Abstract
PP5715 is a putative tumor suppressor gene that encodes a protein of 199 amino acids. Expression of PP5715 in E. coli was mainly as inclusion bodies. The recombinant protein was purified by a NTA-Ni2+ affinity column, refolded by dialysis, and shown to suppress growth of two human hepatocellular carcinoma cell lines using the MTT assay or the clonogenic assays. Computer analysis and fusion expression of PP5175 and GFP showed that PP5715 may be a secretory protein. It may bind to receptors on the membrane surface and thus induce regulation of cell growth. These preliminary results suggest that protein PP5715 may be a new tumor suppressor with growth inhibition effects on hepatocellular carcinoma cells.
Keywords: Tumor suppressor gene, PP5715, expression and purification, cell growth inhibition, subcellular localization
Protein & Peptide Letters
Title: Expression and Purification of a Putative Tumor Suppressor Gene PP5715 in E. coli with Growth Inhibition of Hepatocellular Carcinoma Cells
Volume: 13 Issue: 1
Author(s): M. Wen-Zhe, H. Wei, Q. Wen-Xin, W. Da-Fang, G. Jian-Ren, Y. Sheng-Li and G. Yi
Affiliation:
Keywords: Tumor suppressor gene, PP5715, expression and purification, cell growth inhibition, subcellular localization
Abstract: PP5715 is a putative tumor suppressor gene that encodes a protein of 199 amino acids. Expression of PP5715 in E. coli was mainly as inclusion bodies. The recombinant protein was purified by a NTA-Ni2+ affinity column, refolded by dialysis, and shown to suppress growth of two human hepatocellular carcinoma cell lines using the MTT assay or the clonogenic assays. Computer analysis and fusion expression of PP5175 and GFP showed that PP5715 may be a secretory protein. It may bind to receptors on the membrane surface and thus induce regulation of cell growth. These preliminary results suggest that protein PP5715 may be a new tumor suppressor with growth inhibition effects on hepatocellular carcinoma cells.
Export Options
About this article
Cite this article as:
Wen-Zhe M., Wei H., Wen-Xin Q., Da-Fang W., Jian-Ren G., Sheng-Li Y. and Yi G., Expression and Purification of a Putative Tumor Suppressor Gene PP5715 in E. coli with Growth Inhibition of Hepatocellular Carcinoma Cells, Protein & Peptide Letters 2006; 13 (1) . https://dx.doi.org/10.2174/092986606774502171
DOI https://dx.doi.org/10.2174/092986606774502171 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Current Methods and Research Progress in Nanomaterials Risk Assessment
Current Drug Metabolism Mechanisms of Tubulin Binding Ligands to Target Cancer Cells: Updates on their Therapeutic Potential and Clinical Trials
Current Cancer Drug Targets Applications of High Content Screening in Life Science Research
Combinatorial Chemistry & High Throughput Screening Cause and Consequences of Genetic and Epigenetic Alterations in Human Cancer
Current Genomics Immune Check Point Inhibitors Combination in Melanoma: Worth the Toxicity?
Reviews on Recent Clinical Trials Herbal Medicines for Diabetes Management and its Secondary Complications
Current Diabetes Reviews Main Nutritional and Environmental Risk Factors in Children with Leukemia from a Public Hospital of the State of Guanajuato, Mexico
Current Cancer Therapy Reviews Advantages of Nanotechnology- Based Chinese Herb Drugs on Biological Activities
Current Drug Metabolism Shutting Down the Furnace: Preferential Killing of Cancer Cells with Mitochondrial-Targeting Molecules
Current Medicinal Chemistry Shaping the Genome with Non-Coding RNAs
Current Genomics The Antidepressant-like Effects of Estrogen-mediated Ghrelin
Current Neuropharmacology IAP Proteins Antagonist: An Introduction and Chemistry of Smac Mimetics under Clinical Development
Current Medicinal Chemistry Therapeutic Gene Products Delivery by Neuron Stem Cells
Current Pharmaceutical Biotechnology Adeno-associated Viral Vectors for Correction of Inborn Errors of Metabolism: Progressing Towards Clinical Application
Current Pharmaceutical Design Gene Therapy and Targeted Toxins for Glioma
Current Gene Therapy Phospholipase D Inhibition: Beneficial and Harmful Consequences for a Double-Dealer Enzyme
Current Enzyme Inhibition The Future of Diabetes Management by Healthy Probiotic Microorganisms
Current Diabetes Reviews Analysis of Single-Cell RNA-seq Data by Clustering Approaches
Current Bioinformatics Drug Delivery Systems for the Treatment of Diabetes Mellitus: State of the Art
Current Pharmaceutical Design Phagoptosis - Cell Death By Phagocytosis - Plays Central Roles in Physiology, Host Defense and Pathology
Current Molecular Medicine