Noninvasive Genotyping of Dopamine Receptor D4 (DRD4) UsingNanograms of DNA From Substance-Dependent Patients

Title: Noninvasive Genotyping of Dopamine Receptor D4 (DRD4) UsingNanograms of DNA From Substance-Dependent Patients

Volume: 9 Issue: 8

Author(s): K. Boor, Z. Ronai, Z. Nemoda, P. Gaszner, M. Sasvari-Szekely, A. Guttman and H. Kalasz

Affiliation:

Keywords: doamine d4 recetor, genotyping, noninvasive dna samling, buccal swab

Abstract: A noninvasive DNA sampling method has been implemented collecting buccalmucosa cells by cotton wool swabs. An amount of 0.2-2 μg DNA per patient wasobtained after the phenol-extraction procedure and 0.2-2 ng DNA template wassufficient for PCR amplification of the polymorphic 48 basepair repeat region of dopaminereceptor D4 (DRD4) gene. PCR products were visualized during microfabricated electrophoretic separationby laser-induced fluorescent detection and automatic data registration. Initial data of genotyping drugdependentsubjects shows a relatively high ratio of heterozygotes, possessing either longer or shorter variantsbeside the common 4-repeat DRD4 allele.

Cite this article as:

Boor K., Ronai Z., Nemoda Z., Gaszner P., Sasvari-Szekely M., Guttman A. and Kalasz H., Noninvasive Genotyping of Dopamine Receptor D4 (DRD4) UsingNanograms of DNA From Substance-Dependent Patients, Current Medicinal Chemistry 2002; 9 (8) . https://dx.doi.org/10.2174/0929867024606821