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Current Drug Discovery Technologies

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ISSN (Print): 1570-1638
ISSN (Online): 1875-6220

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Research Article

Effects of Combined Garcinia kola and Kigelia africana on Insulin and Paraoxonase 1 (PON1) Levels in Type 2 Diabetic Rats

Author(s): Adams Omoaghe*, Olusoji Oyesola, Tony Ezike, Blessing Omizu and Kukoyi Boone

Volume 19, Issue 5, 2022

Published on: 27 July, 2022

Article ID: e080422203277 Pages: 9

DOI: 10.2174/1570163819666220408100011

Price: $65

Abstract

Background: Individual extracts of Garcinia kola and Kigelia africana have been shown to have therapeutic effects against a variety of variables linked to the development of diabetes mellitus. However, there is still a lack of information about the combined effects of these extracts on Insulin and Paraoxonase 1 (PON-1) in Streptozotocin-Nicotinamide-induced type-2 diabetic Wistar rats.

Methods: Forty-two young male rats (180-200g) were randomly divided into six groups (n = 7/group). Diabetes was intraperitoneally induced with 110 mg/kg of nicotinamide constituted in distilled water and fifteen minutes later with 65 mg/kg of streptozocin freshly prepared in 0.1M citrate buffer (pH of 4.5) and treated for six weeks as follows: the control rats received either 0.9% normal saline (NS) or 250 mg/kg extract by gavage. The remaining animals were diabetes induced and subsequently treated with either NS, graded doses of the extract (250 mg/kg and 500 mg/kg), or 5 mg/kg Glibenclamide + 100mg/kg Metformin. Gas chromatography-mass spectrometry (GCMS) of the combined extracts was also analyzed to identify the bioactive compounds present in it. Insulin, PON-1 levels, lipid profiles, and atherogenic index were assessed.

Results: Our findings show that Insulin and PON-1 levels in the plasma of diabetic rats treated with the combined extracts were significantly increased when compared to the control rats. Moreover, the GCMS of the extract shows the presence of both monounsaturated (oleic acid) and polyunsaturated (linoleic acid) fatty acids.

Conclusion: The current findings suggest that the extract may help improve glucose homeostasis and prevent atherosclerosis through the established mechanism of the identified bioactive compounds.

Keywords: Diabetes, insulin, paraoxonase 1 (PON-1), Garcinia kola, Kigelia africana, pancreatic β-cells.

Graphical Abstract

[1]
Roden M, Shulman GI. The integrative biology of type 2 diabetes. Nature 2019; 576(7785): 51-60.
[http://dx.doi.org/10.1038/s41586-019-1797-8] [PMID: 31802013]
[2]
Ojiako OA, Chikezie PC, Ogbuji AC. Blood glucose level and lipid profile of alloxan-induced hyperglycemic rats treated with single and combinatorial herbal formulations. J Tradit Complement Med 2015; 6(2): 184-92.
[http://dx.doi.org/10.1016/j.jtcme.2014.12.005] [PMID: 27114943]
[3]
Jung UJ, Park YB, Kim SR, Choi M-S. Supplementation of persimmon leaf ameliorates hyperglycemia, dyslipidemia and hepatic fat accumulation in type 2 diabetic mice. PLoS One 2012; 7(11): e49030.
[http://dx.doi.org/10.1371/journal.pone.0049030] [PMID: 23145054]
[4]
Kelly CM, Smith RD, Williams CM. Dietary monounsaturated fatty acids and haemostasis. Proc Nutr Soc 2001; 60(2): 161-70.
[http://dx.doi.org/10.1079/PNS200087] [PMID: 11681631]
[5]
Monnier L, Lapinski H, Colette C. Contributions of fasting and postprandial plasma glucose increments to the overall diurnal hyperglycemia of type 2 diabetic patients: Variations with increasing levels of HbA(1c). Diabetes Care 2003; 26(3): 881-5.
[http://dx.doi.org/10.2337/diacare.26.3.881] [PMID: 12610053]
[6]
Soriguer F, Esteva I, Rojo-Martinez G, et al. Oleic acid from cooking oils is associated with lower insulin resistance in the general population (Pizarra study). Eur J Endocrinol 2004; 150(1): 33-9.
[http://dx.doi.org/10.1530/eje.0.1500033] [PMID: 14713277]
[7]
Mesa García MD, Aguilera García CM, Gil Hernández A. Importance of lipids in the nutritional treatment of inflammatory diseases. Nutr Hosp 2006; 21(Suppl. 2): 28-41, 30-43.
[PMID: 16771071]
[8]
DiNicolantonio JJ, O’Keefe JH. Effects of dietary fats on blood lipids: A review of direct comparison trials. Open Heart 2018; 5(2): e000871.
[http://dx.doi.org/10.1136/openhrt-2018-000871] [PMID: 30094038]
[9]
Vessby B, Aro A, Skarfors E, Berglund L, Salminen I, Lithell H. The risk to develop NIDDM is related to the fatty acid composition of the serum cholesterol esters. Diabetes 1994; 43(11): 1353-7.
[http://dx.doi.org/10.2337/diab.43.11.1353] [PMID: 7926311]
[10]
Guadarrama-López AL, Valdés-Ramos R, Martínez-Carrillo BE. Type 2 diabetes, PUFAs, and vitamin D: Their relation to inflammation. J Immunol Res 2014; 2014: 860703.
[http://dx.doi.org/10.1155/2014/860703] [PMID: 24741627]
[11]
Suresh Y, Das UN. Long-chain polyunsaturated fatty acids and chemically induced diabetes mellitus. Effect of ω-3 fatty acids. Nutrition 2003; 19(3): 213-28.
[http://dx.doi.org/10.1016/S0899-9007(02)00855-9] [PMID: 12620523]
[12]
Wu JHY, Marklund M, Imamura F, et al. Cohorts for Heart and Aging Research in Genomic Epidemiology (CHARGE) Fatty Acids and Outcomes Research Consortium (FORCE). Omega-6 fatty acid biomarkers and incident type 2 diabetes: Pooled analysis of individual-level data for 39 740 adults from 20 prospective cohort studies. Lancet Diabetes Endocrinol 2017; 5(12): 965-74.
[http://dx.doi.org/10.1016/S2213-8587(17)30307-8] [PMID: 29032079]
[13]
Johnson GH, Fritsche K. Effect of dietary linoleic acid on markers of inflammation in healthy persons: A systematic review of randomized controlled trials. J Acad Nutr Diet 2012; 112(7): 1029- 1041, 1041.e1-1041.e15.
[http://dx.doi.org/10.1016/j.jand.2012.03.029] [PMID: 22889633]
[14]
Serhan CN, Chiang N, Dalli J, Levy BD. Lipid mediators in the resolution of inflammation. Cold Spring Harb Perspect Biol 2014; 7(2): a016311.
[http://dx.doi.org/10.1101/cshperspect.a016311] [PMID: 25359497]
[15]
Iwu MM, Igboko OA, Okunji CO, Tempesta MS. Antidiabetic and aldose reductase activities of biflavanones of Garcinia kola. J Pharm Pharmacol 1990; 42(4): 290-2.
[http://dx.doi.org/10.1111/j.2042-7158.1990.tb05412.x] [PMID: 1974302]
[16]
Atawodi SEO, Olowoniyi OD. Pharmacological and therapeutic activities of Kigelia africana (Lam.) Benth. Annu Res Rev Biol 2015; 5(1): 1-17.
[http://dx.doi.org/10.9734/ARRB/2015/8632]
[17]
Masiello P, Broca C, Gross R, et al. Experimental NIDDM: Development of a new model in adult rats administered streptozotocin and nicotinamide. Diabetes 1998; 47(2): 224-9.
[http://dx.doi.org/10.2337/diab.47.2.224] [PMID: 9519717]
[18]
Yeo J, Kang YM, Cho SI, Jung MH. Effects of a multi-herbal extract on type 2 diabetes. Chin Med 2011; 6(1): 10.
[http://dx.doi.org/10.1186/1749-8546-6-10] [PMID: 21375727]
[19]
Salahdeen HM, Omoaghe AO, Isehunwa GO, Murtala BA, Alada ARA. Gas chromatography mass spectrometry (GC-MS) analysis of ethanolic extracts of kolanut ( Cola nitida )(vent) and its toxicity studies in rats. J Med Plants Res 2015; 9(3): 56-70.
[http://dx.doi.org/10.5897/JMPR2014.5711]
[20]
Marks V, Dawson A. Rapid stick method for determining blood-glucose concentration. BMJ 1965; 1(5430): 293-4.
[http://dx.doi.org/10.1136/bmj.1.5430.293] [PMID: 14229711]
[21]
Olatunji LA, Michael OS, Adewumi FO, Aiyegboyin IJ, Olatunji VA. Combined estrogen-progestogen but not progestogen-only oral contraceptive alters glucose tolerance and plasma lipid profile in female rats. Pathophysiology 2012; 19(1): 29-34.
[http://dx.doi.org/10.1016/j.pathophys.2011.09.001] [PMID: 21943687]
[22]
Haffner SM, Miettinen H, Stern MP. The homeostasis model in the San Antonio Heart Study. Diabetes Care 1997; 20(7): 1087-92.
[http://dx.doi.org/10.2337/diacare.20.7.1087] [PMID: 9203442]
[23]
Basila AM, Hernández JM, Alarcón ML. Diagnostic methods of insulin resistance in a pediatric population. Bol Méd Hosp Infant México 2011; 68(5): 397-404.
[24]
Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972; 18(6): 499-502.
[http://dx.doi.org/10.1093/clinchem/18.6.499] [PMID: 4337382]
[25]
Shaker E, Mahmoud H, Mnaa S. Silymarin, the antioxidant component and Silybum marianum extracts prevent liver damage. Food Chem Toxicol 2010; 48(3): 803-6.
[http://dx.doi.org/10.1016/j.fct.2009.12.011] [PMID: 20034535]
[26]
Suanarunsawat T, Ayutthaya WD, Songsak T, Thirawarapan S, Poungshompoo S. Lipid-lowering and antioxidative activities of aqueous extracts of Ocimum sanctum L. leaves in rats fed with a high-cholesterol diet. Oxid Med Cell Longev 2011; 2011: 962025.
[http://dx.doi.org/10.1155/2011/962025] [PMID: 21949899]
[27]
López M, Varela L, Vázquez MJ, et al. Hypothalamic AMPK and fatty acid metabolism mediate thyroid regulation of energy balance. Nat Med 2010; 16(9): 1001-8.
[http://dx.doi.org/10.1038/nm.2207] [PMID: 20802499]
[28]
Berglund L, Lefevre M, Ginsberg HN, et al. DELTA Investigators. Comparison of monounsaturated fat with carbohydrates as a replacement for saturated fat in subjects with a high metabolic risk profile: Studies in the fasting and postprandial states. Am J Clin Nutr 2007; 86(6): 1611-20.
[http://dx.doi.org/10.1093/ajcn/86.5.1611] [PMID: 18065577]
[29]
Risérus U, Willett WC, Hu FB. Dietary fats and prevention of type 2 diabetes. Prog Lipid Res 2009; 48(1): 44-51.
[http://dx.doi.org/10.1016/j.plipres.2008.10.002] [PMID: 19032965]
[30]
Abdul-Ghani MA, Williams K, DeFronzo RA, Stern M. What is the best predictor of future type 2 diabetes? Diabetes Care 2007; 30(6): 1544-8.
[http://dx.doi.org/10.2337/dc06-1331] [PMID: 17384342]
[31]
DeFronzo RA. Lilly lecture 1987. The triumvirate: β-cell, muscle, liver. A collusion responsible for NIDDM. Diabetes 1988; 37(6): 667-87.
[http://dx.doi.org/10.2337/diab.37.6.667] [PMID: 3289989]
[32]
DeFronzo RA, Gunnarsson R, Björkman O, Olsson M, Wahren J. Effects of insulin on peripheral and splanchnic glucose metabolism in noninsulin-dependent (type II) diabetes mellitus. J Clin Invest 1985; 76(1): 149-55.
[http://dx.doi.org/10.1172/JCI111938] [PMID: 3894418]
[33]
Palomer X, Pizarro-Delgado J, Barroso E, Vázquez-Carrera M. Palmitic and oleic acid: The yin and yang of fatty acids in type 2 diabetes mellitus. Trends Endocrinol Metab 2018; 29(3): 178-90.
[http://dx.doi.org/10.1016/j.tem.2017.11.009] [PMID: 29290500]
[34]
Carla RV, Pernollet F. vanderWerf HayoMG. Environmental life cycle assessment of dietswithimprovedomega-3fattyacidprofiles. PLoS One 2016; 11(8): e0160397.
[35]
Kibiti CM, Afolayan AJ. Herbal therapy: A review of emerging pharmacological tools in the management of diabetes mellitus in Africa. Pharmacogn Mag 2015; 11(44)(Suppl. 2): S258-74.
[http://dx.doi.org/10.4103/0973-1296.166046] [PMID: 26664014]
[36]
Vessby B, Uusitupa M, Hermansen K, et al. KANWU Study. Substituting dietary saturated for monounsaturated fat impairs insulin sensitivity in healthy men and women: The KANWU Study. Diabetologia 2001; 44(3): 312-9.
[http://dx.doi.org/10.1007/s001250051620] [PMID: 11317662]
[37]
Wrede CE, Dickson LM, Lingohr MK, Briaud I, Rhodes CJ. Protein kinase B/Akt prevents fatty acid-induced apoptosis in pancreatic β-cells (INS-1). J Biol Chem 2002; 277(51): 49676-84.
[http://dx.doi.org/10.1074/jbc.M208756200] [PMID: 12393870]
[38]
Sarbolouki S, Javanbakht MH, Derakhshanian H, et al. Eicosapentaenoic acid improves insulin sensitivity and blood sugar in overweight type 2 diabetes mellitus patients: A double-blind randomised clinical trial. Singapore Med J 2013; 54(7): 387-90.
[http://dx.doi.org/10.11622/smedj.2013139] [PMID: 23900468]
[39]
Shirwaikar A, Rajendran K, Barik R. Effect of aqueous bark extract of Garuga pinnata Roxb. in streptozotocin-nicotinamide induced type-II diabetes mellitus. J Ethnopharmacol 2006; 107(2): 285-90.
[http://dx.doi.org/10.1016/j.jep.2006.03.012] [PMID: 16644162]
[40]
Mensink RP, Zock PL, Kester AD, Katan MB. Effects of dietary fatty acids and carbohydrates on the ratio of serum total to HDL cholesterol and on serum lipids and apolipoproteins: A meta-analysis of 60 controlled trials. Am J Clin Nutr 2003; 77(5): 1146-55.
[http://dx.doi.org/10.1093/ajcn/77.5.1146] [PMID: 12716665]
[41]
Kris-Etherton P, Eckel RH, Howard BV, St Jeor S, Bazzarre TL. Nutrition committee population science committee and clinical science Committee of the American Heart Association. Lyon diet heart study: Benefits of a Mediterranean-Style, National Cholesterol Education Program/American Heart Association Step I dietary pattern on cardiovascular disease. Circulation 2001; 103(13): 1823-5.
[http://dx.doi.org/10.1161/01.CIR.103.13.1823] [PMID: 11282918]
[42]
Gillingham LG, Gustafson JA, Han SY, Jassal DS, Jones PJ. High-oleic rapeseed (canola) and flaxseed oils modulate serum lipids and inflammatory biomarkers in hypercholesterolaemic subjects. Br J Nutr 2011; 105(3): 417-27.
[http://dx.doi.org/10.1017/S0007114510003697] [PMID: 20875216]
[43]
Damasceno NRT, Pérez-Heras A, Serra M, et al. Crossover study of diets enriched with virgin olive oil, walnuts or almonds. Effects on lipids and other cardiovascular risk markers. Nutr Metab Cardiovasc Dis 2011; 21(Suppl. 1): S14-20.
[http://dx.doi.org/10.1016/j.numecd.2010.12.006] [PMID: 21421296]
[44]
Høstmark AT, Haug A. Percentages of oleic acid and arachidonic acid are inversely related in phospholipids of human sera. Lipids Health Dis 2013; 12(1): 106.
[http://dx.doi.org/10.1186/1476-511X-12-106] [PMID: 23870138]
[45]
Hlais S, El-Bistami D, El Rahi B, Mattar MA, Obeid OA. Combined fish oil and high oleic sunflower oil supplements neutralize their individual effects on the lipid profile of healthy men. Lipids 2013; 48(9): 853-61.
[http://dx.doi.org/10.1007/s11745-013-3819-x] [PMID: 23888318]
[46]
Johnson M, Bradford C. Omega-3, omega-6 and omega-9 fatty acids: Implications for cardiovascular and other diseases. J Glycomics Lipidomics 2014; 4(123): 2153-0637.
[http://dx.doi.org/10.4172/2153-0637.1000123]
[47]
Dimitriadis E, Griffin M, Owens D, Johnson A, Collins P, Tomkin GH. Oxidation of low-density lipoprotein in NIDDM: Its relationship to fatty acid composition. Diabetologia 1995; 38(11): 1300-6.
[http://dx.doi.org/10.1007/BF00401762] [PMID: 8582539]
[48]
Xiao C, Dash S, Morgantini C, Hegele RA, Lewis GF. Pharmacological targeting of the atherogenic dyslipidemia complex: The next frontier in CVD prevention beyond lowering LDL cholesterol. Diabetes 2016; 65(7): 1767-78.
[http://dx.doi.org/10.2337/db16-0046] [PMID: 27329952]
[49]
Perdomo L, Beneit N, Otero YF, et al. Protective role of oleic acid against cardiovascular insulin resistance and in the early and late cellular atherosclerotic process. Cardiovasc Diabetol 2015; 14(1): 75.
[http://dx.doi.org/10.1186/s12933-015-0237-9] [PMID: 26055507]
[50]
Lou-Bonafonte JM, Gabás-Rivera C, Navarro MA, Osada J. PON1 and Mediterranean diet. Nutrients 2015; 7(6): 4068-92.
[http://dx.doi.org/10.3390/nu7064068] [PMID: 26024295]
[51]
Deakin S, Leviev I, Gomaraschi M, Calabresi L, Franceschini G, James RW. Enzymatically active paraoxonase-1 is located at the external membrane of producing cells and released by a high affinity, saturable, desorption mechanism. J Biol Chem 2002; 277(6): 4301-8.
[http://dx.doi.org/10.1074/jbc.M107440200] [PMID: 11726658]
[52]
Deakin SP, James RW. Genetic and environmental factors modulating serum concentrations and activities of the antioxidant enzyme paraoxonase-1. Clin Sci (Lond) 2004; 107(5): 435-47.
[http://dx.doi.org/10.1042/CS20040187] [PMID: 15265000]
[53]
Durrington PN, Mackness B, Mackness MI. The hunt for nutritional and pharmacological modulators of paraoxonase. Arterioscler Thromb Vasc Biol 2002; 22(8): 1248-50.
[http://dx.doi.org/10.1161/01.ATV.0000027414.34728.1F]
[54]
Lou-Bonafonte JM, Arnal C, Navarro MA, Osada J. Efficacy of bioactive compounds from extra virgin olive oil to modulate atherosclerosis development. Mol Nutr Food Res 2012; 56(7): 1043-57.
[http://dx.doi.org/10.1002/mnfr.201100668] [PMID: 22760979]
[55]
Tomás Barberán FA, Espín JC. Phenolic compounds and related enzymes as determinants of quality in fruits and vegetables. J Sci Food Agric 2001; 81(9): 853-76.
[http://dx.doi.org/10.1002/jsfa.885]
[56]
Loued S, Isabelle M, Berrougui H, Khalil A. The anti-inflammatory effect of paraoxonase 1 against oxidized lipids depends on its association with high density lipoproteins. Life Sci 2012; 90(1-2): 82-8.
[http://dx.doi.org/10.1016/j.lfs.2011.10.018] [PMID: 22067439]

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