Interactions of Flavone and Steroid from A. subintegra as Potential Inhibitors for Porcine Pancreatic Lipase

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Author(s): Mastura Ibrahim, Saripah Salbiah Syed Abdul Azziz*, Chee Fah Wong, Yuhanis Mhd Bakri, Fauziah Abdullah.

Journal Name: Current Computer-Aided Drug Design

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Abstract:

Background: Obesity is one of serious health condition in the world which contributes too many of chronic diseases. The inhibition of pancreatic lipase is one of promising treatment of obesity problem.

Objective: The present study was designed to investigate anti-porcine pancreatic lipase effect of isolated compounds from Aquilaria subintegra and its mechanism.

Method: The isolation of crude extract was used serial column chromatography and identified using spectroscopic methods. Then the isolated compounds were assayed for anti-lipase properties using colorimetric assay. The prediction of energy binding between isolated compounds and enzyme was described using YASARA software.

Results: Four compounds were successfully isolated from bark of A. subintegra, namely, 5-hydroxy-7,4’-dimethoxyflavone, luteolin-7,3’,4’-trimethyl ether, 5,3’-dihydroxy-7,4’-dimethoxyflavone and β-sitosterol. The results indicated that all compounds were promise to inhibit pancreatic lipase activity in range between of 6% to 53%. This study showed that 5-hydroxy-7,4’-dimethoxyflavone was a competitive inhibitor and decreases the enzyme catalysis. Meanwhile β-sitosterol was a non- competitive inhibitor since the latter was bind allosterically toward enzyme.

Conclusion: This finding is significant for further investigation of bioactive compounds from A. subintegra on animal study.

Keywords: Aquilaria subintegra, lipase, flavonoid, docking, competitive, YASARA

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(E-pub Ahead of Print)
DOI: 10.2174/1573409915666191015112320
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