Evaluation of Diuretic and Antioxidant Properties in Aqueous Bark and Fruit Extracts of Pine

Title:Evaluation of Diuretic and Antioxidant Properties in Aqueous Bark and Fruit Extracts of Pine

Volume: 18 Issue: 1

Author(s): Hadi Shariati, Mohammad Hassanpour, Gholamreza Sharifzadeh, Asghar Zarban*, Saeed Samarghandian and Farhad Saeedi

Affiliation:

• Department of Clinical Biochemistry, Faculty of Medicine, Birjand University of Medical Sciences, Birjand,Iran

Keywords: Antioxidant, diuretics, kidney stones, phenolic content, pine, glomerular filtration rate (GFR).

Abstract:

Objective: The present study has been carried out to evaluate the diuretic and antioxidant properties of pine herb in an animal model.

Materials and Methods: 45 adult male rats were randomly divided into nine groups including: groups I (the negative control), groups II (positive control, furosemide 10 mg/kg), groups III to VIII (treatment groups received 100, 200, 400 mg/kg of the aqueous extracts of bark and fruit) and group IX received the combination of aqueous extract of bark (100 mg/kg) and the fruit (100 mg/kg). The urine output, glomerular filtration rate (GFR), electrolytes, urea, and creatinine levels were evaluated. Furthermore, the phenolic content and antioxidant activity of both extracts were also assessed using 2, 2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and Folin–Ciocalteu methods.

Results: The aqueous extracts of the pine bark and fruit increased the urinary output in a dosedependent manner. The combination of the two extracts compared to the other extracts alone significantly increased the serum potassium level. This study also showed each extract increase creatinine clearance in a dose-dependent manner (p<0.01 and p<0.05). The increase of GFR in the combination group was not significant. The current data showed a significant increase in the total phenolic content in pine bark extract in compared with the fruit extract.

Conclusion: The pine bark and fruit can be useful in the prevention and treatment of kidney stones due to the high diuretic properties and antioxidant activity.

Cite this article as:

Shariati Hadi , Hassanpour Mohammad , Sharifzadeh Gholamreza , Zarban Asghar *, Samarghandian Saeed and Saeedi Farhad , Evaluation of Diuretic and Antioxidant Properties in Aqueous Bark and Fruit Extracts of Pine, Current Drug Discovery Technologies 2021; 18 (1) . https://dx.doi.org/10.2174/1570163817666200206105231