Login Register Cart 0
Generic placeholder image

Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry

Editor-in-Chief

ISSN (Print): 1871-5230
ISSN (Online): 1875-614X

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Phycocyanin Extracted from Oscillatoria minima Shows Antimicrobial, Algicidal, and Antiradical Activities: In silico and In vitro Analysis

Author(s): Vaishali C. Venugopal*, Abhimanyu Thakur, Latha K. Chennabasappa, Gaurav Mishra, Kunal Singh, Parth Rathee and Anjali Ranjan

Volume 19, Issue 3, 2020

Page: [240 - 253] Pages: 14

DOI: 10.2174/1871523018666190405114524

Abstract

Background: Phycocyanin is an algae-derived protein, which binds to pigment for harvesting light. It has been reported in various different species, including that of red algae, dinoflagellates, and cryptophyta. Importantly, phycocyanin has enormous applications, including cosmetic colorant, food additive, biotechnology, diagnostics, fluorescence detection probe, an anticancer agent, anti-inflammatory, immune enhancer, etc. In addition, several different algae were utilized for the isolation of cyano-phycocyanin (C-PC), but most of the purification methods consist of several steps of crude extraction.

Aim: To isolate C-PC from a new source of microalgae with better purity level and to evaluate its antimicrobial, algicidal, and antiradical activities.

Methods: Biological activity, permeability, pharmacokinetics, and toxicity profile of C-PC were predicted by in silico studies. C-PC was purified and isolated by using ammonium sulphate precipitation, ion-exchange chromatography and gel-filtration chromatography. C-PC was characterized by SDS-PAGE and elution profile (purity ratio) analysis. Antimicrobial and algicial activities of C-PC were evaluated by the microtitre plate based assays. Antiradical activity of C-PC was evaluated by DPPH- and ABTS*+ radical scavenging assays.

Conclusion: C-PC was extracted from Oscillatoria minima for the first time, followed by its quantitative as well qualitative evaluation, indicating a new alternative source of this important protein. Furthermore, the antimicrobial, algicidal, and antiradical activities of the isolated C-PC extract have been demonstrated by both in silico as well as in vitro methods.

Keywords: Algicidal, antimicrobial, antiradical, cyanophycocyanin, microalgae, Oscillatoria minima, pharmaceutical drugs.

« Previous Next »
Graphical Abstract

[1]
Liu, Q.; Huang, Y.; Zhang, R.; Cai, T.; Cai, Y. Medical application of Spirulina platensis derived C-phycocyanin. Evid. Based Complement. Alternat. Med., 2016, 2016, 7803846.
[PMID: 27293463]
[2]
Glazer, A.N. Phycobilisome a macromolecular complex optimized for light energy transfer. BBA- Bioenergetics, 1984, 768, 29-51.
[3]
Kumar, D.; Dhar, D.W.; Pabbi, S.; Kumar, N.; Walia, S. Extraction and purification of C-phycocyanin from Spirulina platensis (CCC540). Indian J. Plant. Physiol., 2014, 19, 184-188.
[http://dx.doi.org/10.1007/s40502-014-0094-7] [PMID: 25089058]
[4]
Silveira, S.T.; Quines, L.K.; Burkert, C.A.; Kalil, S.J. Separation of phycocyanin from Spirulina platensis using ion exchange chromatography. Bioprocess Biosyst. Eng., 2008, 31(5), 477-482.
[http://dx.doi.org/10.1007/s00449-007-0185-1] [PMID: 18219495]
[5]
Wang, L.; Qu, Y.; Fu, X.; Zhao, M.; Wang, S.; Sun, L. Isolation, purification and properties of an R-Phycocyanin from the phycobilisomes of a marine red macroalga Polysiphonia urceolata. PLoS One, 2014, 9(2), e87833.
[http://dx.doi.org/10.1371/journal.pone.0087833] [PMID: 24504114]
[6]
Niu, J.F.; Wang, G.C.; Lin, X.Z.; Zhou, B.C. Large-scale recovery of C-phycocyanin from Spirulina platensis using expanded bed adsorption chromatography. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2007, 850(1-2), 267-276.
[http://dx.doi.org/10.1016/j.jchromb.2006.11.043] [PMID: 17178463]
[7]
Chaiklahan, R.; Chirasuwan, N.; Loha, V.; Tia, S.; Bunnag, B. Separation and purification of phycocyanin from Spirulina sp. using a membrane process. Bioresour. Technol., 2011, 102(14), 7159-7164.
[http://dx.doi.org/10.1016/j.biortech.2011.04.067] [PMID: 21570281]
[8]
Oliveira, E.G.; Rosa, G.S.; Moraes, M.A.; Pinto, L.A. Characterization of thin layer drying of Spirulina platensis utilizing perpendicular air flow. Bioresour. Technol., 2009, 100(3), 1297-1303.
[http://dx.doi.org/10.1016/j.biortech.2008.05.052] [PMID: 18922691]
[9]
Bryant, D.A. Phycoerythrocyanin and phycoerythrin: properties and occurrence in cyanobacteria. Microbiology, 1982, 128, 835-844.
[http://dx.doi.org/10.1099/00221287-128-4-835]
[10]
Kooistra, W.H.; Gersonde, R.; Medlin, L.K.; Mann, D.G. The origin and evolution of the diatoms: their adaptation to a planktonic existence. In: Evolution of primary producers in the sea; , 2007; pp. 207-249.
[http://dx.doi.org/10.1016/B978-012370518-1/50012-6]
[11]
Yéprémian, C.; Catherine, A.; Bernard, C.; Congestri, R.; Elersek, T.; Pilkaityte, R. Phycocyanin extraction and determination.Handbook of Cyanobacterial Monitoring and Cy-anotoxin Analysis; , 2016, 20, pp. 335-338.
[12]
Gantar, M.; Dhandayuthapani, S.; Rathinavelu, A. Phycocyanin induces apoptosis and enhances the effect of topotecan on prostate cell line LNCaP. J. Med. Food, 2012, 15(12), 1091-1095.
[http://dx.doi.org/10.1089/jmf.2012.0123] [PMID: 23134462]
[13]
Thakur, A.; Roy, A.; Ghosh, A.; Chhabra, M.; Banerjee, S. Abiraterone acetate in the treatment of prostate cancer. Biomed. Pharmacother., 2018, 101, 211-218.
[http://dx.doi.org/10.1016/j.biopha.2018.02.067] [PMID: 29494958]
[14]
Bharathiraja, S.; Seo, H.; Manivasagan, P.; Santha Moorthy, M.; Park, S.; Oh, J. In-vitro photodynamic effect of phycocyanin against breast cancer cells. Molecules, 2016, 21(11), 1470.
[http://dx.doi.org/10.3390/molecules21111470] [PMID: 27827890]
[15]
Bhayani, K.; Mitra, M.; Ghosh, T.; Mishra, S. C-Phycocyanin as a potential biosensor for heavy metals like Hg2+ in aquatic systems. RSC Advances, 2016, 6, 111599-111605.
[http://dx.doi.org/10.1039/C6RA22753H]
[16]
Agrawal, M.; Yadav, S.K.; Agrawal, S.K.; Karmakar, S. Nutraceutical phycocyanin nanoformulation for efficient drug delivery of paclitaxel in human glioblastoma U87MG cell line. J. Nanopart. Res., 2017, 19, 272.
[http://dx.doi.org/10.1007/s11051-017-3972-x]
[17]
McCarty, M.F. Clinical potential of Spirulina as a source of phycocyanobilin. J. Med. Food, 2007, 10(4), 566-570.
[http://dx.doi.org/10.1089/jmf.2007.621] [PMID: 18158824]
[18]
Romay, Ch.; González, R.; Ledón, N.; Remirez, D.; Rimbau, V. C-phycocyanin: a biliprotein with antioxidant, anti-inflammatory and neuroprotective effects. Curr. Protein Pept. Sci., 2003, 4(3), 207-216.
[http://dx.doi.org/10.2174/1389203033487216] [PMID: 12769719]
[19]
Min, S.K.; Park, J.S.; Luo, L.; Kwon, Y.S.; Lee, H.C.; Shim, H.J.; Kim, I.D.; Lee, J.K.; Shin, H.S. Assessment of C-phycocyanin effect on astrocytes-mediated neuroprotection against oxidative brain injury using 2D and 3D astrocyte tissue model. Sci. Rep., 2015, 5, 14418.
[http://dx.doi.org/10.1038/srep14418] [PMID: 26399322]
[20]
El-Sheekh, M.M.; Daboor, S.M.; Swelim, M.A.; Mohamed, S. Production and characterization of antimicrobial active substance from Spirulina platensis. Iran. J. Microbiol., 2014, 6(2), 112-119.
[PMID: 25705362]
[21]
Shasank, S. Swain, Sudhir, K.; Paidesetty, R. Antibacterial, antifungal and antimycobacterial compounds from cyanobacteria. Biomed. Pharmacother., 2017, 90, 760-776.
[http://dx.doi.org/10.1016/j.biopha.2017.04.030]
[22]
Jiang, L.; Wang, Y.; Yin, Q.; Liu, G.; Liu, H.; Huang, Y.; Li, B. Phycocyanin: a potential drug for cancer treatment. J. Cancer, 2017, 8(17), 3416-3429.
[http://dx.doi.org/10.7150/jca.21058] [PMID: 29151925]
[23]
Patel, A.; Mishra, S.; Pawar, R.; Ghosh, P.K. Purification and characterization of C-Phycocyanin from cyanobacterial species of marine and freshwater habitat. Protein Expr. Purif., 2005, 40(2), 248-255.
[http://dx.doi.org/10.1016/j.pep.2004.10.028] [PMID: 15766866]
[24]
Minkova, K.M.; Tchernov, A.A.; Tchorbadjieva, M.I.; Fournadjieva, S.T.; Antova, R.E.; Busheva, M.Ch. Purification of C-phycocyanin from Spirulina (Arthrospira) fusiformis. J. Biotechnol., 2003, 102(1), 55-59.
[http://dx.doi.org/10.1016/S0168-1656(03)00004-X] [PMID: 12668314]
[25]
Lagunin, A.; Stepanchikova, A.; Filimonov, D.; Poroikov, V. PASS: prediction of activity spectra for biologically active substances. Bioinformatics, 2000, 16(8), 747-748.
[http://dx.doi.org/10.1093/bioinformatics/16.8.747] [PMID: 11099264]
[26]
Daina, A.; Michielin, O.; Zoete, V. SwissADME: a free web tool to evaluate pharmacokinetics, drug-likeness and medicinal chemistry friendliness of small molecules. Sci. Rep., 2017, 7, 42717.
[http://dx.doi.org/10.1038/srep42717] [PMID: 28256516]
[27]
Bennett, A.; Bogorad, L. Complementary chromatic adaptation in a filamentous blue-green alga. J. Cell Biol., 1973, 58(2), 419-435.
[http://dx.doi.org/10.1083/jcb.58.2.419] [PMID: 4199659]
[28]
Himmelhoch, S.R. Chromatography of proteins on ion-exchange adsorbents. Methods Enzymol., 1971, 22, 273-286.
[http://dx.doi.org/10.1016/0076-6879(71)22028-0]
[29]
Volk, R.B.; Furkert, F.H. Antialgal, antibacterial and antifungal activity of two metabolites produced and excreted by cyanobacteria during growth. Microbiol. Res., 2006, 161(2), 180-186.
[http://dx.doi.org/10.1016/j.micres.2005.08.005] [PMID: 16427523]
[30]
Devi, K.P.; Suganthy, N.; Kesika, P.; Pandian, S.K. Bioprotective properties of seaweeds: in vitro evaluation of antioxidant activity and antimicrobial activity against food borne bacteria in relation to polyphenolic content. BMC Complement. Altern. Med., 2008, 8, 38.
[http://dx.doi.org/10.1186/1472-6882-8-38] [PMID: 18613983]
[31]
Kedare, S.B.; Singh, R.P. Genesis and development of DPPH method of antioxidant assay. J. Food Sci. Technol., 2011, 48(4), 412-422.
[http://dx.doi.org/10.1007/s13197-011-0251-1] [PMID: 23572765]
[32]
Dong, J.W.; Cai, L.; Xing, Y.; Yu, J.; Ding, Z.T. Re-evaluation of ABTS*+ assay for total antioxidant capacity of natural products. Nat. Prod. Commun., 2015, 10(12), 2169-2172.
[http://dx.doi.org/10.1177/1934578X1501001239] [PMID: 26882692]
[33]
Manirafasha, E.; Ndikubwimana, T.; Zeng, X.; Lu, Y.; Jing, K. Phycobiliprotein: potential microalgae derived pharmaceutical and biological reagent. Biochem. Eng. J., 2016, 109, 282-296.
[http://dx.doi.org/10.1016/j.bej.2016.01.025]
[34]
Re, R.; Pellegrini, N.; Proteggente, A.; Pannala, A.; Yang, M.; Rice-Evans, C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med., 1999, 26(9-10), 1231-1237.
[http://dx.doi.org/10.1016/S0891-5849(98)00315-3] [PMID: 10381194]

Rights & Permissions Print Cite
Article Metrics
35
© 2024 Bentham Science Publishers | Privacy Policy