Abstract
For system-level understanding of various cellular events, it is vital to identify all molecules participating in each process and understand their interactions in a quantitative manner with spatiotemporal resolution. The advent of recent proteomics approaches has enabled large scale analyses of the quantities and interactions of proteins, the most important biomolecules, in the budding yeast Saccharomyces cerevisiae. In particular, differential protein expression analysis can be achieved by mass spectrometry with stable isotope labeling either in vitro or in vivo to quantify relative difference in protein abundance. Furthermore, researchers are further developing these techniques for absolute quantification of proteins as well as their modifications and interactions, aiming to grasp more precise pictures of the underlying molecular mechanisms for the system-level understanding. Here we review recent advance of quantitative proteomic approaches, focusing on those using mass spectrometry.
Keywords: quantitative proteomics, mass spectrometry, stable isotope, absolute quantification
Current Genomics
Title: Mass Spectrometry-Based Proteomics for Quantitative Description of Cellular Events
Volume: 5 Issue: 8
Author(s): Keiji Kito and Takashi Ito
Affiliation:
Keywords: quantitative proteomics, mass spectrometry, stable isotope, absolute quantification
Abstract: For system-level understanding of various cellular events, it is vital to identify all molecules participating in each process and understand their interactions in a quantitative manner with spatiotemporal resolution. The advent of recent proteomics approaches has enabled large scale analyses of the quantities and interactions of proteins, the most important biomolecules, in the budding yeast Saccharomyces cerevisiae. In particular, differential protein expression analysis can be achieved by mass spectrometry with stable isotope labeling either in vitro or in vivo to quantify relative difference in protein abundance. Furthermore, researchers are further developing these techniques for absolute quantification of proteins as well as their modifications and interactions, aiming to grasp more precise pictures of the underlying molecular mechanisms for the system-level understanding. Here we review recent advance of quantitative proteomic approaches, focusing on those using mass spectrometry.
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Cite this article as:
Kito Keiji and Ito Takashi, Mass Spectrometry-Based Proteomics for Quantitative Description of Cellular Events, Current Genomics 2004; 5 (8) . https://dx.doi.org/10.2174/1389202043348616
DOI https://dx.doi.org/10.2174/1389202043348616 |
Print ISSN 1389-2029 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5488 |
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