Login Register Cart 0
Generic placeholder image

Current Nutrition & Food Science

Editor-in-Chief

ISSN (Print): 1573-4013
ISSN (Online): 2212-3881

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Microwave-Assisted Extraction of Andrographolide, 14-Deoxy-11,12- didehydroandrographolide and Neoandrographolide from Andrographis paniculata: An Optimisation Study

Author(s): Vi V. Chia, Sook F. Pang, Sureena Abdullah, Mashitah M. Yusoff and Jolius Gimbun*

Volume 16, Issue 9, 2020

Page: [1318 - 1325] Pages: 8

DOI: 10.2174/1573401316666200228120300

Price: $65

Abstract

Background: Andrographis paniculata is often used as functional foods owing to its broad range of pharmaceutical activities due to the presence of main terpene compounds such as andrographolide, 14-deoxy-11,12-didehydroandrographolide and neoandrographolide. The aforementioned compounds must be extracted from the plant material before it can be routinely used as a functional food ingredient.

Objective: This work presents an optimisation study of microwave-assisted extraction (MAE) of terpene from Andrographis paniculata. In this study, two factors at three-level central composite face centred design was employed to optimise the process variables, including the effect of microwave power (75-175 W) and ethanol concentration (20-85%) on extraction of terpene from Andrographis paniculata.

Methods: The experimental design was set based on initial screening on the effect of mesh size and solid solvent ratio. The present work was performed at fixed mesh size and solid solvent ratio, which corresponds to the highest yield established experimentally. Two most significant factors, i.e. microwave power and ethanol concentration was chosen and further studied for optimisation using a response surface methodology. The three-level, two-factor central composite design (CCD) has been employed to determine the effect of microwave power (15, 25 and 35 W/mL) and ethanol concentration (20, 52.5 and 85%) on the yield of terpene extraction. Identification and quantification of the terpene were performed via Ultra-Performance Liquid Chromatography- Photodiode Array (UPLCPDA).

Results: The regression analysis showed good fit of the experimental data to the quadratic equation with coefficient of determination (R2) value of 0.997. The optimum condition was found at microwave power of 140 W with 85% ethanol concentration, produced the highest yield of andrographolide (10.926 ± 0.053 mg/g DW), 14-deoxy-11,12-didehydroandrographolide (4.336 ± 0.215 mg/g DW) and neoandrographolide (5.698 ± 0.252 mg/g DW). The model developed through response surface modelling has a desirability of 97.5% and the error between the experiment and the model predictions ranged from 2.4 to 5.2%.

Conclusion: A successful optimisation of terpene extraction from A. paniculata via MAE was achieved at microwave power of 140 W and 85% ethanol. The model developed from the regression analysis is sufficiently accurate and can be used to predict the yield of terpene extraction from A. paniculata.

Keywords: 14-deoxy-11, 12-didehydroandrographolide, Andrographis paniculata, andrographolide, microwave extraction, neoandrographolide, response surface methodology.

« Previous Next »
Graphical Abstract

[1]
Arifullah M, Namsa ND, Mandal M, Chiruvella KK, Vikrama P, Gopal GR. Evaluation of anti-bacterial and anti-oxidant potential of andrographolide and echiodinin isolated from callus culture of Andrographis paniculata Nees. Asian Pac J Trop Biomed 2013; 3(8): 604-10.
[http://dx.doi.org/10.1016/S2221-1691(13)60123-9 PMID: 23905016]
[2]
Hossain M, Urbi Z, Sule A, Rahman K. Andrographis paniculata (Burm. f.) Wall. ex Nees: a review of ethnobotany, phytochemistry and pharmacology. Sci World J 2014; 2014: 28-57.
[3]
Pholphana N, Panomvana D, Rangkadilok N, et al. A simple and sensitive LC-MS/MS method for determination of four major active diterpenoids from Andrographis paniculata in human plasma and its application to a pilot study. Planta Med 2016; 82(1-2): 113-20.
[PMID: 26576030]
[4]
Zhang XF, Tan BK. Antihyperglycaemic and anti-oxidant properties of Andrographis paniculata in normal and diabetic rats. Clin Exp Pharmacol Physiol [Internet] 2000; 27(5-6): 358-63.http://www.ncbi.nlm.nih.gov/pubmed/10831236
[http://dx.doi.org/10.1046/j.1440-1681.2000.03253.x] [PMID: 10831236]
[5]
Wu TS, Chern HJ, Damu AG, et al. Flavonoids and ent-labdane diterpenoids from Andrographis paniculata and their antiplatelet aggregatory and vasorelaxing effects. J Asian Nat Prod Res 2008; 10(1-2): 17-24.
[http://dx.doi.org/10.1080/10286020701273627] [PMID: 18058376]
[6]
Chen LX, He H, Xia GY, Zhou KL, Qiu F. A new flavonoid from the aerial parts of Andrographis paniculata. Nat Prod Res [Internet] 2014; 28(3): 138-43.
[http://dx.doi.org/10.1080/14786419.2013.856907] [PMID: 24236635]
[7]
Chen K, Zhang X, Pan J, Yin W. Recrystallization of andrographolide using the supercritical fluid antisolvent process. J Cryst Growth 2005; 274(1-2): 226-32.
[http://dx.doi.org/10.1016/j.jcrysgro.2004.09.089]
[8]
Song YX, Liu SP, Jin Z, Qin JF, Jiang ZY. Qualitative and quantitative analysis of Andrographis paniculata by rapid resolution liquid chromatography/time-of-flight mass spectrometry. Molecules 2013; 18(10): 12192-207.
[http://dx.doi.org/10.3390/molecules181012192] [PMID: 24084022]
[9]
Vasu S, Palaniyappan V, Badami S. A novel microwave-assisted extraction for the isolation of andrographolide from Andrographis paniculata and its in vitro antioxidant activity. Nat Prod Res 2010; 24(16): 1560-7.
[http://dx.doi.org/10.1080/14786419.2010.495071] [PMID: 20835957]
[10]
Cui Y, Wang Y, Ouyang X, Han Y, Zhu H, Chen Q. Fingerprint profile of active components for Andrographis paniculata Nees by HPLC-DAD. Sens Instrum Food Qual Saf 2009; 3(3): 165-79.
[http://dx.doi.org/10.1007/s11694-009-9082-4]
[11]
Benattia FK, Arrar Z. Antioxidative and antiradical activities of bioactive compounds of extracts from Algerian prickly pear (Opuntia ficus-indica L) fruits. Curr Nutr Food Sci 2018; 14(3): 211-7.
[http://dx.doi.org/10.2174/1573401313666170609101639]
[12]
Bhan M, Satija S, Garg C, Dureja H, Garg M. Optimization of ionic liquid-based microwave assisted extraction of a diterpenoid lactone-andrographolide from Andrographis paniculata by response surface methodology. J Mol Liq [Internet] 2017; 229: 161-6.
[http://dx.doi.org/10.1016/j.molliq.2016.12.011]
[13]
Karioti A, Timoteo P, Bergonzi MC, Bilia AR. A validated method for the quality control of Andrographis paniculata preparations. Planta Med 2017; 83(14-15): 1207-13.
[http://dx.doi.org/10.1055/s-0043-113827] [PMID: 28651289]
[14]
Chia V, Pang S, Abdullah S, Yusoff M. Effect of amplitude on ultrasonic assisted extraction of caffeic acid from Andrographis paniculata. Int J Eng Adv Technol 2019; 8(3): 524-7.
[15]
Farahmandfar R, Asnaashari M, Asadi Y, Beyranvand B. Comparison of bioactive compounds of Matricaria recutita extracted by ultrasound and maceration and their effects on preventing sunflower oil during frying. Curr Nutr Food Sci 2019; 15(2): 156-64.
[http://dx.doi.org/10.2174/1573401313666170712110248]
[16]
Mayengbam S, Khattab R, Thiyam-Hollander U. Effect of conventional and microwave toasting on sinapic acid derivatives and canolol content of canola. Curr Nutr Food Sci 2013; 9(4): 321-7.
[http://dx.doi.org/10.2174/157340130904131122094946]
[17]
Sareer O, Ahmad S, Umar S. Andrographis paniculata: a critical appraisal of extraction, isolation and quantification of andrographolide and other active constituents. Nat Prod Res [Internet] 2014; 28(23): 2081-101.
[http://dx.doi.org/10.1080/14786419.2014.924004] [PMID: 24912126]
[18]
Lee TY, Lee KC, Chang HH. Modulation of the cannabinoid receptors by andrographolide attenuates hepatic apoptosis following bile duct ligation in rats with fibrosis. Apoptosis 2010; 15(8): 904-14.
[http://dx.doi.org/10.1007/s10495-010-0502-z] [PMID: 20446039]
[19]
Pholphana N, Rangkadilok N, Thongnest S, Ruchirawat S, Ruchirawat M, Satayavivad J. Determination and variation of three active diterpenoids in Andrographis paniculata (Burm.f.) Nees. Phytochem Anal 2004; 15(6): 365-71.
[http://dx.doi.org/10.1002/pca.789] [PMID: 15595451]

Rights & Permissions Print Cite
Article Metrics
23
© 2024 Bentham Science Publishers | Privacy Policy