Fluorescent Chiral Derivatization Reagents Possessing Benzofurazan Structure for the Resolution of Optical Isomers in HPLC: The Synthesis, Characteristics and Application

Title: Fluorescent Chiral Derivatization Reagents Possessing Benzofurazan Structure for the Resolution of Optical Isomers in HPLC: The Synthesis, Characteristics and Application

Volume: 1 Issue: 1

Author(s): Toshimasa Toyo'oka

Affiliation:

Keywords: benzofurazan fluorophore, chiral resolution, biological important molecule, diastereomer formation, hplc analysis

Abstract: Indirect resolution of chiral molecules, based upon pre-column derivatization and diastereomer formation using benzofurazan bearing chiral labeling reagents, by high-performance liquid chromatography are described in this minireview. The synthesis, characteristics and application of the fluorescent chiral derivatization reagents for various functional groups, i.e. amine (NBD-PyNCS, DBD-PyNCS, DBD-β-Pro, DBD-hydroxyproline), carboxyl (NBD-APy, DBD-APy, ABD-APy), carbonyl (NBD-ProCZ, DBD-ProCZ), hydroxyl (NBD-Pro-COCl, DBD-Pro-COCl) and thiol, etc., are including in the text.

Cite this article as:

Toyo'oka Toshimasa, Fluorescent Chiral Derivatization Reagents Possessing Benzofurazan Structure for the Resolution of Optical Isomers in HPLC: The Synthesis, Characteristics and Application, Current Pharmaceutical Analysis 2005; 1 (1) . https://dx.doi.org/10.2174/1573412052953364