Login Register Cart 0
Generic placeholder image

Endocrine, Metabolic & Immune Disorders - Drug Targets

Editor-in-Chief

ISSN (Print): 1871-5303
ISSN (Online): 2212-3873

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Antiproliferative and Apoptotic Properties of Andrographolide Against Human Colon Cancer DLD1 Cell Line

Author(s): Imran Khan, Sadaf Mahfooz and Irfan A. Ansari*

Volume 20, Issue 6, 2020

Page: [930 - 942] Pages: 13

DOI: 10.2174/1871530319666191125111920

Price: $65

Abstract

Background: In recent years, natural products have received great attention for cancer prevention owing to their various health benefits, noticeable lack of toxicity and side effects, and the limitations of chemotherapeutic agents. Andrographolide, a labdane diterpenoid is a principal bioactive constituent of Andrographis paniculata Nees, exhibits significant anticancer activity.

Objective: The efficacy of andrographolide on colon cancer cells is yet to be elucidated completely. Therefore, we investigated the anticancer efficiency of andrographolide in colon cancer DLD1 cell line.

Methods: Antiproliferative activity of andrographolide on DLD1 cells was evaluated by MTT assay, LDH release assay, morphological analysis and colony formation assay. Induction of apoptosis was determined by DAPI staining, Annexin V-FITC staining assay, and caspase-3 activation assay. Role of andrographolide induced cellular reactive oxygen species (ROS) and its association with apoptosis induction in DLD1 cells was elucidated by DCFDA dye. Synergistic ability of andrographolide with 5- fluorouracil (5-FU) and paclitaxel (PTX) was evaluated by MTT assay.

Results: Results of the present study indicated that andrographolide declined cell viability of DLD1 cells in a concentration and time-dependent manner. Andrographolide induced apoptosis via nuclear condensation, phosphatidylserine externalization and caspase-3 activation. It also augmented cellular ROS levels which were in turn associated with apoptosis induction in DLD1 cells. Moreover, andrographolide displayed synergistic activity with 5-FU and PTX against DLD1 cells.

Conclusion: The present study showed that andrographolide demonstrated antiproliferative and apoptotic properties, moreover it also displayed synergistic effect with chemotherapeutic drugs in colon cancer DLD1 cells.

Keywords: Andrographolide, colon cancer, DLD1, apoptosis, chemopreventive, synergistic.

« Previous Next »
Graphical Abstract

[1]
Ferlay, J.; Soerjomataram, I.; Dikshit, R.; Eser, S.; Mathers, C.; Rebelo, M.; Parkin, D.M.; Forman, D.; Bray, F. Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int. J. Cancer, 2015, 136(5), E359-386.https://onlinelibrary.wiley.com/doi/full/10.1002/ijc.29210
[PMID: 25220842]
[2]
Jemal, A.; Bray, F.; Center, M.M.; Ferlay, J.; Ward, E.; Forman, D. Global cancer statistics. CA Cancer J. Clin., 2011, 61(2), 69-90.https://onlinelibrary.wiley.com/doi/full/10.3322/caac.20107
[PMID: 21296855]
[3]
Center, M.M.; Jemal, A.; Ward, E. International trends in colorectal cancer incidence rates. Cancer Epidemiol. Biomarkers Prev., 2009, 18(6), 1688-1694.https://cebp.aacrjournals.org/content/18/6/1688
[PMID: 19505900]
[4]
Nandakumar, A. National cancer registry programme. J Indian Council of Medical Research, Consolidated report of the population based cancer registries, New Delhi, India,, 1990,, 96..https://mohfw.gov.in/sites/default/files/Cancer%20Registration%20In%20India.pdf
[5]
Norwati, D.; Harmy, M.Y.; Norhayati, M.N.; Amry, A.R. Colorectal cancer screening practices of primary care providers: results of a national survey in Malaysia. J. Asian Pac. J. Cancer Prev., 2014, 15(6), 2901-2904.http://koreascience.or.kr/article/JAKO201418342937408.page
[PMID: 24761922]
[6]
Mokaberi, P.; Reyhani, V.; Amiri-Tehranizadeh, Z.; Saberi, M.R.; Beigoli, S.; Samandar, F.; Chamani, J. New insights into the binding behavior of lomefloxacin and human hemoglobin using biophysical techniques: binary and ternary approaches. New J. Chem., 2019, 43(21), 8132-8145.https://pubs.rsc.org/en/content/articlelanding/2019/nj/c9nj01048c#!divAbstract
[7]
Dehghani Sani, F.; Shakibapour, N.; Beigoli, S.; Sadeghian, H.; Hosainzadeh, M.; Chamani, J. Changes in binding affinity between ofloxacin and calf thymus DNA in the presence of histone H1: Spectroscopic and molecular modeling investigations. J. Lumin., 2018, 203, 599-608.
[http://dx.doi.org/10.1016/j.jlumin.2018.06.083]
[8]
Sharif-Barfeh, Z.; Beigoli, S.; Marouzi, S.; Rad, A.S.; Asoodeh, A.; Chamani, J. Multi-spectroscopic and HPLC studies of the interaction between estradiol and cyclophosphamide with human serum albumin: Binary and ternary systems. J. Solution Chem., 2017, 46(2), 488-504.
[http://dx.doi.org/10.1007/s10953-017-0590-2]
[9]
Hosseini, A.; Ghorbani, A. Cancer therapy with phytochemicals: evidence from clinical studies. Avicenna J. Phytomed.,, 2015,, 5(2), 84-97. 2015,, 5(2) 84..https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4418057
[PMID: 4418057]
[10]
Hosseini, S.; Chamani, J.; Hadipanah, M.R.; Ebadpour, N.; Hojjati, A.S.; Mohammadzadeh, M.H.; Rahimi, H.R. Nano-curcumin's suppression of breast cancer cells (MCF7) through the inhibition of cyclinD1 expression. Breast Cancer (Dove Medical Press), 2019, 11, 137-142.
[http://dx.doi.org/10.2147/BCTT.S195800]] [PMID: 30936742]
[11]
Patel, P.S.; Varney, M.L.; Dave, B.J.; Singh, R.K. Regulation of constitutive and induced NF--κB activation in malignant melanoma cells by capsaicin modulates interleukin-8 production and cell proliferation. J. Interferon Cytokine Res., 2002, 22(4), 427-435.https://link.springer.com/article/10.1007%2Fs00432-002-0368-8
[PMID: 4057320]
[12]
Subbaramaiah, K.; Michaluart, P.; Chung, W.J.; Tanabe, T.; Telang, N.; Dannenberg, A.J. Resveratrol inhibits cyclooxygenase-2 transcription in human mammary epithelial cells. Ann. N. Y. Acad. Sci., 1999, 889(1), 214-223.
[http://dx.doi.org/10.1111/j.1749-6632.1999.tb08737.x] [PMID: 9705326]
[13]
Samy, R.P.; Thwin, M.M.; Gopalakrishnakone, P.; Ignacimuthu, S. Ethnobotanical survey of folk plants for the treatment of snakebites in Southern part of Tamilnadu, India. J. Ethnopharmacol., 2008, 115(2), 302-312.
[http://dx.doi.org/10.1016/j.jep.2007.10.006] [PMID: 18055146]
[14]
Al-Bayaty, F.H.; Abdulla, M.A.; Hassan, M.I.A.; Ali, H.M. Effect of Andrographis paniculata leaf extract on wound healing in rats. J.Nat. Prod.t Res.,, 2012,, 26(5), 423-429.
[http://dx.doi.org/10.1080/14786419.2010.496114] [PMID: 21660840]
[15]
Wasman, S.; Mahmood, A.; Chua, L.S.; Alshawsh, M.A.; Hamdan, S. Antioxidant and gastroprotective activities of Andrographis paniculata (Hempedu Bumi) in Sprague Dawley rats, 2011.https://www.ncbi.nlm.nih.gov/pubmed/22013743
[PMID: 22013743]
[16]
Jada, S.R.; Hamzah, A.S.; Lajis, N.H.; Saad, M.S.; Stevens, M.F.; Stanslas, J. Semisynthesis and cytotoxic activities of andrographolide analogues. J. Enzyme Inhib. Med. Chem., 2006, 21(2), 145-155.
[http://dx.doi.org/10.1080/14756360500499988] [PMID: 16789428]
[17]
Cheung, H-Y.; Cheung, S-H.; Li, J.; Cheung, C-S.; Lai, W-P.; Fong, W-F.; Leung, F-M. Andrographolide isolated from Andrographis paniculata induces cell cycle arrest and mitochondrial-mediated apoptosis in human leukemic HL-60 cells. Planta Med., 2005, 71(12), 1106-1111.
[http://dx.doi.org/10.1055/s-2005-873128] [PMID: 16395645]
[18]
Zhou, J.; Zhang, S.; Choon-, Nam, O; Shen, H-M. Critical role of pro-apoptotic Bcl-2 family members in andrographolide-induced apoptosis in human cancer cells. Biochem. Pharmacol., 2006, 72(2), 132-144.
[http://dx.doi.org/10.1016/j.bcp.2006.04.019] [PMID: 16740251]
[19]
Fitzgerald, J.B.; Schoeberl, B.; Nielsen, U.B.; Sorger, P.K. Systems biology and combination therapy in the quest for clinical efficacy. Nat. Chem. Biol., 2006, 2(9), 458..
[http://dx.doi.org/10.1038/nchembio817] [PMID: 16921358]
[20]
Yeh, P.; Kishony, R. Networks from drug-drug surfaces. Mol. Syst. Biol., 2007, 3(1), 85.
[http://dx.doi.org/10.1038/nchembio817] [PMID: 16921358]
[21]
Cheng, X.; Xiao, Y.; Wang, X.; Wang, P.; Li, H.; Yan, H.; Liu, Q. Anti-tumor and pro-apoptotic activity of ethanolic extract and its various fractions from Polytrichum commune L.ex Hedw in L1210 cells. J. Ethnopharmacol., 2012, 143(1), 49-56.
[http://dx.doi.org/10.1016/j.jep.2012.05.054] [PMID: 22687253]
[22]
Liu, B.; Ordonez-Ercan, D.; Fan, Z.; Edgerton, S.M.; Yang, X.; Thor, A.D. Downregulation of erbB3 abrogates erbB2-mediated tamoxifen resistance in breast cancer cells. Int. J. Cancer, 2007, 120(9), 1874-1882.
[http://dx.doi.org/10.1002/ijc.22423] [PMID: 17266042]
[23]
Chou, T-C. Drug combination studies and their synergy quantification using the Chou-Talalay method. Cancer Res., 2010, 70(2), 440-446.
[http://dx.doi.org/10.1158/0008-5472.CAN-09-1947] [PMID: 20068163]
[24]
Choi, M.S.; Kim, S.H.; Kuh, H.J. Penetration of paclitaxel and 5-fluorouracil in multicellular layers of human colorectal cancer cells. Oncol. Rep., 2011, 25(3), 863-870.
[http://dx.doi.org/10.3892/or.2011.1138] [PMID: 21225235]
[25]
Khan, I.; Yusuf, M.A.; Ansari, I.A.; Akhtar, M.S. In: Anticancer Plants: Mechanisms and Molecular Interactions;Springer,; , 2018,, pp. 143-163.https://www.springer.com/gp/book/9789811084164
[26]
Satyanarayana, C.; Deevi, D.S.; Rajagopalan, R.; Srinivas, N.; Rajagopal, S. DRF 3188 a novel semi-synthetic analog of andrographolide: cellular response to MCF 7 breast cancer cells. J.BMC Cancer, 2004, 4(1), 26.
[http://dx.doi.org/10.1186/1471-2407-4-26]
[27]
Khan, I.; Khan, F.; Farooqui, A.; Ansari, I.A. Andrographolide exhibits anticancer potential against human colon cancer cells by inducing cell cycle arrest and programmed cell death via augmentation of intracellular reactive oxygen species level. J. Nutr. Cancer, 2018, 70(5), 787-803.https://www.ncbi.nlm.nih.gov/pubmed/29781715
[PMID: 29781715]
[28]
Wong, H.C.; Sagineedu, S.R.; Lajis, N.H.; Loke, S.C.; Stanslas, J. Andrographolide induces cell cycle arrest and apoptosis in PC-3 prostate cancer cells. Afr. J. Pharm. Pharmacol., 2011, 5(2), 225-233.
[http://dx.doi.org/10.5897/AJMR10.383]
[29]
Yang, S.; Evens, A.M.; Prachand, S.; Singh, A.T.; Bhalla, S.; David, K.; Gordon, L.I. Mitochondrial-mediated apoptosis in lymphoma cells by the diterpenoid lactone andrographolide, the active component of Andrographis paniculata. J. Clin. Cancer Res., 2010, 16(19), 4755-4768.
[http://dx.doi.org/10.1158/1078-0432.CCR-10-0883] [PMID: 20798229]
[30]
Yang, S-H.; Wang, S-M.; Syu, J-P.; Chen, Y.; Wang, S-D.; Peng, Y-S.; Kuo, M-F.; Kung, H-N. Andrographolide induces apoptosis of C6 glioma cells via the ERK-p53-caspase 7-PARP pathway. BioMed Res. Int., 2014, 2014
[http://dx.doi.org/10.1155/2014/312847] [PMID: 25162007]
[31]
Dey, Y.N.; Kumari, S.; Ota, S.; Srikanth, N. Phytopharmacological review of Andrographis paniculata (Burm. f) Wall. ex Nees. Int. J.Nutr. Pharmacol. Neurol. Dis.,, 2013,, 3(1), 3..http://www.ijnpnd.com/text.asp?2013/3/1/3/106973
[32]
Hancke, J.; Burgos, R.; Caceres, D.; Wikman, G. A double‐blind study with a new monodrug Kan Jang: Decrease of symptoms and improvement in the recovery from common colds. Phytother. Res., 1995, 9(8), 559-562.
[http://dx.doi.org/10.1002/ptr.2650090804]
[33]
Chandrasekaran, C.V.; Thiyagarajan, P.; Sundarajan, K.; Goudar, K.S.; Deepak, M.; Murali, B.; Allan, J.J.; Agarwal, A. Evaluation of the genotoxic potential and acute oral toxicity of standardized extract of Andrographis paniculata (KalmCold). Food Chem. Toxicol., 2009, 47(8), 1892-1902.
[http://dx.doi.org/10.1016/j.fct.2009.05.006] [PMID: 19447157]
[34]
Horwitz, S.B. Taxol (paclitaxel): mechanisms of action. Ann. Oncol., 1994, 5(Suppl. 6), S3-S6.https://einstein.pure.elsevier.com/en/publications/taxol-paclitaxel-mechanisms-of-action-2
[35]
Wigmore, P.M. Mustafa, S.; El-Beltagy, M.; Lyons, L.; Umka, J.;Bennett, G. In: Chemo Fog;; Springer,, 2010,; pp. 157-164..
[http://dx.doi.org/10.1007/978-1-4419-6306-2_20] [PMID: 20738018]
[36]
Abdollahpour, N.; Asoodeh, A.; Saberi, M.R.; Chamani, J. Separate and simultaneous binding effects of aspirin and amlodipine to human serum albumin based on fluorescence spectroscopic and molecular modeling characterizations: A mechanistic insight for determining usage drugs doses. J. Lumin., 2011, 131(9), 1885-1899.
[http://dx.doi.org/10.1016/j.jlumin.2011.04.043]
[37]
Chamani, J. Energetic domains analysis of bovine α-lactalbumin upon interaction with copper and dodecyl trimethylammonium bromide. J. Mol. Struct., 2010, 979(1-3), 227-234.
[http://dx.doi.org/10.1016/j.molstruc.2010.06.035]
[38]
Cheung, M.T.; Ramalingam, R.; Lau, K.K.; Chiang, M.W.; Chiu, S.; Cheung, H.Y.; Lam, Y.W. Cell type-dependent effects of andrographolide on human cancer cell lines. Life Sci., 2012, 91(15-16), 751-760.
[http://dx.doi.org/10.1016/j.lfs.2012.04.009] [PMID: 23047020]
[39]
Yang, L.; Wu, D.; Luo, K.; Wu, S.; Wu, P. Andrographolide enhances 5-fluorouracil-induced apoptosis via caspase-8-dependent mitochondrial pathway involving p53 participation in hepatocellular carcinoma (SMMC-7721) cells. Cancer Lett., 2009, 276(2), 180-188.
[http://dx.doi.org/10.1016/j.canlet.2008.11.015] [PMID: 19097688]
[40]
Zhou, J.; Ong, C-N.; Hur, G-M.; Shen, H-M. Inhibition of the JAK-STAT3 pathway by andrographolide enhances chemosensitivity of cancer cells to doxorubicin. Biochem. Pharmacol., 2010, 79(9), 1242-1250.
[http://dx.doi.org/10.1016/j.bcp.2009.12.014] [PMID: 20026083]
[41]
Green, D.R.; Kroemer, G. The pathophysiology of mitochondrial cell death. J. Sci, 2004, 305(5684), 626-629.
[http://dx.doi.org/10.1126/science.1099320] [PMID: 15286356]
[42]
Green, D.R.; Reed, J.C. Mitochondria and apoptosis. J. Sci., 1998, 281(5381), 1309-1312.
[http://dx.doi.org/10.1146/annurev-genet-102108-134850] [PMID: 4762029]
[43]
Cohen, J.J. In: Advances in immunology; Elsevier, 1991, Vol. 50, pp. 55-85.https://www.elsevier.com/books/advances-in-immunology/dixon/978-0-12-022450-0
[44]
KIM, T.G.; HWI, K.K.; HUNG, C.S. Morphological and biochemical changes of andrographolide-induced cell death in human prostatic adenocarcinoma PC-3 cells. In Vivo, 2005, 19(3), 551-557.https://www.ncbi.nlm.nih.gov/pubmed/15875775
[PMID: 15875775]
[45]
Manikam, S.T.; Stanslas, J. Andrographolide inhibits growth of acute promyelocytic leukaemia cells by inducing retinoic acid receptor-independent cell differentiation and apoptosis. J. Pharm. Pharmacol., 2009, 61(1), 69-78.
[http://dx.doi.org/10.1211/jpp.61.01.0010] [PMID: 19126299]
[46]
Ott, M.; Gogvadze, V.; Orrenius, S.; Zhivotovsky, B. Mitochondria, oxidative stress and cell death. Apoptosis, 2007, 12(5), 913-922.
[http://dx.doi.org/10.1007/s10495-007-0756-2] [PMID: 17453160]
[47]
Valko, M.; Leibfritz, D.; Moncol, J.; Cronin, M.T.; Mazur, M.; Telser, J. Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol., 2007, 39(1), 44-84.
[http://dx.doi.org/10.1016/j.biocel.2006.07.001] [PMID: 16978905]
[48]
Bortner, C.D.; Oldenburg, N.B.; Cidlowski, J.A. The role of DNA fragmentation in apoptosis. Trends Cell Biol., 1995, 5(1), 21-26.
[http://dx.doi.org/10.1016/S0962-8924(00)88932-1] [PMID: 14731429]
[49]
Yang, L.; Wu, D.; Luo, K.; Wu, S.; Wu, P. Andrographolide enhances 5-fluorouracil-induced apoptosis via caspase-8-dependent mitochondrial pathway involving p53 participation in hepatocellular carcinoma (SMMC-7721) cells. Cancer Lett., 2009, 276(2), 180-188.
[http://dx.doi.org/10.1016/j.canlet.2008.11.015] [PMID: 19097688]

Rights & Permissions Print Cite
Article Metrics
36
1
© 2024 Bentham Science Publishers | Privacy Policy