Abstract
Technologies to develop and evolve the function of proteins and, in particular, antibodies have developed rapidly since the introduction of phage display. Importantly, it has become possible to identify molecules with binding properties that cannot be found by other means. A range of different approaches to create general libraries that are useful for the selection of such molecules specific for essentially any kind of target have emerged. We herein review some of the most prominent approaches in the field and in particular discuss specific features related to the development of antibody libraries based on single antibody framework scaffolds. This approach not only permits identification of a range of specific binders, but also facilitates further evolution of initially derived molecules into molecules with optimised functions.
Keywords: immune diversity, protein scaffolds, affinity maturation
Combinatorial Chemistry & High Throughput Screening
Title: The Immune Diversity in a Test Tube - Non-Immunised Antibody Libraries and Functional Variability in Defined Protein Scaffolds
Volume: 4 Issue: 5
Author(s): E. Soderlind, R. Carlsson, C. A.K. Borrebaeck and M. Ohlin
Affiliation:
Keywords: immune diversity, protein scaffolds, affinity maturation
Abstract: Technologies to develop and evolve the function of proteins and, in particular, antibodies have developed rapidly since the introduction of phage display. Importantly, it has become possible to identify molecules with binding properties that cannot be found by other means. A range of different approaches to create general libraries that are useful for the selection of such molecules specific for essentially any kind of target have emerged. We herein review some of the most prominent approaches in the field and in particular discuss specific features related to the development of antibody libraries based on single antibody framework scaffolds. This approach not only permits identification of a range of specific binders, but also facilitates further evolution of initially derived molecules into molecules with optimised functions.
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Cite this article as:
Soderlind E., Carlsson R., Borrebaeck A.K. C. and Ohlin M., The Immune Diversity in a Test Tube - Non-Immunised Antibody Libraries and Functional Variability in Defined Protein Scaffolds, Combinatorial Chemistry & High Throughput Screening 2001; 4 (5) . https://dx.doi.org/10.2174/1386207013330968
DOI https://dx.doi.org/10.2174/1386207013330968 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
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