Login Register Cart 0
Generic placeholder image

Anti-Cancer Agents in Medicinal Chemistry

Editor-in-Chief

ISSN (Print): 1871-5206
ISSN (Online): 1875-5992

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Erinacerins, Novel Glioma Inhibitors from Hericium erinaceus, Induce Apoptosis of U87 Cells through Bax/Capase-2 Pathway

Author(s): Feng Zhang, Hui Lv* and Xuhua Zhang*

Volume 20, Issue 17, 2020

Page: [2082 - 2088] Pages: 7

DOI: 10.2174/1871520620666200804104243

Price: $65

Abstract

Background: Glioma is the most common tumor of the central nervous system. Hericium erinaceus, which has been reported to have a variety of pharmacological activities, is a widely used Traditional Chinese Medicine (TCM), and also a kind of delicious food accepted by the public.

Methods and Results: In this study, two new natural products, compounds 1 and 2, were isolated and identified from Hericium erinaceus. They were named erinacerin O and erinacerin P, respectively, after the structural identification, and their effects on human glioma cell line U87 were evaluated. Erinacerin P (2) exhibited obvious cytotoxicity on human glioma cell line U87. The IC50 value of 2 was 19.32μg/mL. The results showed that the apoptosis of U87 cells treated with 2 increased and the morphology of U87 cells altered significantly. Flow cytometry experiment showed that 2 could significantly increase the apoptosis rate of U87 cells and reduce DNA replication. Western blot results suggested the Bax/capase-3 pathway was involved in the U87 cell apoptosis induced by 2.

Conclusion: Erinacerin O and Erinacerin P are novel compounds obtained from Hericium erinaceus and Erinacerin P could be a potential novel glioma inhibitor.

Keywords: Hericium erinaceus, erinacerin, U87, cell viability, apoptosis, nuclear magnetic resonance.

« Previous Next »
Graphical Abstract

[1]
Wang, X.L.; Chi, Y.F.; Xia, H.Q.; Wang, X.J.; Zhao, Y.Y.; Bu, P. Expression and correlation of tensin analogue P53 MGMT Ki-67 in glioma. Zhongguo Yaowu Yu Linchuang, 2015, 15(7), 940-942.
[2]
Yao, W.M.; Piao, X.Y. Progress in glioma. J. Clin. Oncol., 2013, 31(24), 11691-11694.
[3]
Itagaki, T.; Motoyoshi, N.; Kobayashi, H.; Ogawa, Y.; Hirose, D.; Inokuchi, N. Cloning and characterization of ribonuclease T2 gene (RNHe30) from the basidiomycete, Hericium erinaceum. Mycoscience, 2013, 54(3), 188-197.
[http://dx.doi.org/10.1016/j.myc.2012.09.011]
[4]
Friedman, M. Chemistry, nutrition, and health-promoting properties of Hericium erinaceus (Lion’s Mane) mushroom fruiting bodies and mycelia and their bioactive compounds. J. Agric. Food Chem., 2015, 63(32), 7108-7123.
[http://dx.doi.org/10.1021/acs.jafc.5b02914] [PMID: 26244378]
[5]
Miyazawa, M.; Takahashi, T.; Horibe, I.; Ishikawa, R. Two new aromatic compounds and a new d-arabinitol ester from the mushroom Hericium erinaceum. Tetrahedron, 2019, 68(19), 2007-2010.
[6]
Chau, C.F.; Wu, S.H. The development of regulations of Chinese herbal medicines for both medicinal and food uses. Trends Food Sci. Technol., 2006, 17(6), 313-323.
[http://dx.doi.org/10.1016/j.tifs.2005.12.005]
[7]
Wang, J.C.; Hu, S.H.; Lee, W.L.; Tsai, L.Y. Antimutagenicity of extracts of Hericium erinaceus. Kaohsiung J. Med. Sci., 2001, 17(5), 230-238.
[PMID: 11517861]
[8]
Lee, J.S.; Hong, E.K. Hericium erinaceus enhances doxorubicin-induced apoptosis in human hepatocellular carcinoma cells. Cancer Lett., 2010, 297(2), 144-154.
[http://dx.doi.org/10.1016/j.canlet.2010.05.006] [PMID: 20554107]
[9]
Zan, X.; Cui, F.; Li, Y.; Yang, Y.; Wu, D.; Sun, W.; Ping, L. Hericium erinaceus polysaccharide-protein HEG-5 inhibits SGC-7901 cell growth via cell cycle arrest and apoptosis. Int. J. Biol. Macromol., 2015, 76, 242-253.
[http://dx.doi.org/10.1016/j.ijbiomac.2015.01.060] [PMID: 25703932]
[10]
Lee, J.S.; Min, K.M.; Cho, J.Y.; Hong, E.K. Study of macrophage activation and structural characteristics of purified polysaccharides from the fruiting body of Hericium erinaceus. J. Microbiol. Biotechnol., 2009, 19(9), 951-959.
[http://dx.doi.org/10.4014/jmb.0901.013] [PMID: 19809252]
[11]
Kolotushkina, E.V.; Moldavan, M.G.; Voronin, K.Y.; Skibo, G.G. The influence of Hericium erinaceus extract on myelination process in vitro. Fiziol. Zh., 2003, 49(1), 38-45.
[PMID: 12675022]
[12]
Mori, K.; Inatomi, S.; Ouchi, K.; Azumi, Y.; Tuchida, T. Improving effects of the mushroom Yamabushitake (Hericium erinaceus) on mild cognitive impairment: A double-blind placebo-controlled clinical trial. Phytother. Res., 2009, 23(3), 367-372.
[http://dx.doi.org/10.1002/ptr.2634] [PMID: 18844328]
[13]
Zhao, B. Natural antioxidants protect neurons in Alzheimer’s disease and Parkinson’s disease. Neurochem. Res., 2009, 34(4), 630-638.
[http://dx.doi.org/10.1007/s11064-008-9900-9] [PMID: 19125328]
[14]
Nagano, M.; Shimizu, K.; Kondo, R.; Hayashi, C.; Sato, D.; Kitagawa, K.; Ohnuki, K. Reduction of depression and anxiety by 4 weeks Hericium erinaceus intake. Biomed. Res., 2010, 31(4), 231-237.
[http://dx.doi.org/10.2220/biomedres.31.231] [PMID: 20834180]
[15]
Wong, K.H.; Naidu, M.; David, P.; Abdulla, M.A.; Abdullah, N.; Kuppusamy, U.R.; Sabaratnam, V. Peripheral nerve regeneration following crush injury to rat peroneal nerve by aqueous extract of medicinal mushroom Hericium erinaceus (Bull.: Fr) Pers. (Aphyllophoromycetideae). Evid.-. Based Compl. Alt. Med., 2011, 2011580752
[16]
Han, Z.H.; Ye, J.M.; Wang, G.F. Evaluation of in vivo antioxidant activity of Hericium erinaceus polysaccharides. Int. J. Biol. Macromol., 2013, 52, 66-71.
[http://dx.doi.org/10.1016/j.ijbiomac.2012.09.009] [PMID: 23000690]
[17]
Wang, M.; Konishi, T.; Gao, Y.; Xu, D.; Gao, Q. Anti-gastric ulcer activity of polysaccharide fraction isolated from Mycelium culture of Lion’s Mane Medicinal Mushroom, Hericium erinaceus (higher Basidiomycetes). Int. J. Med. Mushrooms, 2015, 17(11), 1055-1060.
[http://dx.doi.org/10.1615/IntJMedMushrooms.v17.i11.50] [PMID: 26853960 ]
[18]
Yang, B.K.; Park, J.B.; Song, C.H. Hypolipidemic effect of an Exo-biopolymer produced from a submerged mycelial culture of Hericium erinaceus. Biosci. Biotechnol. Biochem., 2003, 67(6), 1292-1298.
[http://dx.doi.org/10.1271/bbb.67.1292] [PMID: 12843656]
[19]
Hiwatashi, K.; Kosaka, Y.; Suzuki, N.; Hata, K.; Mukaiyama, T.; Sakamoto, K.; Shirakawa, H.; Komai, M. Yamabushitake mushroom (Hericium erinaceus) improved lipid metabolism in mice fed a high-fat diet. Biosci. Biotechnol. Biochem., 2010, 74(7), 1447-1451.
[http://dx.doi.org/10.1271/bbb.100130] [PMID: 20622452]
[20]
Wang, X.L.; Xu, K.P.; Long, H.P.; Zou, H.; Cao, X.Z.; Zhang, K.; Hu, J.Z.; He, S.J.; Zhu, G.Z.; He, X.A.; Xu, P.S.; Tan, G.S. New isoindolinones from the fruiting bodies of Hericium erinaceum. Fitoterapia, 2016, 111, 58-65.
[http://dx.doi.org/10.1016/j.fitote.2016.04.010] [PMID: 27094113]
[21]
Nozawa, Y.; Ito, M.; Sugawara, K.; Hanada, K.; Mizoue, K. Stachybotrin C and parvisporin, novel neuritogenic compounds. II. Structure determination. J. Antibiot. (Tokyo), 1997, 50(8), 641-645.
[http://dx.doi.org/10.7164/antibiotics.50.641] [PMID: 9315075]

Rights & Permissions Print Cite
Article Metrics
42
© 2024 Bentham Science Publishers | Privacy Policy