Background: Cervical cancer arises from the cervix and it is the 3rd most diagnosed malignancy
and a foremost cause of cancer-related death in females. On the other hand, the expressions of
EGFR and p53 are two important proteins observed in various studies on cervical cancer.
Objective: The study aims to evaluate the beneficial effect of radiotherapy based on the regulation of
p53 and EGFR gene in patients with cervical cancer.
Method: In this investigation, the regulation of important molecules responsible for cancer cell proliferation
and DNA repair in the cervical cancer cell line was evaluated. The study comprises of an
evaluation based on clinical study design from the malignant biopsies of 15 cervical cancer patients.
The patterns of expression for the p53 gene and epidermal growth factor receptor (EGFR) were evaluated
in DoTc2 and SiHa cervical cancer cell lines using clonogenic assay, western blotting and immunohistochemistry
techniques from the malignant biopsies of the 15 patients.
Results: The study observed that the regulation of p53 and EGFR was very weak after the exposure of
the radiation. In addition, the expression of p53 and EGFR was observed in malevolent biopsy samples
after radiation with a dosage of 1.8 Gy radiations. Additionally, the expression of p53 and EGFR was
able to induce by a single dose of radiotherapy in the malignant biopsies whereas it was unable to induce
in DoTc2 and SiHa cervical cancer cells.
Conclusion: The study observed that radiation exposed cancer cell lines modulates the expression of p53
and EGFR gene. The study also highlights the gap between in vitro experimental models and clinical