Background: Herpes simplex virus (HSV) and poliovirus (PV) are both agents of major
concern in the public health system. It has been shown that Dimorphandra gardneriana galactomannans
can be used as solubilizer vehicles in the manufacturing of medicine. Mangiferin is the major
constituent of Mangifera indica and presents multiple medicinal and biological activities.
Objective: This study assayed the effect of D. gardneriana galactomannan combined with mangiferin
(DgGmM) against HSV-1 and PV-1.
Methods: The DgGmM cytotoxicity was evaluated by the colorimetric MTT method and the antiviral
activity by plaque reduction assay, immunofluorescence and polymerase chain reaction (PCR), in
Results: The DgGmM showed a 50% cytotoxic concentration (CC50) > 2000 µg/mL. The 50% inhibitory
concentrations (IC50) for HSV-1 and PV-1 were, respectively, 287.5 µg/mL and 206.2 µg/mL,
with selectivity indexes (SI) > 6.95 for the former and > 9.69 for the latter. The DgGmM time-ofaddition
protocol for HSV-1 showed a maximum inhibition at 500 µg/mL, when added concomitantly
to infection and at the time 1 h post-infection (pi). While for PV-1, for the same protocol, the greatest
inhibition, was also observed concomitantly to infection at 500 μg/mL and at the times 4 h and 8 h pi.
The inhibition was also demonstrated by the decrease of fluorescent cells and/or the inhibition of specific
Conclusion: These results suggested that the DgGmM inhibited HSV-1 and PV-1 replication, with low
cytotoxicity and high selectivity and, therefore, represents a potential candidate for further studies on
the control of herpes and polio infections.