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Endocrine, Metabolic & Immune Disorders - Drug Targets

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ISSN (Print): 1871-5303
ISSN (Online): 2212-3873

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General Research Article

Ruta Montana Evokes Antihypertensive Activity Through an Increase of Prostaglandins Release in L-NAME-Induced Hypertensive Rats

Author(s): Fadwa El-Ouady and Mohamed Eddouks*

Volume 21, Issue 2, 2021

Published on: 27 June, 2020

Page: [305 - 314] Pages: 10

DOI: 10.2174/1871530320666200628025430

Price: $65

Abstract

Aims: The aim of the study was to experimentally investigate the antihypertensive effect of Ruta Montana.

Background: Ruta montana L. is traditionally used in Moroccan herbal medicine to treat hypertension. This study aimed to experimentally evaluate the hypotensive and vasoactive properties of this plant.

Objective: The objective of the study was to evaluate the effect of the aqueous extract of Ruta Montana on blood pressure parameters in LNAME-induced hypertensive rats and to determine the vasorelaxant activity of this aqueous extract.

Methods: The antihypertensive effect of the aqueous extract obtained from Ruta montana aerial parts (RMAPAE) (200 mg/kg) was evaluated in normal and anesthetized hypertensive rats. Blood pressure parameters (systolic blood pressure (SBP), mean blood pressure (MBP) and diastolic blood pressure (DBP)) and heart rate were measured using a tail-cuff and a computer-assisted monitoring device. The acute and chronic effect of RMAPAE was recorded for 6 hours for the acute experiment and for 7 days for the sub-chronic test. In the other set, the vasorelaxant effect of RMAPAE on the contractile response was observed in the isolated thoracic aorta.

Results: The results indicated that the RMAPAE extract significantly decreased SBP, MBP, DBP and heart rate in L-NAME-induced hypertensive rats. Furthermore, RMAPAE was demonstrated to induce a dose-dependent relaxation in the aorta precontracted with Epinephrine or KCl. More interestingly, this vasorelaxant activity of RMAPAE seems to be probably mediated through the prostaglandins pathway.

Conclusion: The present study illustrates the beneficial action of Ruta montana on hypertension and supports its use as an antihypertensive agent.

Keywords: Ruta montana, hypertension, vasorelaxant, prostaglandins, LNAME-hypertensive rats, isolated aortic ring.

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[1]
Veeramani, C.; Al-Numair, K.S.; Chandramohan, G.; Alsaif, M.A.; Alhamdan, A.A.; Pugalendi, K.V. Antihypertensive effect of Melothria maderaspatana leaf fractions on DOCA-salt-induced hypertensive rats and identification of compounds by GC-MS analysis. J. Nat. Med., 2012, 66(2), 302-310.
[http://dx.doi.org/10.1007/s11418-011-0590-2] [PMID: 21964566]
[2]
Singh, P.; Mishra, A.; Singh, P. Hypertension and herbal plant for its treatment: a review Ind. J. Res. Pharm. Biotech, 2015, 2320-3471.
[3]
Al Disi, S.S.; Anwar, M.A.; Eid, A.H. Anti-hypertensive herbs and their mechanisms of action: part I. Front. Pharmacol., 2016, 6, 323.
[http://dx.doi.org/10.3389/fphar.2015.00323] [PMID: 26834637]
[4]
Djarri, L.; Ferhat, M.; Merabet, G.; Chelghoum, A.; Laggoune, S.; Semra, Z.; Smati, F.; Kabouche, Z. Composition and antibacterial activity of the essential oil of Ruta montana from Constantine (Algeria). Der Pharm. Lett., 2013, 5(4), 70-73.
[5]
Touati, D. Rahman, A.u.; Ulubelen, A. Alkaloids from Ruta montana. Phytochemistry, 2000, 53(2), 277-279.
[http://dx.doi.org/10.1016/S0031-9422(99)00486-0] [PMID: 10680183]
[6]
Baba iassa F. Encyclopédie des Plantes Utiles: Flore d’Algérie et du Maghreb. Ed : Librairie Moderne –Rouiba, 1999; pp. 243-244.
[7]
Benziane, M.M. Screening photochimique de la plante Ruta montana. Extraction de l’huile essentielle et de la rutine., Activité antioxydant de la plante. PhD Thesis, Université D'oran Es-sénia: Morocco, 2007.
[8]
Lahsissene, H.; Kahouadji, A.; Tijane, M.; Hseini, S. Meidicinal plants used in the region of Zaer (Occidental Morocco) Be-issn, 2009, 0457-4184.
[9]
Benítez, G.; González-Tejero, M.R.; Molero-Mesa, J. Pharmaceutical ethnobotany in the western part of Granada province (southern Spain): ethnopharmacological synthesis. J. Ethnopharmacol., 2010, 129(1), 87-105.
[http://dx.doi.org/10.1016/j.jep.2010.02.016] [PMID: 20226847]
[10]
Hammami, I.; Smaoui, S.; Hsouna, A.B.; Hamdi, N.; Triki, M.A. Ruta montana L. leaf essential oil and extracts: characterization of bioactive compounds and suppression of crown gall disease. EXCLI J., 2015, 14, 83-94.
[PMID: 26417353]
[11]
Hammiche, V.; Azzouz, M. Ruta. Ethnobotany, phytopharmacology and toxicity. Phytotherapie, 2013, 11(1), 22-30.
[http://dx.doi.org/10.1007/s10298-013-0751-9]
[12]
Duke, A.J.; Duke, P.A.K.; Ducellie, J.L. Duke’s handbook of medicinal plants of the bible; CRC Press: Florida, 2008, pp. 394-398.
[13]
Jouad, H.; Haloui, M.; Rhiouani, H.; El Hilaly, J.; Eddouks, M. Ethnobotanical survey of medicinal plants used for the treatment of diabetes, cardiac and renal diseases in the North centre region of Morocco (Fez-Boulemane). J. Ethnopharmacol., 2001, 77(2-3), 175-182.
[http://dx.doi.org/10.1016/S0378-8741(01)00289-6] [PMID: 11535361]
[14]
Eddouks, M.; Maghrani, M.; Lemhadri, A.; Ouahidi, M.L.; Jouad, H. Ethnopharmacological survey of medicinal plants used for the treatment of diabetes mellitus, hypertension and cardiac diseases in the south-east region of Morocco (Tafilalet). J. Ethnopharmacol., 2002, 82(2-3), 97-103.
[http://dx.doi.org/10.1016/S0378-8741(02)00164-2] [PMID: 12241983]
[15]
Ajebli, M.; Eddouks, M. Antihypertensive activity of Petroselinum crispum through inhibition of vascular calcium channels in rats. J. Ethnopharmacol., 2019, 242112039
[http://dx.doi.org/10.1016/j.jep.2019.112039] [PMID: 31252093]
[16]
El-Ouady, F.; Eddouks, M. Warionia saharae induces antihypertensive and vasorelaxant activities through nitric oxide and KATP channels pathways in rats. J. Complement. Integr. Med., 2019, 17(1) /j/jcim.2019.17.issue-1/jcim-2019-0024/jcim-2019-0024.xml.
[http://dx.doi.org/10.1515/jcim-2019-0024] [PMID: 31348761]
[17]
Aekthammarat, D.; Pannangpetch, P.; Tangsucharit, P. Moringa oleifera leaf extract induces vasorelaxation via endothelium-dependent hyperpolarization and calcium channel blockade in mesenteric arterial beds isolated from L-NAME hypertensive rats. Clin. Exp. Hypertens., 2020, 42(6), 490-501.
[http://dx.doi.org/10.1080/10641963.2020.1714640] [PMID: 31965874]
[18]
Aekthammarat, D.; Pannangpetch, P.; Tangsucharit, P. Moringa oleifera leaf extract lowers high blood pressure by alleviating vascular dysfunction and decreasing oxidative stress in L-NAME hypertensive rats. Phytomedicine, 2019, 54, 9-16.
[http://dx.doi.org/10.1016/j.phymed.2018.10.023] [PMID: 30668387]
[19]
Amssayef, A.; Eddouks, M. Aqueous extract of Matricaria pubescens exhibits antihypertensive activity in L-NAME-induced hypertensive rats through its vasorelaxant effect. Cardiovasc. Hematol. Agents Med. Chem., 2019, 17(2), 135-143.
[http://dx.doi.org/10.2174/1871525717666191007151413] [PMID: 31589128]
[20]
Salami, S.A.; Salahdeen, H.M.; Ugbebor, E.C.; Murtala, B.A.; Raji, Y. Effects of aqueous leaf extract of Tridax procumbens on contractile activity of corpus cavernosum in N-nitro-l-arginine methyl ester-induced hypertensive male rats. J. Integr. Med., 2018, 16(1), 51-56.
[http://dx.doi.org/10.1016/j.joim.2017.11.001] [PMID: 29397093]
[21]
Dib, I.; Tits, M.; Angenot, L.; Wauters, J.N.; Assaidi, A.; Mekhfi, H.; Aziz, M.; Bnouham, M.; Legssyer, A.; Frederich, M.; Ziyyat, A. Antihypertensive and vasorelaxant effects of aqueous extract of Artemisia campestris L. from Eastern Morocco. J. Ethnopharmacol., 2017, 206, 224-235.
[http://dx.doi.org/10.1016/j.jep.2017.05.036] [PMID: 28578165]
[22]
Fink, J.; Fan, N.Y.; Rosenfeld, L.; Stier, C.T., Jr Contribution of endothelin to the acute pressor response of L-NAME in stroke-prone spontaneously hypertensive rats. J. Cardiovasc. Pharmacol., 1998, 31(4), 618-622.
[http://dx.doi.org/10.1097/00005344-199804000-00022] [PMID: 9554813]
[23]
Kopincová, J.; Púzserová, A.; Bernátová, I. L-NAME in the cardiovascular system - nitric oxide synthase activator? Pharmacol. Rep., 2012, 64(3), 511-520.
[http://dx.doi.org/10.1016/S1734-1140(12)70846-0] [PMID: 22814004]
[24]
Kostis, J.B.; Davis, B.R.; Cutler, J.; Grimm, R.H., Jr; Berge, K.G.; Cohen, J.D.; Lacy, C.R.; Perry, H.M., Jr; Blaufox, M.D.; Wassertheil-Smoller, S.; Black, H.R.; Schron, E.; Berkson, D.M.; Curb, J.D.; Smith, W.M.; McDonald, R.; Applegate, W.B. SHEP Cooperative research group. Prevention of heart failure by antihypertensive drug treatment in older persons with isolated systolic hypertension. JAMA, 1997, 278(3), 212-216.
[http://dx.doi.org/10.1001/jama.1997.03550030052033] [PMID: 9218667]
[25]
Staessen, J.A.; Thijs, L.; Fagard, R.; O’Brien, E.T.; Clement, D.; de Leeuw, P.W.; Mancia, G.; Nachev, C.; Palatini, P.; Parati, G.; Tuomilehto, J.; Webster, J. Systolic Hypertension in Europe trial investigators. Predicting cardiovascular risk using conventional vs ambulatory blood pressure in older patients with systolic hypertension. JAMA, 1999, 282(6), 539-546.
[http://dx.doi.org/10.1001/jama.282.6.539] [PMID: 10450715]
[26]
Rawat, P.; Singh, P.K.; Vipin, K. Antihypertensive medicinal plants and their mode of action. J. Herb. Med., 2016.
[http://dx.doi.org/10.1016/j.hermed.2016.06.001]
[27]
Török, J. Histamine-induced relaxation in pulmonary artery of normotensive and hypertensive rats: relative contribution of prostanoids, nitric oxide and hyperpolarization. Physiol. Res., 2000, 49(1), 107-114.
[PMID: 10805411]
[28]
Luksha, L.; Agewall, S.; Kublickiene, K. Endothelium-derived hyperpolarizing factor in vascular physiology and cardiovascular disease. Atherosclerosis, 2009, 202(2), 330-344.
[http://dx.doi.org/10.1016/j.atherosclerosis.2008.06.008] [PMID: 18656197]
[29]
Jaffe, E.A.; Levin, R.I.; Weksler, B.B.; Marcus, A.J. Synthesis of prostacyclin by cultured endothelial cells. Pathobiology of Endothelial Cells; Nossel, H.L; Vogel, H.J., Ed.; , 1982, pp. 301-309.
[http://dx.doi.org/10.1016/B978-0-12-521980-8.50024-9]
[30]
Patrono, C.; Dunn, M.J. The clinical significance of inhibition of renal prostaglandin synthesis. Kidney Int., 1987, 32(1), 1-12.
[http://dx.doi.org/10.1038/ki.1987.164] [PMID: 3306093]
[31]
Indomethacin capsule; Eatontown, NJ: Heritage Pharmaceuticals, Inc, 2014.
[32]
Tolman, E.L.; Rosenthale, M.E.; Capetola, R.J.; McGuire, J.L. Suprofen: the pharmacology and clinical efficacy of a new non-narcotic peripheral analgesic. Clin. Rheum. Dis., 1984, 10(2), 353-368.
[PMID: 6439459]
[33]
Zhang, Y.; Guan, Y.; Schneider, A.; Brandon, S.; Breyer, R.M.; Breyer, M.D. Characterization of murine vasopressor and vasodepressor prostaglandin E(2) receptors. Hypertension, 2000, 35(5), 1129-1134.
[http://dx.doi.org/10.1161/01.HYP.35.5.1129] [PMID: 10818076]
[34]
Jia, Z.; Aoyagi, T.; Yang, T. mPGES-1 protects against DOCA-salt hypertension via inhibition of oxidative stress or stimulation of NO/cGMP. Hypertension, 2010, 55(2), 539-546.
[http://dx.doi.org/10.1161/HYPERTENSIONAHA.109.144840] [PMID: 20065149]
[35]
Tilley, S.L.; Audoly, L.P.; Hicks, E.H.; Kim, H.S.; Flannery, P.J.; Coffman, T.M.; Koller, B.H. Reproductive failure and reduced blood pressure in mice lacking the EP2 prostaglandin E2 receptor. J. Clin. Invest., 1999, 103(11), 1539-1545.
[http://dx.doi.org/10.1172/JCI6579] [PMID: 10359563]
[36]
Jia, Z.; Guo, X.; Zhang, H.; Wang, M.H.; Dong, Z.; Yang, T. Microsomal prostaglandin synthase-1-derived prostaglandin E2 protects against angiotensin II-induced hypertension via inhibition of oxidative stress. Hypertension, 2008, 52(5), 952-959.
[http://dx.doi.org/10.1161/HYPERTENSIONAHA.108.111229] [PMID: 18824665]
[37]
Vasudevan, T.N.; Luckner, M. Alkaloids from Ruta angustifolia Pers., Ruta chalepensis L., Ruta graveolens L. and Ruta montana Mill. Pharmazie, 1968, 23(9), 520-521.
[PMID: 5717194]
[38]
Ulubele, A. A new alkaloid, montanine from Ruta montana. J. Nat. Prod., 1990, 53, 207-208.
[http://dx.doi.org/10.1021/np50067a034]
[39]
Djeridane, A.; Yousf, M.; Nadjemi, B.; Boutassouna, D.; Stocker, P.; Vidal, N. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chem., 2006, 97, 654-660.
[http://dx.doi.org/10.1016/j.foodchem.2005.04.028]
[40]
Nielsen, B.E.; Lemmich, J. Constituents of Umbelliferous plants. Acta Chem. Scand., 1969, 23, 962.
[http://dx.doi.org/10.3891/acta.chem.scand.23-0962] [PMID: 5806314]
[41]
Caldwell, AG.; Jones, ER. 1945, The constituents of expressed West Indian Lime oil Chem Sw, 1, 540-543.
[42]
Angaji, S.A.; Mousavi, S.F.; Babapour, E. Antioxidants: a few key points. Ann. Biol. Res., 2012, 3, 3968-3977.
[43]
Chan, E.C.; Pannangpetch, P.; Woodman, O.L. Relaxation to flavones and flavonols in rat isolated thoracic aorta: mechanism of action and structure-activity relationships. J. Cardiovasc. Pharmacol., 2000, 35(2), 326-333.
[http://dx.doi.org/10.1097/00005344-200002000-00023] [PMID: 10672869]
[44]
Schmitt, C.A.; Dirsch, V.M. Modulation of endothelial nitric oxide by plant-derived products. Nitric Oxide, 2009, 21(2), 77-91.
[http://dx.doi.org/10.1016/j.niox.2009.05.006] [PMID: 19497380]

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