Abstract
Bovine pancreatic deoxyribonuclease I (bpDNase), the first DNase discovered, is the best characterized among various types of DNase. A catalytic mechanism has been suggested based on the X-ray structure of the bpDNase-octamer complex. In this review, we will focus on three aspects: 1) the distinctive functions of the two structural calcium atoms; 2) the biological functions of the two disulfides; and 3) the involvement of the N- and C-terminal fragments in the enzyme folding for activity.
Keywords: Deoxyribonuclease, calcium, disulfide, protein folding, chemical modification, site-directed mutagenesis, enzyme mechanism
Protein & Peptide Letters
Title: Structure and Function of Bovine Pancreatic Deoxyribonuclease I
Volume: 13 Issue: 5
Author(s): Wei-Jung Chen and Ta-Hsiu Liao
Affiliation:
Keywords: Deoxyribonuclease, calcium, disulfide, protein folding, chemical modification, site-directed mutagenesis, enzyme mechanism
Abstract: Bovine pancreatic deoxyribonuclease I (bpDNase), the first DNase discovered, is the best characterized among various types of DNase. A catalytic mechanism has been suggested based on the X-ray structure of the bpDNase-octamer complex. In this review, we will focus on three aspects: 1) the distinctive functions of the two structural calcium atoms; 2) the biological functions of the two disulfides; and 3) the involvement of the N- and C-terminal fragments in the enzyme folding for activity.
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Cite this article as:
Chen Wei-Jung and Liao Ta-Hsiu, Structure and Function of Bovine Pancreatic Deoxyribonuclease I, Protein & Peptide Letters 2006; 13 (5) . https://dx.doi.org/10.2174/092986606776819475
DOI https://dx.doi.org/10.2174/092986606776819475 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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