Abstract
Protein phosphorylation is one of the most important post-translational events in cell regulation and signal transduction. Since the isolation of phosphorylated peptides from biological sources is usually not feasible, there is a need for efficient chemical phosphorylation methods to allow the study of the role of phosphorylation/dephosphorylation in biological processes. The recent developments in phosphopeptide chemistry (special protecting groups for the phosphate moiety and new amidite reagents) have provided peptide chemists with a wide variety of different strategies applicable for work with the sensitive phosphoserine, phosphothreonine and phosphotyrosine-containing peptides. This has made possible the efficient chemical preparation of longer, multiply phosphorylated, possibly cell-permeable or fluorescent residue-bearing peptides.
Keywords: Phosphorylation, tert-butyldimethylsilyl, Phosphate Protecting Groups, PSD/MALDI, collision-induced dissociation(CID)
Current Organic Chemistry
Title: Phosphopeptides - Chemical Synthesis, Analysis, Outlook and Limitations.
Volume: 11 Issue: 5
Author(s): G. K. Toth, Z. Kele and G. Varadi
Affiliation:
Keywords: Phosphorylation, tert-butyldimethylsilyl, Phosphate Protecting Groups, PSD/MALDI, collision-induced dissociation(CID)
Abstract: Protein phosphorylation is one of the most important post-translational events in cell regulation and signal transduction. Since the isolation of phosphorylated peptides from biological sources is usually not feasible, there is a need for efficient chemical phosphorylation methods to allow the study of the role of phosphorylation/dephosphorylation in biological processes. The recent developments in phosphopeptide chemistry (special protecting groups for the phosphate moiety and new amidite reagents) have provided peptide chemists with a wide variety of different strategies applicable for work with the sensitive phosphoserine, phosphothreonine and phosphotyrosine-containing peptides. This has made possible the efficient chemical preparation of longer, multiply phosphorylated, possibly cell-permeable or fluorescent residue-bearing peptides.
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Cite this article as:
Toth K. G., Kele Z. and Varadi G., Phosphopeptides - Chemical Synthesis, Analysis, Outlook and Limitations., Current Organic Chemistry 2007; 11 (5) . https://dx.doi.org/10.2174/138527207780059295
DOI https://dx.doi.org/10.2174/138527207780059295 |
Print ISSN 1385-2728 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5348 |
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