Enzymatic Textile Dyes Decolorization by In vitro and In silico Studies

Author(s): Sridevi Ayla*, Monika Kallubai, Suvarnalatha Devi Pallipati, Golla Narasimha.

Journal Name: Recent Patents on Biotechnology

Volume 13 , Issue 4 , 2019

Become EABM
Become Reviewer

Graphical Abstract:


Abstract:

Background: Laccase, a multicopper oxidoreductase (EC: 1.10.3.2), is a widely used enzyme in bioremediation of textile dye effluents. Fungal Laccase is preferably used as a remediating agent in the treatment and transformation of toxic organic pollutants. In this study, crude laccase from a basidiomycetes fungus, Phanerochaete sordida, was able to decolorize azo, antroquinone and indigoid dyes. In addition, interactions between dyes and enzyme were analysed using molecular docking studies.

Methods: In this work, a white rot basidiomycete’s fungus, Phanerochaete sordida, was selected from forest soil isolates of Eastern Ghats, and Tirumala and lignolytic enzymes production was assayed after 7 days of incubation. The crude enzyme was checked for decolourisation of various synthetic textile dyes (Vat Brown, Acid Blue, Indigo, Reactive Blue and Reactive Black). Molecular docking studies were done using Autodock-4.2 to understand the interactions between dyes and enzymes.

Results: Highest decolourisation efficiency was achieved with the crude enzyme in case of vat brown whereas the lowest decolourisation efficiency was achieved in Reactive blue decolourisation. Similar results were observed in their binding affinity with lignin peroxidase of Phanerochaete chrysosporium through molecular docking approach.

Conclusion: Thus, experimental results and subsequent in silico validation involving an advanced remediation approach would be useful to reduce time and cost in other similar experiments.

Keywords: Laccase, textile dyes, bioremediation, Phanerochaete sordida, molecular docking, decolorization.

[1]
Velu C, Veeramani E, Suntharam S, Kalimuthu K. In silico screening and comparative study on the effectiveness of textile dye decolourization by crude laccase immobilised alginate encapsulated beads from Pleurotus Ostreatus. J Bioprocess Biotech 2012; 2011
[2]
Revankar MS, Lele SS. Synthetic dye decolorization by white rot fungus, Ganoderma sp. WR-1. Bioresour Technol 2007; 98(4): 775-80.
[3]
Forgacs E, Cserháti T, Oros G. Removal of synthetic dyes from wastewaters: a review. Environ Int 2004; 30(7): 953-71.
[4]
Robinson T, McMullan G, Marchant R, Nigam P. Remediation of dyes in textile effluent: a critical review on current treatment technologies with a proposed alternative. Bioresour Technol 2001; 77(3): 247-55.
[5]
Thurston CF. The structure and function of fungal laccases. Microbiology 1994; 140: 19-26.
[6]
Sette LD, de Oliveira VM, Rodrigues MFA. Microbial lignocellulolytic enzymes: industrial applications and future perspectives. Microbiol Aust 2008; 29: 18-20.
[7]
Padmanaban V, Prakash S, Sherildas P, Jacob J, Nelliparambil K. Biodegradation of anthraquinone based compounds. review. Int J Adv Res Eng Technol 2013; 4: 74-83.
[8]
Toh Y-C, Yen JJL, Obbard JP, Ting Y-P. Decolourisation of azo dyes by white-rot fungi (WRF) isolated in Singapore. Enzyme Microb Technol 2003; 33: 569-75. [http://dx.doi.org/10.1016/S0141-0229(03)00177-7].
[9]
McMullan G, Meehan C, Conneely A, et al. Microbial decolourisation and degradation of textile dyes. Appl Microbiol Biotechnol 2001; 56(1-2): 81-7.
[10]
Singhal V, Rathore V. Effects of Zn2+ and Cu2+ on growth, lignin degradation and ligninolytic enzymes in Phanerochaete chrysosporium. World J Microbiol Biotechnol 2001; 17: 235-40.
[11]
Ramsay JA, Goode C. Decoloration of a carpet dye effluent using Trametes versicolor. Biotechnol Lett 2004; 26(3): 197-201.
[12]
Kaushik P, Malik A. Fungal dye decolourization: recent advances and future potential. Environ Int 2009; 35(1): 127-41.
[13]
Hadibarata T, Yusoff ARM, Aris A, Hidayat T, Kristanti RA. Decolorization of azo, triphenylmethane and anthraquinone dyes by laccase of a newly isolated Armillaria sp. F022. Water Air Soil Pollut 2012; 223: 1045-54.
[14]
Hadibarata T, Yusoff ARM, Kristanti RA. Decolorization and metabolism of anthraquionone-type dye by laccase of white-rot fungi Polyporus sp. S133. Water Air Soil Pollut 2012; 223: 933-41.
[15]
Chandralata RK, Donna TDT. A novel process for the decolourisation of colored effluents. WO2006059348A1 2007.
[16]
Hong Y, Xiao Y, Zhou H, et al. Expression of a laccase cDNA from Trametes sp. AH28-2 in Pichia pastoris and mutagenesis of transformants by nitrogen ion implantation. FEMS Microbiol Lett 2006; 258(1): 96-101.
[17]
Eichlerova I, Homolka L, Nerud F. Decolorization of high concentrations of synthetic dyes by the white rot fungus Bjerkandera adusta strain CCBAS232. Dyes Pigments 2007; 75: 38-44.
[18]
Das N, Sengupta S, Mukherjee M. Importance of laccase in vegetative growth of pleurotus Florida. Appl Environ Microbiol 1997; 63(10): 4120-2.
[19]
Wood W, Kellogg S. Methods in enzymology - Biomass, part b, Lignin, Pectin and Chitin. 1st ed. San Diego, CA: Academic Press 1988.
[20]
Bonnen AM, Anton LH, Orth AB. Lignin-degrading enzymes of the commercial button mushroom, Agaricus bisporus. Appl Environ Microbiol 1994; 60(3): 960-5.
[21]
Morris GM, Goodsell DS, Huey R, Olson AJ. Distributed automated docking of flexible ligands to proteins: parallel applications of AutoDock 2.4. J Comput Aided Mol Des 1996; 10(4): 293-304.
[22]
Morris GM, Huey R, Lindstrom W, et al. AutoDock4 and AutoDockTools4: automated docking with selective receptor flexibility. J Comput Chem 2009; 30(16): 2785-91.
[23]
Wallace AC, Laskowski RA, Thornton JM. LIGPLOT: a program to generate schematic diagrams of protein-ligand interactions. Protein Eng 1995; 8(2): 127-34.
[24]
Delano WL. The PyMOL molecular graphics system 2002.
[25]
Kjøller AH, Struwe S. Fungal communities, succession, enzymes, and decomposition Enzymes in the environment: activity, ecology and applications. New York: Marcel Dekker 2002; pp. 267-84.
[26]
Baldrian P. Enzymes of saprotrophic basidiomycetes. Br Mycol Soc Symp 2008; 28: 19-41.
[27]
Baldrian P. Ectomycorrhizal fungi and their enzymes in soils: is there enough evidence for their role as facultative soil saprotrophs? Oecologia 2009; 161(4): 657-60.
[28]
Baldrian P, Voříšková J, Dobiášová P, Merhautová V, Lisá L, Valášková V. Production of extracellular enzymes and degradation of biopolymers by saprotrophic microfungi from the upper layers of forest soil. Plant Soil 2011; 338: 111-25.
[29]
Šnajdr J, Valášková V, Merhautová V, Cajthaml T, Baldrian P. Activity and spatial distribution of lignocellulose-degrading enzymes during forest soil colonization by saprotrophic basidiomycetes. Enzyme Microb Technol 2008; 43: 186-92.
[30]
Steffen KT, Schubert S, Tuomela M, Hatakka A, Hofrichter M. Enhancement of bioconversion of high-molecular mass polycyclic aromatic hydrocarbons in contaminated non-sterile soil by litter-decomposing fungi. Biodegradation 2007; 18(3): 359-69.
[31]
Valášková V, Šnajdr J, Bittner B, et al. Production of lignocellulose-degrading enzymes and degradation of leaf litter by saprotrophic basidiomycetes isolated from a Quercus petraea forest. Soil Biol Biochem 2007; 39: 2651-60.
[32]
Park H-M, Kim T-W, Hwang S-J, Choy J-H. Chemical bonding nature and mesoporous structure of nickel intercalated montmorillonite clay. Bull Korean Chem Soc 2006; 27: 1323-8.
[33]
Hsu CA, Wen TN, Su YC, Jiang ZB, Chen CW, Shyur LF. Biological degradation of anthroquinone and azo dyes by a novel laccase from Lentinus sp. Environ Sci Technol 2012; 46(9): 5109-17.
[34]
Decolorization of reactive dye by white-rot fungus Datronia sp. KAPI0039. Witthayasan Kasetsat Witthayasat 2010; 44: 879-90.
[35]
Senthilkumar S, Perumalsamy M, Prabhu HJ. Decolourization potential of white-rot fungus Phanerochaete chrysosporium on synthetic dye bath effluent containing Amido black 10B. J Saudi Chem Soc 2014; 18: 845-53.
[36]
Chandralata RK, Trevor MD, Thorn RG, Reddy CA. White rot lignin modifying fungus Flavodon flavus and a process for removing dye from dye containing water or soils. US6395534B1 2002.
[37]
Shantikriti S, Gnanendra S, Swati VS, Jadav JP, Sadasivam SK. In silico analysis of bacterial systems for textile azo dye decolorization and affirmation with wetlab studies.Soil Air Water. 2017; 45: pp. (9)1-16.


Rights & PermissionsPrintExport Cite as

Article Details

VOLUME: 13
ISSUE: 4
Year: 2019
Page: [268 - 276]
Pages: 9
DOI: 10.2174/1872208313666190625123847
Price: $58

Article Metrics

PDF: 19
HTML: 2