Abstract
Amyloidosis refers to a heterogeneous group of disorders characterized by the extracellular deposition of amyloid proteins in various tissues of the body. About 25 different amyloid proteins originating from presumably normal precursor proteins have been described so far. Identification of the chemical nature of amyloid is necessary in clinical practice, since both prognosis and treatment regimens are different in the various amyloidoses. This review is aimed at summarizing the new biochemical micro-techniques for precise typing of amyloid proteins in diagnostic biopsy specimens. The reported micro-techniques vary with respect to the state of the amyloid-bearing tissue, i.e., unfixed-frozen or formalin-fixed, the purification strategy (chromatographic or electrophoretic) and the analytical method used (ELISA, Western blotting, amino acid sequencing, mass spectroscopy). The utility and advantages of the described protocols are discussed with regard to tissue state, sample size, amyloid content, simplicity of procedures and possible applicability in diagnostic laboratories.
Keywords: Amyloid, Extraction, Micro-techniques, Biopsy
Current Pharmaceutical Analysis
Title: Biochemical Micro-Techniques in the Diagnosis and Classification of Amyloidosis
Volume: 2 Issue: 1
Author(s): Batia Kaplan, Brian M. Martin, Avi Livneh, Mordechai Pras and Gloria Gallo
Affiliation:
Keywords: Amyloid, Extraction, Micro-techniques, Biopsy
Abstract: Amyloidosis refers to a heterogeneous group of disorders characterized by the extracellular deposition of amyloid proteins in various tissues of the body. About 25 different amyloid proteins originating from presumably normal precursor proteins have been described so far. Identification of the chemical nature of amyloid is necessary in clinical practice, since both prognosis and treatment regimens are different in the various amyloidoses. This review is aimed at summarizing the new biochemical micro-techniques for precise typing of amyloid proteins in diagnostic biopsy specimens. The reported micro-techniques vary with respect to the state of the amyloid-bearing tissue, i.e., unfixed-frozen or formalin-fixed, the purification strategy (chromatographic or electrophoretic) and the analytical method used (ELISA, Western blotting, amino acid sequencing, mass spectroscopy). The utility and advantages of the described protocols are discussed with regard to tissue state, sample size, amyloid content, simplicity of procedures and possible applicability in diagnostic laboratories.
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Cite this article as:
Kaplan Batia, Martin M. Brian, Livneh Avi, Pras Mordechai and Gallo Gloria, Biochemical Micro-Techniques in the Diagnosis and Classification of Amyloidosis, Current Pharmaceutical Analysis 2006; 2 (1) . https://dx.doi.org/10.2174/157341206775473981
DOI https://dx.doi.org/10.2174/157341206775473981 |
Print ISSN 1573-4129 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-676X |
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