Abstract
Whereas the majority of microarray applications still deal with expression analysis for gathering information about levels of gene products at certain cell states, other approaches simply ask the question whether particular genes, which are usually indicative for particular microorganisms and pathogens, are present in a sample or not. Investigations that are more detailed try to evaluate the presence of particular subtypes of a given pathogen. The combination of microarray technology and virus diagnostics promises to generate an ideal platform for fast, sensitive, specific, and parallelized virus diagnostics. Performing virus diagnostics on microarrays, however, requires other basic techniques to be optimized. This is necessary in order to obtain unambiguous and reproducible results, which are compatible with the needs for clinical routine. Parameters that have to be considered include supports, coupling chemistry, chemical oligonucleotide synthesis, signal enhancement strategies, and optimal coordination of PCR reactions, hybridizations, and signal detection, as well as interpretation strategies. Finally, considerations should be given to economic aspects, one chipone patient strategies and low integrated arrays as a custom-tailored way to fast and accurate diagnostic tools.
Keywords: virus diagnostics, dna microarrays, on-chip synthesis, chemical oligonucleotide synthesis, dna immobilization chemistry, dendrimers, probe specificity, fluorescence detection
Current Pharmaceutical Biotechnology
Title: Virus Diagnostics on Microarrays
Volume: 4 Issue: 6
Author(s): Hans-Martin Striebel, Eckhard Birch-Hirschfeld, Renate Egerer and Zeno Foldes-Papp
Affiliation:
Keywords: virus diagnostics, dna microarrays, on-chip synthesis, chemical oligonucleotide synthesis, dna immobilization chemistry, dendrimers, probe specificity, fluorescence detection
Abstract: Whereas the majority of microarray applications still deal with expression analysis for gathering information about levels of gene products at certain cell states, other approaches simply ask the question whether particular genes, which are usually indicative for particular microorganisms and pathogens, are present in a sample or not. Investigations that are more detailed try to evaluate the presence of particular subtypes of a given pathogen. The combination of microarray technology and virus diagnostics promises to generate an ideal platform for fast, sensitive, specific, and parallelized virus diagnostics. Performing virus diagnostics on microarrays, however, requires other basic techniques to be optimized. This is necessary in order to obtain unambiguous and reproducible results, which are compatible with the needs for clinical routine. Parameters that have to be considered include supports, coupling chemistry, chemical oligonucleotide synthesis, signal enhancement strategies, and optimal coordination of PCR reactions, hybridizations, and signal detection, as well as interpretation strategies. Finally, considerations should be given to economic aspects, one chipone patient strategies and low integrated arrays as a custom-tailored way to fast and accurate diagnostic tools.
Export Options
About this article
Cite this article as:
Striebel Hans-Martin, Birch-Hirschfeld Eckhard, Egerer Renate and Foldes-Papp Zeno, Virus Diagnostics on Microarrays, Current Pharmaceutical Biotechnology 2003; 4 (6) . https://dx.doi.org/10.2174/1389201033377274
DOI https://dx.doi.org/10.2174/1389201033377274 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
Call for Papers in Thematic Issues
Artificial Intelligence in Bioinformatics
Bioinformatics is an interdisciplinary field that analyzes and explores biological data. This field combines biology and information system. Artificial Intelligence (AI) has attracted great attention as it tries to replicate human intelligence. It has become common technology for analyzing and solving complex data and problems and encompasses sub-fields of machine ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
The Changing Face of Cytokines in the Brain: Perspectives From EAE
Current Pharmaceutical Design Dementia Etiologies and Remedies in Traditional Persian Medicine; A Review of Medicinal Plants and Phytochemistry
Current Drug Metabolism Regulation of Cytokine Production by γδ T Cells
Current Medicinal Chemistry - Anti-Inflammatory & Anti-Allergy Agents The Immunoproteasome: An Emerging Therapeutic Target
Current Topics in Medicinal Chemistry Virus-Associated Vasculitides: An Update
Current Immunology Reviews (Discontinued) Models in Research of Pharmacoresistant Epilepsy: Present and Future in Development of Antiepileptic Drugs
Current Medicinal Chemistry Alcohol Abuse and HIV Infection: Role of DRD2
Current HIV Research The Genetics of Scrapie in Sheep and Goats
Current Molecular Medicine Place of Nanofiltration for Assuring Viral Safety of Biologicals
Current Nanoscience The Role of Venous Abnormalities in Neurological Disease
Reviews on Recent Clinical Trials Multiple Sclerosis – Established and Novel Therapeutic Approaches
Central Nervous System Agents in Medicinal Chemistry The Causes and Consequences of Childhood Encephalitis in Asia
Infectious Disorders - Drug Targets Combinatorial Approaches to Iminosugars as Glycosidase and Glycosyltransferase Inhibitors
Combinatorial Chemistry & High Throughput Screening Human Parvovirus B19: An Infectious Agent with the Potential to Induce and Trigger Rheumatic Disease
Current Rheumatology Reviews The Herpesvirus Encoded dUTPase as a Potential Chemotherapeutic Target
Current Protein & Peptide Science Postoperative Care of the Transplanted Patient
Current Cardiology Reviews COVID-19 Invades Several Important Organs other than the Lungs: Organs Crosstalk
Coronaviruses The Safety of the Temozolomide in Patients with Malignant Glioma
Current Drug Safety Mouse Models of Autoimmune Uveitis
Current Pharmaceutical Design Recent Achievements in Understanding Immune Recovery of Children Treated with HAART
Current Pediatric Reviews