Determination of the Antiretroviral Drug Nevirapine in Diluted Alkaline Electrolyte by Adsorptive Stripping Voltammetry at the Mercury Film Electrode

Arnaldo      Aguiar Castro; Ricardo      Queiroz Aucelio; Nicolas      Adrian Rey; Eliane      Monsores Miguel; Percio      Augusto Mardini Farias

Abstract

This paper describes a stripping method for the determination of nevirapine at the submicromolar concentration levels The method is based on controlled adsorptive accumulation of nevirapine at thin-film mercury electrode, followed by a linear cyclic scan voltammetry measurement of the surface species. Optimal experimental conditions include a 2.0 × 10-3 mol L-1 NaOH solution (supporting electrolyte), an accumulation potential of -0.20 V, and a scan rate of 100 mV s-1. The response of nevirapine is linear over the concentration range 0.01 – 0.14 ppm. For an accumulation time of 6 minutes, the detection limit was found to be 0.87 ppb (3.0 × 10- 9 mol L-1). More convenient methods to measure the nevirapine in presence of the efavirenz, acyclovir, didanosine, indinavir, nelfinavir, saquinavir, lamivudine, zidovudine and metals ions were also investigated. The utility of this method is demonstrated by the presence of nevirapine together with ATP or DNA.

Keywords: Nevirapine determination, metals ions, efavirenz, acyclovir, didanosine, indinavir, nelfinavir, saquinavir, lamivudine, zidovudine, ATP, DNA, thin-film mercury electrode, linear cyclic scan stripping voltammetry, NNRTI, Houston Ametek-DMP-40 series digital plotter, Milli-Q, negative-going potential scan, voltammogram, Co-nevirapine complex, HIV

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Combinatorial Chemistry & High Throughput Screening

Title: Determination of the Antiretroviral Drug Nevirapine in Diluted Alkaline Electrolyte by Adsorptive Stripping Voltammetry at the Mercury Film Electrode

Volume: 14 Issue: 1

Author(s): Arnaldo Aguiar Castro, Ricardo Queiroz Aucelio, Nicolas Adrian Rey, Eliane Monsores Miguel and Percio Augusto Mardini Farias

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Abstract: This paper describes a stripping method for the determination of nevirapine at the submicromolar concentration levels The method is based on controlled adsorptive accumulation of nevirapine at thin-film mercury electrode, followed by a linear cyclic scan voltammetry measurement of the surface species. Optimal experimental conditions include a 2.0 × 10-3 mol L-1 NaOH solution (supporting electrolyte), an accumulation potential of -0.20 V, and a scan rate of 100 mV s-1. The response of nevirapine is linear over the concentration range 0.01 – 0.14 ppm. For an accumulation time of 6 minutes, the detection limit was found to be 0.87 ppb (3.0 × 10- 9 mol L-1). More convenient methods to measure the nevirapine in presence of the efavirenz, acyclovir, didanosine, indinavir, nelfinavir, saquinavir, lamivudine, zidovudine and metals ions were also investigated. The utility of this method is demonstrated by the presence of nevirapine together with ATP or DNA.

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Aguiar Castro Arnaldo, Queiroz Aucelio Ricardo, Adrian Rey Nicolas, Monsores Miguel Eliane and Augusto Mardini Farias Percio, Determination of the Antiretroviral Drug Nevirapine in Diluted Alkaline Electrolyte by Adsorptive Stripping Voltammetry at the Mercury Film Electrode, Combinatorial Chemistry & High Throughput Screening 2011; 14 (1) . https://dx.doi.org/10.2174/1386207311107010022

DOI https://dx.doi.org/10.2174/1386207311107010022	Print ISSN 1386-2073
Publisher Name Bentham Science Publisher	Online ISSN 1875-5402

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