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The Natural Products Journal

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ISSN (Print): 2210-3155
ISSN (Online): 2210-3163

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Research Article

Comparison of Hypoglycemic and Antioxidant Activities of the Compounds Isolated from Ficus hispida Linn. and Ficus pomifera Wall

Author(s): Ashalata Nepram, Sujata Wangkheirakpam and Warjeet S. Laitonjam*

Volume 10, Issue 5, 2020

Page: [621 - 630] Pages: 10

DOI: 10.2174/2210315509666191125101108

Price: $65

Abstract

Background: Traditional knowledge has been a legacy of the past to the present. Barks of Ficus hispida Linn. and leaves of Ficus pomifera Wall. (Moraceae) have been used traditionally for the treatment of diabetes in North-east India and many other places. As many drugs have been developed from traditional plants, the authors have taken up the plants for the study of hypoglycemic activity.

Objective: To investigate the hypoglycemic activities of the triterpenoids isolated from the plants and their antioxidant activities.

Methods: The bioactive compounds were determined by biochemical analysis, antioxidant activity using DPPH method. Hypoglycemic activity was detected using glucose tolerance test in normal rats and alloxan induced diabetic rats with Gliclazide as standard.

Results: The biochemicals and trace elements were present in appreciable amounts. Triterpenoids, (1-5), from F. pomifera and 19-hydroxyphlogacantholide (6), 3-O-[ß-D-glucopyranosyl-(1’→2’)-α- L-rhamnopyranosyl-phlogacanthoside] (7) and galanolactone (8) along with stigmasterol (9), stigmasta- 5,22-dien-7-on-3ß-ol (10), 5-(decahydro-1,1,4a-trimethyl-6-methylene-5-yl)-3-methylpent-2- enal (11), stigmasterol glucoside (12) and stigmast-4-en-3-one (13) from F. hispida Linn., respectively, were isolated. The different extracts of the barks and leaves of these plants along with the isolated compounds had antioxidant and hyploglycemic activities.

Conclusion: The five triterpenoids (1-5) were isolated from the methanol extract of the leaves of F. pomifera, and compounds (6-13) were isolated from the chloroform extract of the barks of F. hispida. Methanol extract of the leaves of F. pomifera and the chloroform extract of the barks of F. hispida; compounds (1-13) isolated from these two plants reduced DPPH free radicals in a concentrationdependent manner. It was also observed that the methanol and chloroform extracts of the plants, F. pomifera and F. hispida respectively, and the compounds (1, 6 & 7) exhibited anti-diabetic properties and also caused a highly significant reduction in the blood glucose levels of normal rats.

Keywords: Medicinal plants, antidiabetic, bioactive, ficus species, hypoglycemic, antioxidant.

Graphical Abstract

[1]
Ernst, E. Plants with hypoglycemic activity in humans. Phytomedicine, 1997, 4(1), 73-78.
[http://dx.doi.org/10.1016/S0944-7113(97)80031-1 ] [PMID: 23195249]
[2]
Perez, G, R.M.; Zavala, S, M.A.; Perez, G, S.; Perez, G, C. Antidiabetic effect of compounds isolated from plants. Phytomedicine, 1998, 5(1), 55-75.
[http://dx.doi.org/10.1016/S0944-7113(98)80060-3 ] [PMID: 23195700]
[3]
Sinha, S.C. Medicinal plants of Manipur, 3rd ed; Manipur Association for Sc. Soc.: Manipur, India, 1996.
[4]
Ghosh, R.; Saratchandra, Kh.; Rita, S.; Thockchom, I.S. Hypoglycemic activity of Ficus hispida (bark) in normal and diabetic albino rats. Indian J. Pharm., 2004, 36, 222-225.
[5]
Paarakh, P.M. Ficus racemosa Linn.-An overview. Nat. Prod. Radiance, 2009, 8, 84-90.
[6]
Ebungna, P.A.C.; Joshua, P.E.; Chigbo, M.U. Antihepatotoxic effects of Ficus vogelli ethanol leaf extracts on the liver function indices. J. Am. Sci., 2011, 7, 158-163.
[7]
Sapana, S.; Sujata, B.; Amruta, T.; Kalal, K.; Deshpande, N.R. GCMS study of hydrocarbons- a renewable biofuel with high calorific value from aerial roots of Ficus bengalensis Linn. EJEAF Chem., 2008, 7, 2743-2748.
[8]
Cherian, S.; Augusti, K.T. Antidiabetic effects of a glycoside of leucopelargonidin isolated from Ficus bengalensis Linn. Indian J. Exp. Biol., 1993, 31(1), 26-29.
[PMID: 8500813]
[9]
Bhar, P.; Thakur, S. Hydrocarbons from the leaves of Ficus hispida Roxb., Ficus bengalensis Linn. & Ficus infectoria Linn. Indian J. Chem., 1982, 20B, 722-723.
[10]
Wang, S.; Coviello, D.A. The isolation, characterization and synthesis of 10-ketotetracosyl arachidate from Ficus hispida. Tetrahedron, 1975, 31, 929-932.
[http://dx.doi.org/10.1016/0040-4020(75)80102-5]
[11]
Wangkheirakpam, S.D.; Wadawale, A.; Leishangthem, S.S.; Gurumayum, J.S.; Laitonjam, W.S. Cytotoxic triterpenoids from Ficus pomifera Linn. Indian J. Chem., 2015, 54B, 676-681.
[12]
Sun, H.X.; Zhang, J.X.; Ye, Y.P.; Pan, Y.J.; Shen, Y.A. Cytotoxic pentacyclic triterpenoids from the rhizomes of Astilbe chinensis. Helv. Chim. Acta, 2003, 86, 2414-2422.
[http://dx.doi.org/10.1002/hlca.200390194]
[13]
Foley, D.A.; O’Callaghan, Y.; O’Brien, N.M.; McCarthy, F.O.; Maguire, A.R. Synthesis and characterization of stigmasterol oxidation products. J. Agric. Food Chem., 2010, 58(2), 1165-1173.
[http://dx.doi.org/10.1021/jf9024745 ] [PMID: 20025271]
[14]
Hanson, J.R. Diterpenoids. Nat. Prod. Rep., 2006, 23(6), 875-885.
[http://dx.doi.org/10.1039/b516326a ] [PMID: 17119637]
[15]
Elgendya, E.M.; Ghamdyb, H.A. Thermal and photooxidation reactions of the steroids: Sitosterol, Stigmasterol and diosgenin. Taiwan Pharm. J., 2007, 59, 113-132.
[16]
Kovganko, N.V.; Kashkam, Z.N.; Borisov, E.V. 13c nmr spectra of sterol derivatives, intermediates in the synthesis of Ecdy- and brassinosteroids. Chem. Nat. Compd., 1999, 35, 642-645.
[http://dx.doi.org/10.1007/BF02236292]
[17]
Albert, W.W.; Davies-Coleman, M.T. Semisynthesis of lab-dane diterpene metabolites from the nudibranch Pleuro-branchaea meckelii. Tetrahedron, 2007, 63, 12179-12184.
[http://dx.doi.org/10.1016/j.tet.2007.09.039]
[18]
Greca, M.D.; Monaco, P.; Previtera, L. Stigmasterols from Typha latifolia. J. Nat. Prod., 1990, 53, 1430-1435.
[http://dx.doi.org/10.1021/np50072a005]
[19]
Blois, M.S. Antioxidant determinations by the use of a stable free radical. Nature, 1958, 181, 1199-1200.
[http://dx.doi.org/10.1038/1811199a0]
[20]
Laitonjam, W.S.; Kongbrailatpam, B.D. Studies on the chemical constituents and antioxidant activities of extracts from the roots of Smilax lanceaefolia Roxb. Nat. Prod. Res., 2010, 24(12), 1168-1176.
[http://dx.doi.org/10.1080/14786410903040402 ] [PMID: 20234971]
[21]
Laitonjam, W.S. Natural antioxidants (NAO) of plants acting as scavengers of free radicals. Studies in Natural Products Chemistry, Rahman, Atta-ur-, Ed.; Elsevier Science B. V: Amsterdam, Netherlands, 2012, 37, 259-275.
[22]
Lamela, M.; Cadavid, I.; Gato, A.; Calleja, J.M. Effects of Lythrum salicaria in normoglycemic rats. J. Ethnopharmacol., 1985, 14(1), 83-91.
[http://dx.doi.org/10.1016/0378-8741(85)90032-7 ] [PMID: 3910965]
[23]
Ford, E.S.; Will, J.C.; Bowman, B.A.; Narayan, K.M. Diabetes mellitus and serum carotenoids, finding from the third national and nutritional examination survey. Am. J. Epidemiol., 1999, 149(2), 168-176.
[http://dx.doi.org/10.1093/oxfordjournals.aje.a009783 ] [PMID: 9921962]

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