Background: Calendula arvensis is an annual Mediterranean plant growing in Morocco between
Rabat and Khemissat. C. arvensisis is known in folk medicine as an anti-inflammatory and antipyretic
remedy. However, few reports have investigated its pharmacological properties.
Methods: The objective of the present study was to determine chemical composition of C. arvensis
flowers, and to investigate their antidiabetic activities by mean of digestive enzyme inhibition. The profile
of phenolic compounds was established by HPLC-DAD-QTOF-MS analysis. While the antidiabetic
activity was evaluated by the in vitro enzyme inhibition assays.
Results: Phytochemical analysis revealed the presence of anthocyanins, flavonoids, tannins, and saponins
as major elements. Whereas, alkaloids and terpenes were not detected in the plant samples. The
chromatographic quantification identified 18 metabolites, with the caffeic acid as a major element. C.
arvensis aqueous and methanolic extracts exhibited higher inhibitory potential against α-amylase, α-
glucosidase and ß-galactosidase compared to the hexanic extract.
Conclusion: The present study brings evidence to the hypoglycemic effect of C. arvensis flowers
through enzyme inhibitory activities, and identifies the possible phenolic compounds associated with