Abstract
Apoptosis, induced in tumors by anticancer agents, is characterized by caspase activation, impaired cellular respiration and decreased cellular ATP. Respiration in Jurkat and HL-60 cells treated with doxorubicin, dactinomycin or platinum drugs is measured using a Pd(II) phosphor that monitors [O2] in cell suspensions as a function of time. Cellular ATP is determined using the luciferin-luciferase bioluminescence system. Intracellular caspase activation is measured by allowing caspases to cleave Ac-DEVD-AFC to the fluorescent AFC, which is detected on HPLC. Comparing the ways in which respiration, ATP level, and caspase activity vary with time points up differences between the mechanisms of actions of doxorubicin, dactinomycin and the platinum compounds. These methods accurately determine the sensitivity of tumors to anticancer drugs.
Keywords: Apoptosis, cellular ATP, 7-amino-4-trifluoromethyl coumarin, pan-caspase inhibitor, doxorubicin
Current Drug Therapy
Title: Study on Caspase-Induced Mitochondrial Dysfunction by Anticancer Drugs
Volume: 2 Issue: 3
Author(s): Zhimin Tao, Matthew P. Morrow, Harvey S. Penefsky, Jerry Goodisman and Abdul-Kader Souid
Affiliation:
Keywords: Apoptosis, cellular ATP, 7-amino-4-trifluoromethyl coumarin, pan-caspase inhibitor, doxorubicin
Abstract: Apoptosis, induced in tumors by anticancer agents, is characterized by caspase activation, impaired cellular respiration and decreased cellular ATP. Respiration in Jurkat and HL-60 cells treated with doxorubicin, dactinomycin or platinum drugs is measured using a Pd(II) phosphor that monitors [O2] in cell suspensions as a function of time. Cellular ATP is determined using the luciferin-luciferase bioluminescence system. Intracellular caspase activation is measured by allowing caspases to cleave Ac-DEVD-AFC to the fluorescent AFC, which is detected on HPLC. Comparing the ways in which respiration, ATP level, and caspase activity vary with time points up differences between the mechanisms of actions of doxorubicin, dactinomycin and the platinum compounds. These methods accurately determine the sensitivity of tumors to anticancer drugs.
Export Options
About this article
Cite this article as:
Tao Zhimin, Morrow P. Matthew, Penefsky S. Harvey, Goodisman Jerry and Souid Abdul-Kader, Study on Caspase-Induced Mitochondrial Dysfunction by Anticancer Drugs, Current Drug Therapy 2007; 2 (3) . https://dx.doi.org/10.2174/157488507781695621
DOI https://dx.doi.org/10.2174/157488507781695621 |
Print ISSN 1574-8855 |
Publisher Name Bentham Science Publisher |
Online ISSN 2212-3903 |
Call for Papers in Thematic Issues
Novel Therapeutic Approaches and Biomarkers for Chronic Kidney Disease (CKD)
The thematic issue on "Novel Therapeutic Approaches and Biomarkers for CKD" aims to provide a comprehensive exploration of cutting-edge strategies for the management of chronic kidney disease (CKD). This issue will delve into emerging therapeutic targets, focusing on critical aspects such as podocyte injury, endothelial dysfunction, and tubulointerstitial fibrosis, which ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
The Emerging Pharmacology of TRPM8 Channels: Hidden Therapeutic Potential Underneath a Cold Surface
Current Pharmaceutical Biotechnology Chronopharmaceutics: A Promising Drug Delivery Finding of the Last Two Decades
Recent Patents on Drug Delivery & Formulation Heat Shock Proteins (HSPs): A Novel Target for Cancer Metastasis Prevention
Current Drug Targets Cyclodextrins and their Derivatives as Carrier Molecules in Drug and Gene Delivery Systems
Current Organic Chemistry Development of Environment-Friendly Insecticides Based on Enantioselectivity: Bifenthrin as a Case
Current Protein & Peptide Science Small-molecule Antiviral Agents in Ongoing Clinical Trials for COVID-19
Current Drug Targets Optimization and Characterization of Biogenic Silver Nanoparticles Synthesized by Leaves Extract of <i>Alphonsea madraspatana</i>
Current Bioactive Compounds MicroRNAs and Peroxisome Proliferator-Activated Receptors Governing the Differentiation of Mesenchymal Stem Cells
Current Stem Cell Research & Therapy Pavlovian Sign-Tracking Model of Alcohol Abuse
Current Drug Abuse Reviews Fragment-Based Drug Discovery and Molecular Docking in Drug Design
Current Pharmaceutical Biotechnology Covid-19 Susceptibility and Severity Might be Modified by Vitamin D Status: Theoretical and Practical Considerations
Current Respiratory Medicine Reviews Silybin and Silymarin - New and Emerging Applications in Medicine
Current Medicinal Chemistry The Role of Hyphenated Chromatography-Mass Spectrometry Techniques in Exploratory Drug Metabolism and Pharmacokinetics
Current Pharmaceutical Design Modulation of pRb/E2F Functions in the Regulation of Cell Cycle and in Cancer
Current Cancer Drug Targets Mass Spectrometry Imaging: Applications in Drug Distribution Studies
Current Drug Metabolism The Potential of Secondary Metabolites from Plants as Drugs or Leads Against Protozoan Neglected Diseases – Part I
Current Medicinal Chemistry Metabotyping of a Protected Non-Model Organism, Green Sea Turtle (Chelonia mydas), using <sup>1</sup>H NMR Spectroscopy and Optimized Plasma Methods for Metabolomics
Current Metabolomics Plant Coumestans: Recent Advances and Future Perspectives in Cancer Therapy
Anti-Cancer Agents in Medicinal Chemistry Effect of Recombinant Human Thymosin-α1, an Immuno-Modulating Peptide with 28 Amino Acids, on the Activity of Cytochrome P450s
Drug Metabolism Letters Induction of Cytochrome P450 3A4 and P-Glycoprotein by the Isoxazolyl- Penicillin Antibiotic Flucloxacillin
Current Drug Metabolism