Abstract
Assembly of α-synuclein (α-Syn) into neurotoxic oligomers and fibrils is an important pathogenic feature of Parkinsons disease. Studying the early events of α-Syn aggregation, such as oligomerization and nucleation, is indispensable to understanding the complicated process. Here, photo-induced cross-linking of unmodified proteins (PICUP) technique is applied to elucidate the early-stage oligomerization of α-Syn. Results show that α-Syn in solution exhibits a mixture of various species, including at least monomers, dimers and trimers. Aggregation of α-Syn probably originates from the dimeric and trimeric seeds. Furthermore, the N-terminal amphipathic region is proposed to be required for the oligomerization (dimerization and trimerization) process. This observation may extend our knowledge on the early events of α-Syn aggregation and the neurotoxic aggregation species.
Keywords: α-synuclein, oligomerization, PICUP, dimmer, trimer
Protein & Peptide Letters
Title: The Early Events of α-Synuclein Oligomerization Revealed by Photo- Induced Cross-Linking
Volume: 13 Issue: 4
Author(s): Hong-Tao Li, Xiao-Jing Lin, Yuan-Yuan Xie and Hong-Yu Hu
Affiliation:
Keywords: α-synuclein, oligomerization, PICUP, dimmer, trimer
Abstract: Assembly of α-synuclein (α-Syn) into neurotoxic oligomers and fibrils is an important pathogenic feature of Parkinsons disease. Studying the early events of α-Syn aggregation, such as oligomerization and nucleation, is indispensable to understanding the complicated process. Here, photo-induced cross-linking of unmodified proteins (PICUP) technique is applied to elucidate the early-stage oligomerization of α-Syn. Results show that α-Syn in solution exhibits a mixture of various species, including at least monomers, dimers and trimers. Aggregation of α-Syn probably originates from the dimeric and trimeric seeds. Furthermore, the N-terminal amphipathic region is proposed to be required for the oligomerization (dimerization and trimerization) process. This observation may extend our knowledge on the early events of α-Syn aggregation and the neurotoxic aggregation species.
Export Options
About this article
Cite this article as:
Li Hong-Tao, Lin Xiao-Jing, Xie Yuan-Yuan and Hu Hong-Yu, The Early Events of α-Synuclein Oligomerization Revealed by Photo- Induced Cross-Linking, Protein & Peptide Letters 2006; 13 (4) . https://dx.doi.org/10.2174/092986606775974384
DOI https://dx.doi.org/10.2174/092986606775974384 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Plasma Clusterin as a Potential Biomarker for Alzheimer’s Disease-A Systematic Review and Meta-analysis
Current Alzheimer Research The Influence of Metabolism on the MAO-B Inhibitory Potency of Selegiline
Current Medicinal Chemistry Editorial (Thematic Issue: Current Update on Association Between Alzheimer’s Disease and Type 2 Diabetes: Volume I)
CNS & Neurological Disorders - Drug Targets Editorial: Generation Rx: Adolescent Prescription Drug Abuse
Current Drug Abuse Reviews Cytotoxic and Radio-sensitizing Effects of Polyphenolic Acetates in a Human Glioma Cell Line (BMG-1)
Current Pharmaceutical Design Mediterranean Diet and Dementia of the Alzheimer Type
Current Aging Science Increased Expression of the Remodeling- and Tumorigenic-Associated Factor Osteopontin in Pyramidal Neurons of the Alzheimers Disease Brain
Current Alzheimer Research Importance of Influx and Efflux Systems and Xenobiotic Metabolizing Enzymes in Intratumoral Disposition of Anticancer Agents
Current Cancer Drug Targets Gene-Environment Interactions Underlying the Effect of Cannabis in First Episode Psychosis
Current Pharmaceutical Design Benzofuran: A Key Heterocycle - Ring Closure And Beyond
Mini-Reviews in Organic Chemistry Further Evaluation of Mechanisms Associated with the Antidepressantlike Signature of Scopolamine in Mice
CNS & Neurological Disorders - Drug Targets An update on Anti-inflammatory Compounds: A Review
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Impact of Diabetes in Blood-Testis and Blood-Brain Barriers: Resemblances and Differences
Current Diabetes Reviews Prognostic Value of Cystatin C in Acute Ischemic Stroke Patients with Intravenous Thrombolysis
Current Neurovascular Research Pharmacological Treatment of Obesity: Current Standards and Future Perspectives
Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry (Discontinued) “SLY AS A FOXO”: New Paths with Forkhead Signaling in the Brain
Current Neurovascular Research Synthesis and Preliminary Screening of Novel Tryptamines as 5-HT4 Receptor Ligands
Current Medicinal Chemistry Psychological Stress in Pathogenesis of Essential Hypertension
Current Hypertension Reviews Editorial (Thematic Issue:Classic and New Agents to the Treatment of Affective Disorders: Some Concerns and Perspectives
Current Psychopharmacology Inflammatory Aspects of Depression
Inflammation & Allergy - Drug Targets (Discontinued)