Generic placeholder image

Current Pharmaceutical Design

Editor-in-Chief

ISSN (Print): 1381-6128
ISSN (Online): 1873-4286

General Research Article

Evaluation of Gastroprotective Activity of Linoleic Acid on Gastric Ulcer in a Mice Model

Author(s): José L.R. Martins*, Dayane M. Silva, Emerson H. Gomes, Samuel A. Fava, Murilo F. Carvalho, Isaac Y.L. Macedo, Eric S. Gil, Paulo C. Ghedini, Fábio F. Rocha, Osmar N. Silva*, James O. Fajemiroye, Emerith M.H. Pinto and Elson A. Costa

Volume 28, Issue 8, 2022

Published on: 08 September, 2020

Page: [655 - 660] Pages: 6

DOI: 10.2174/1381612826666200908144053

Price: $65

Abstract

Background: Gastric ulcer has been a major cause of morbidity and mortality worldwide, and it has been linked to factors such as nutritional deficiency, smoking, stress, and continuous intake of non-steroidal antiinflammatory drugs (NSAIDs). The search for new anti-ulcer therapeutic agents has been the subject of several studies. Recently, the gastroprotective effect of Celtis iguanaea has been reported, with linoleic acid (LA) responsible for many of the therapeutic effects of this medicinal plant.

Aims: This study aims to investigate the gastroprotective activity and the possible mechanisms in which LA may be involved through different experimental assays in mice.

Methods: The gastroprotective activity of LA was evaluated in the ulcer induced by indomethacin, HCl/EtOH, hypothermic-restraint stress and pyloric ligation. For the investigation of gastroprotective mechanisms, the quantification of the volume (mL), pH and total acidity of gastric secretion were considered.

Results: The oral administrations of 25 mg/kg, 50 mg/kg or 100 mg/kg of body weight of LA were capable of protecting the gastric mucosa against HCl/ethanol (10 mL/kg p.o.), and oral/intraduodenal treatment administrations of 50 mg/kg LA showed protection from ulcers induced by indomethacin, hypothermic-restraint stress and pyloric ligation.

Conclusion: The results of this study show the gastroprotective role of LA in gastric mucosal damage induced by all assayed distresses. The observed gastroprotection possibly occurs due to the mediated increase of mucosal defensive factors.

Keywords: Medicinal plant, linoleic acid, gastroprotection, ulcer, gastric mucosal, pyloric ligation.

[1]
Halabi MF, Shakir RM, Bardi DA, et al. Gastroprotective activity of ethyl-4-[(3,5-di-tert-butyl-2-hydroxybenzylidene) amino]benzoate against ethanol-induced gastric mucosal ulcer in rats. PLoS One 2014.
[2]
Burci LM, Pereira IT, da Silva LM, et al. Antiulcer and gastric antisecretory effects of dichloromethane fraction and piplartine obtained from fruits of Piper tuberculatum Jacq. in rats. J Ethnopharmacol 2013; 148(1): 165-74.
[3]
Mota KS, Dias GE, Pinto ME, et al. Flavonoids with gastroprotective activity. Molecules 2009; 14(3): 979-1012.
[4]
Zakaria ZA, Balan T, Suppaiah V, Ahmad S, Jamaludin F. Mechanism(s) of action involved in the gastroprotective activity of Muntingia calabura. J Ethnopharmacol 2014; 151(3): 1184-93.
[5]
de Sousa FB, Martins JL, Florentino IF, et al. Preliminary studies of gastroprotective effect of Celtis iguanaea (Jacq.) Sargent leaves (Ulmaceae). Nat Prod Res 2013; 27(12): 1102-7.
[http://dx.doi.org/10.1080/14786419.2012.698407] [PMID: 22712542]
[6]
Hernandez-Galicia E, Aguilar-Contreras A, Aguilar-Santamaria L, et al. Studies on hypoglycemic activity of Mexican medicinal plants. Proc West Pharmacol Soc 2002; 45: 118-24.
[PMID: 12434552]
[7]
Tene V, Malagon O, Finzi PV, Vidari G, Armijos C, Zaragoza T. An ethnobotanical survey of medicinal plants used in Loja and Zamora-Chinchipe. Ecuador J Ethnopharmacology 2007; 111(1): 63-81.
[8]
Martins JL, Rodrigues OR, de Sousa FB, et al. Medicinal species with gastroprotective activity found in the Brazilian Cerrado. Fundam Clin Pharmacol 2015; 29(3): 238-51.
[http://dx.doi.org/10.1111/fcp.12113] [PMID: 25753027]
[9]
Martins JL, Rodrigues OR, da Silva DM, et al. Mechanisms involved in the gastroprotective activity of Celtis iguanaea (Jacq.) Sargent on gastric lesions in mice. J Ethnopharmacol 2014; 155(3): 1616-24.
[http://dx.doi.org/10.1016/j.jep.2014.08.006] [PMID: 25153020]
[10]
Gonzalez-Reyes C, Marcial-Medina C, Cervantes-Anaya N, Cortes-Reynosa P, Salazar EP. Migration and invasion induced by linoleic acid are mediated through fascin in MDA-MB-231 breast cancer cells. Mol Cell Biochem 2018; 443(1-2): 1-10.
[http://dx.doi.org/10.1007/s11010-017-3205-8] [PMID: 29052029]
[11]
Basiricò L, Morera P, Dipasquale D, Tröscher A, Bernabucci U. Comparison between conjugated linoleic acid and essential fatty acids in preventing oxidative stress in bovine mammary epithelial cells. J Dairy Sci 2017; 100(3): 2299-309.
[http://dx.doi.org/10.3168/jds.2016-11729] [PMID: 28088424]
[12]
Viladomiu M, Hontecillas R, Bassaganya-Riera J. Modulation of inflammation and immunity by dietary conjugated linoleic acid. Eur J Pharmacol 2016; 785: 87-95.
[http://dx.doi.org/10.1016/j.ejphar.2015.03.095] [PMID: 25987426]
[13]
Declair V. The usefulness of topical application of essential fatty acids (EFA) to prevent pressure ulcers. Ostomy Wound Manage 1997; 43(5): 48-52.
[14]
Rojo LE, Villano CM, Joseph G, et al. Wound-healing properties of nut oil from Pouteria lucuma. J Cosmet Dermatol 2010; 9(3): 185-95.
[15]
Bardaa S, Ben Halima N, Aloui F, et al. Oil from pumpkin (Cucurbita pepo L.) seeds: evaluation of its functional properties on wound healing in rats. Lipids Health Dis 2016; 15: 73.
[16]
Cardoso CR, Souza MA, Ferro EA, Favoreto S Jr, Pena JD. Influence of topical administration of n-3 and n-6 essential and n-9 nonessential fatty acids on the healing of cutaneous wounds. Wound Repair Regen 2004; 12(2): 235-43.
[http://dx.doi.org/10.1111/j.1067-1927.2004.012216.x] [PMID: 15086775]
[17]
Robert A, Nezamis JE, Lancaster C, Hanchar AJ. Cytoprotection by prostaglandins in rats. Prevention of gastric necrosis produced by alcohol, HCl, NaOH, hypertonic NaCl, and thermal injury. Gastroenterology 1979; 77(3): 433-43.
[http://dx.doi.org/10.1016/0016-5085(79)90002-7] [PMID: 456839]
[18]
Gamberini MT, Skorupa LA, Souccar C, Lapa AJ. Inhibition of gastric secretion by a water extract from Baccharis triptera, Mart. Mem Inst Oswaldo Cruz 1991; 86(Suppl. 2): 137-9.
[http://dx.doi.org/10.1590/S0074-02761991000600031] [PMID: 1841987]
[19]
Senay EC, Levine RJ. Synergism between cold and restraint for rapid production of stress ulcers in rats. Proc Soc Exp Biol Med 1967; 124(4): 1221-3.
[http://dx.doi.org/10.3181/00379727-124-31970] [PMID: 6024838]
[20]
Corne SJ, Morrissey SM, Woods RJ. Proceedings: A method for the quantitative estimation of gastric barrier mucus. J Physiol 1974; 242(2): 116P-7P.
[PMID: 4142046]
[21]
Gil ES, Couto RO. Flavonoid electrochemistry: a review on the electroanalytical applications. Rev Bras Farmacogn 2013; 23(3): 542-58.
[http://dx.doi.org/10.1590/S0102-695X2013005000031]
[22]
Martins JLR, Fajemiroye JO, Hungria EM, et al. Gastroprotective effect of the aqueous fraction of hydroacetonic leaf extract of Eugenia uniflora L. (Myrtaceae) (pitanga) against several gastric ulcer models in mice. J Med Plants Res 2017; 11(39): 603-12.
[http://dx.doi.org/10.5897/JMPR2017.6436]
[23]
Hiruma-Lima CA, Batista LM, de Almeida AB, et al. Antiulcerogenic action of ethanolic extract of the resin from Virola surinamensis Warb. (Myristicaceae). J Ethnopharmacol 2009; 122(2): 406-9.
[http://dx.doi.org/10.1016/j.jep.2008.12.023] [PMID: 19162155]
[24]
Wang Y, Wang SL, Zhang JY, et al. Anti-ulcer and anti-Helicobacter pylori potentials of the ethyl acetate fraction of Physalis alkekengi L. var. franchetii (Solanaceae) in rodent. J Ethnopharmacol 2018; 211: 197-206.
[http://dx.doi.org/10.1016/j.jep.2017.09.004] [PMID: 28964871]
[25]
Santin JR, Lemos M, Klein Júnior LC, Niero R, de Andrade SF. Antiulcer effects of Achyrocline satureoides (Lam.) DC (Asteraceae) (Marcela), a folk medicine plant, in different experimental models. J Ethnopharmacol 2010; 130(2): 334-9.
[http://dx.doi.org/10.1016/j.jep.2010.05.014] [PMID: 20546870]
[26]
Almeida ESD, Cechinel V, Niero R, Clasen BK, Balogun SO, Martins DTD. Pharmacological mechanisms underlying the anti-ulcer activity of methanol extract and canthin-6-one of Simaba ferruginea A. St-Hil. in animal models. J Ethnopharmacology 2011.
[27]
Sepulveda B, Quispe C, Simirgiotis M, et al. Gastroprotective activity of synthetic coumarins: Role of endogenous prostaglandins, nitric oxide, non-protein sulfhydryls and vanilloid receptors. Bioorg Med Chem Lett 2016; 26(23): 5732-5.
[http://dx.doi.org/10.1016/j.bmcl.2016.10.056] [PMID: 27810240]
[28]
Shirwaikar A, Bhilegaonkar PM, Malini S, Kumar JS. The gastroprotective activity of the ethanol extract of Ageratum conyzoides. J Ethnopharmacol 2003; 86(1): 117-21.
[http://dx.doi.org/10.1016/S0378-8741(03)00050-3] [PMID: 12686450]
[29]
Viana AF, Fernandes HB, Silva FV, et al. Gastroprotective activity of Cenostigma macrophyllum Tul. var. acuminata Teles Freire leaves on experimental ulcer models. J Ethnopharmacol 2013; 150(1): 316-23.
[http://dx.doi.org/10.1016/j.jep.2013.08.047] [PMID: 24035848]
[30]
Jawanjal H, Rajput MS, Agrawal P, Dange V. Pharmacological evaluation of fruits of Terminalia belerica Roxb. for antiulcer activity. J Complement Integr Med 2012; 9: 9.
[http://dx.doi.org/10.1515/1553-3840.1556] [PMID: 22713279]
[31]
Laloo D, Prasad SK, Krishnamurthy S, Hemalatha S. Gastroprotective activity of ethanolic root extract of Potentilla fulgens Wall. ex Hook. J Ethnopharmacol 2013; 146(2): 505-14.
[http://dx.doi.org/10.1016/j.jep.2013.01.015] [PMID: 23384786]
[32]
Dias PC, Foglio MA, Possenti A, Nogueira DC, de Carvalho JE. Antiulcerogenic activity of crude ethanol extract and some fractions obtained from aerial parts of Artemisia annua L. Phytother Res 2001; 15(8): 670-5.
[http://dx.doi.org/10.1002/ptr.758] [PMID: 11746858]

Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy