Design Based Ultrasound-Assisted Extraction of Marrubium Vulgare Linn and Comparative Evaluation of Extracts for Furan Labdane Diterpene (Marrubiin) Concentration and Antihypertensive Potential

(E-pub Ahead of Print)

Author(s): Arun Nanda, Vineet Mittal*.

Journal Name: Current Bioactive Compounds

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Background: Improvement in extract quality in terms of concentration of secondary metabolites and pharmacological activity has always been the need of hour. In the present research, the target was to extract the selected medicinal herb using the ultrasound waves and to optimize the extraction conditions for the improvement in the quality of extract with respect to furan labdane diterpene (marrubiin) concentration and antihypertensive potential.

Methods: The whole plant of Marrubium vulgare Linn. was collected from the fields of Pulwama district of Jammu and Kashmir state in India and extracted by cold maceration (MVM) and ultrasound assisted extraction techniques (MVU). The response surface methodology coupled with central composite design was employed to optimize the selected extraction parameters in UAE method. The marrubiin concentration in different extracts was determined by HPTLC. The extracts were also evaluated for the antihypertensive potential by non invasive blood pressure monitoring (NIBPM) method.

Results: The extract yield (14.2 ± 0.9%) and concentration of marrubiin (0.91 ± 0.04%) was significantly improved at the optimized UAE conditions (Ultrasound power 467 W, sonication time of 47 minutes and solvent concentration of 33 mL per g of drug) as compared to conventional method. Also the MVU extract (200 mg/kg) along with ethanol significantly (p<0.01) prevented the rise in mean systolic blood pressure (MSBP) of animals and also the GSH was significantly (p<0.05) enhanced as compared to ethanol treated animals.

Conclusion: The elevation in MSBP and decrease in reduced glutathione concentration (GSH) by the chronic ethanol consumption was significantly altered by MVU extract as compared to MVM extract. The enhanced antihypertensive effect of selected herb may be attributed to the improved concentration of secondary metabolites (marrubiin) in MVU extract obtained at optimized conditions.

Keywords: Central composite design, Glutathione, High performance thin layer chromatography, Mean systolic blood pressure, Scanning electron micrography

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(E-pub Ahead of Print)
DOI: 10.2174/1573407215666190524102431
Price: $95