculation. ...read more
Current Genomics in Cardiovascular Research
Cardiovascular diseases are the main cause of death in the world, in recent years we have had important advances in the interaction between cardiovascular disease and genomics. In this Research Topic, we intend for researchers to present their results with a focus on basic, translational and clinical investigations associated with ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Histone Deacetylation and Motor Neuron Degeneration
CNS & Neurological Disorders - Drug Targets Port-a-Patch and Patchliner: High Fidelity Electrophysiology for Secondary Screening and Safety Pharmacology
Combinatorial Chemistry & High Throughput Screening A Mini-Review on the Synthesis of Pyrazinoindole: Recent Progress and Perspectives
Mini-Reviews in Organic Chemistry ERRATUM:
Current Neuropharmacology The Dual Role of Serotonin in Defense and the Mode of Action of Antidepressants on Generalized Anxiety and Panic Disorders
Central Nervous System Agents in Medicinal Chemistry Structure-Specific Ligand Recognition of Multistranded DNA Structures
Current Topics in Medicinal Chemistry Combination Treatment in Alzheimers Disease: Results of a Randomized, Controlled Trial with Cerebrolysin and Donepezil
Current Alzheimer Research Structural and Functional Characterization of Pathogenic Non- Synonymous Genetic Mutations of Human Insulin-Degrading Enzyme by In Silico Methods
CNS & Neurological Disorders - Drug Targets Trigeminal Neuralgia: Basic and Clinical Aspects
Current Neuropharmacology BACE1 Dependent Neuregulin Processing: Review
Current Alzheimer Research Brain Molecules and Appetite: The Case of Oleoylethanolamide
Central Nervous System Agents in Medicinal Chemistry Multi-particulate Systems: Cutting-edge Technology for Controlled Drug Delivery
Recent Patents on Drug Delivery & Formulation Roles of Glycogen Synthase Kinase 3 in Alzheimer’s Disease
Current Alzheimer Research Sedation in PACU: The Role of Propofol
Current Drug Targets Blocking Striatal Adenosine A2A Receptors: A New Strategy for Basal Ganglia Disorders
Recent Patents on CNS Drug Discovery (Discontinued) Granulomatosis with Polyangiitis (GPA) in a 15-year-old Girl with Facial Acne-like Ulcers: A Case Report
Infectious Disorders - Drug Targets Bioinformatics-Based Characterization of Proteins Related to SARS-CoV- 2 Using the Polarity Index Method<sup>®</sup> (PIM<sup>®</sup>) and Intrinsic Disorder Predisposition
Current Proteomics Targeting Mitochondrial Bioenergetics for Alzheimers Prevention and Treatment
Current Pharmaceutical Design The Effect of On-Demand Caffeine Consumption on Treating Patients with Premature Ejaculation: A Double-Blind Randomized Clinical Trial
Current Pharmaceutical Biotechnology Safety and Side Effects of Cannabidiol, a Cannabis sativa Constituent
Current Drug Safety