Genome editing refers to changing the genome sequence of an organism by knockout, insertion, and
site mutation, resulting in changes in the genetic information of the organism. The clustered regularly interspaced
short palindromic repeats (CRISPR)/ CRISPR-associated protein-9 nuclease (Cas9) system is a genome
editing technique developed by the acquired immune system in the microbes, such as bacteria and archaebacteria,
which targets and edits genome sequences according to the principle of complementary base pairing. This
technique can be used to edit endogenous genomic DNA sequences in organisms accurately and has been widely
used in fields, such as biotechnology, cancer gene therapy, and dermatology. In this review, we summarize the
history, structure, mechanism, and application of CRISPR/Cas9 in gene therapy and dermatological diseases.