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The Natural Products Journal

Editor-in-Chief

ISSN (Print): 2210-3155
ISSN (Online): 2210-3163

Research Article

α-glucosidase Inhibitory Activity of Extracts and Compounds from the Leaves of Ruellia tuberosa L.

Author(s): Le Nguyen Tuong Vi, Nguyen Ngoc Tuan, Quach Tong Hung, Pham Thi Nhat Trinh, Tong Thanh Danh, Ngo Thao Ly, Vo Nguyen Thao and Le Tien Dung*

Volume 12, Issue 5, 2022

Published on: 18 February, 2021

Article ID: e180221191577 Pages: 6

DOI: 10.2174/2210315511666210218214955

Price: $65

Abstract

Background: In recent years, the study of the structure and biological activity of medicinal plants has been particularly important to search for diabetes medicine. Ruellia tuberosa is used to treat various diseases such as diabetes by inhibiting the activity of α-glucosidase.

Objective: In this study, an experiment was designed to isolatedisolate and identifiedidentifyα-glucosidase inhibitory extracts and compounds from Ruellia tuberosa L. through bio-assay guided isolation.

Methods: Dry powder of Ruellia tuberosa L. was extracted with 70% ethanol, followed by liquidliquid partition with n-hexane, ethyl acetate and butanol, respectively. The extracts were evaluated withα-glucosidase inhibition. The potential extracts were isolated to identifynew compounds. The effects of these compounds on the α-glucosidase inhibitory were evaluated.

Results: The a-glucosidase inhibitory activities showed that the n-hexane, ethyl acetate and the butanol extract had the α-glucosidase inhibition with an IC50 of 46.2 ± 0.9, 6.6 ± 2.9 and 8.9 ± 0.9 μg/mL, respectively. From the n-hexane and ethyl acetate extracts, the structures of four compounds were elucidated by NMR spectroscopic method, including lupeol (1), syringaresinol (2), apigenin (3), verbascoside (4). The α-glucosidase inhibitory activities showed that all isolated compounds were more active than the positive control - acarbose with an IC50 of 37.5 ± 0.4; 19.5 ± 0.2; 20.1 ± 0.3; 69.3 ± 0.2 μg/mL, respectively.

Conclusion: The ethyl acetate extract showed strong activity about 19 times more than the positive control - acarbose. The compound syringaresinol (2) was the most powerful α-glucosidase inhibitor. Therefore, Ruellia tuberosa L. contains many compounds that can inhibit α-glucosidase activity.

Keywords: Ruellia tuberosa L., explosive fruit tree, α-glucosidase inhibition, diabetes mellitus, lupeol, syringaresinol, apigenin, verbascoside.

Graphical Abstract
[1]
Baynes, H.W. Classification, Pathophysiology, Diagnosis and Management of Diabetes Mellitus. J. Diabetes Metab., 2015, 06(05), 541.
[2]
Asmat, U.; Abad, K.; Ismail, K. Diabetes mellitus and oxidative stress-A concise review. Saudi Pharm. J., 2016, 24(5), 547-553.
[http://dx.doi.org/10.1016/j.jsps.2015.03.013] [PMID: 27752226]
[3]
Shai, L.J.; Masoko, P.; Mokgotho, M.P.; Magano, S.R.; Mogale, A.M.; Boaduo, N.; Eloff, J.N. Yeast alpha glucosidase inhibitory and antioxidant activities of six medicinal plants collected in halaborwa, South Africa. S. Afr. J. Bot., 2010, 76(3), 465-470.
[http://dx.doi.org/10.1016/j.sajb.2010.03.002]
[4]
Dang, P.H.; Nguyen, H.X.; Nguyen, N.T.; Le, H.N.T.; Nguyen, M.T.T. α-Glucosidase inhibitors from the stems of Embelia ribes. Phytother. Res., 2014, 28(11), 1632-1636.
[http://dx.doi.org/10.1002/ptr.5175] [PMID: 24849650]
[5]
Chen, F.A.; Wu, A.B.; Shieh, P.; Kuo, D.H.; Hsieh, C.Y. Evaluation of the antioxidant activity of Ruellia tuberosa. Food Chem., 2006, 94(1), 14-18.
[http://dx.doi.org/10.1016/j.foodchem.2004.09.046]
[6]
Wagner, H.; Danninger, H.; Iyengar, M.A.; Seligmann, O.; Farkas, L.; Subramanian, S.S.; Nair, A.G.R. [Synthesis of glucuronides in the flavonoid-series. 3. Isolation of apigenin-7- -D-glucuronide from Ruellia tuberosa L. and its synthesis]. Chem. Ber., 1971, 104(9), 2681-2687.
[http://dx.doi.org/10.1002/cber.19711040905] [PMID: 5154279]
[7]
Lans, C.A. Creole Remedies. Case studies of ethnoveterinary medicine in Trinidad and Tobago., 2001,
[8]
Chiu, N.Y.; Chang, K.H. The illustrated medicinal plants of Taiwan. Mingtong Med.J., 1995, 226(1)
[9]
Wiart, C.; Hannah, M.; Yassim, M.; Hamimah, H.; Sulaiman, M. Anti-microbial activity of Ruellia tuberosa L. Am. J. Chin. Med., 2005, 33(4), 683-685.
[PMID: 16173541]
[10]
Chothani, D.L.; Patel, M.; Mishra, S.; Vaghasiya, H. Review on Ruellia tuberosa (Cracker plant). Phcog J., 2010, 2(12), 506-512.
[http://dx.doi.org/10.1016/S0975-3575(10)80040-9]
[11]
Arambewela, L.S.R.; Thambugala, R.; Ratnasooriya, W.D. Gastroprotective activity of Ruellia tuberosa root extract in rats. J. Trop. Med. Plants, 2003, 4(2), 191-194.
[12]
Arun, S.; Giridharan, P.; Suthar, A.; Kulkarni-Almeida, A.; Naik, V.; Velmurugan, R.; Ram, V. Isolation of Tylocrebrine from Ruellia tuberosa through bioassay directed column chromatography and elucidating its anti-cancer and anti-inflammatory potential. Book of Abstracts, 7th Joint Meeting of GA, AFERP, ASP, PSI & SIF, Athens, Greece., 2008.
[13]
Fischer, H.; Jensen, W.; Jensen, S.R.; Nielsen, B.J. Chemotaxonomy of the acanthaceae. Iridoids and quaternary amines. Phytochemistry, 1988, 27(8), 2581-2589.
[http://dx.doi.org/10.1016/0031-9422(88)87029-8]
[14]
Singh, R.S.; Pandey, H.S.; Pandey, R.P.; Singh, B.K. A new triterpenoid from Ruellia tuberosa Linn. Indian J. Chem. B, 2002, 41B, 1754-1756.
[15]
Facundo, V.A.; Pinto, A.C.; Rezende, C.M. Aroma compounds and volatile chemical composition of Ruellia menthoides (Nees) Hiern. Flavour Fragrance J., 2005, 20(1), 93-95.
[http://dx.doi.org/10.1002/ffj.1380]
[16]
Abdullahi, S.M.; Musa, A.M.; Abdullahi, M.I.; Sule, M.I.; Sani, Y.M. Isolation of Lupeol from the Stem-bark of Lonchocarpus sericeus (Papilionaceae). Sch. Acad. J. Biosci, 2013, 1(1), 18-19.
[17]
Gohari, A.R.; Saeidnia, S.; Bayati-Moghadam, M.; Amin, G. Lignans and neolignans from Stelleropsis antoninae. Daru, 2011, 19(1), 74-79.
[PMID: 22615643]
[18]
Van Loo, P.; Bruyn, A.D.; Buděšínský, M. Reinvestigation of the structural assignment of signals in the 1H and 13C-NMR spectra of the flavone apigenin. Magn. Reson. Chem., 1986, 24(10), 879-882.
[http://dx.doi.org/10.1002/mrc.1260241007]
[19]
Akdemir, Z.Ş.; Tatli, I.I.; Bedir, E.; Khan, I.A. Iridoid and Phenylethanoid Glycosides from Verbascum lasianthum. Turk. J. Chem., 2004, 28, 227-234.

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