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The Natural Products Journal

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ISSN (Print): 2210-3155
ISSN (Online): 2210-3163

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Research Article

Antimicrobial and Antioxidant Compounds in Endophyte Isolate L-003 Obtained from the Aquatic Plant Nelumbo nucifera

Author(s): Papiya Chakravorty, Nidhi Srivastava, Ahongshangbam Ibeyaima and Indira P. Sarethy*

Volume 10, Issue 2, 2020

Page: [139 - 144] Pages: 6

DOI: 10.2174/2210315509666190114143222

Price: $65

Abstract

Background: Microorganisms from understudied habitats have been shown to be an important source of novel bioactive compounds. Endophytes constitute an underexplored group of microorganisms, of which those from aquatic plants have been even less studied. Nelumbo nucifera (lotus) is an aquatic plant with medicinal properties. A screening program for endophytes from N. nucifera by our research group resulted in many microbial isolates, of which isolate L-003 was a promising candidate, exhibiting antimicrobial and antioxidant activities.

Objectives: The major objectives were to characterize the endophyte L-003 for its antimicrobial and antioxidant properties, identify the constituent bioactive compounds by GC-MS and characterize their activities further using in silico software.

Methods: L-003 was identified by PIBWin software. Antimicrobial activity of the aqueous and organic extracts of culture supernatant of L-003 was checked against a panel of bacteria and fungi. Since the ethyl acetate extract showed the best antimicrobial activity, it was further characterized by thin layer chromatography, an activity confirmed by bioautography and purified by column chromatography. Total antioxidant capacity was assayed by standard techniques. Partially purified metabolite fingerprints were identified by GC-MS analysis.

Results: Based on morphological and biochemical analyses, isolate L-003 was identified as belonging to Streptococcus sp. All the organic solvent extracts showed antimicrobial activity. Ethyl acetate extract showed maximum antimicrobial activity against all selected targets and exhibited antioxidant activity too, though butanol and aqueous extracts showed higher antioxidant activity. Two compounds, Acetic acid,-hydroxy, methyl ester and Disulfide, dipropyl, were identified by GC-MS in the metabolite fingerprint. These compounds showed differences in observed and calculated retention indices and could, therefore, be novel. In silico activity, characterization confirmed the antimicrobial and antioxidant properties attributed to these compounds.

Conclusion: This is the first study reporting metabolite fingerprinting, identification and characterization of bioactive compounds from an endophytic isolate of Nelumbo nucifera. We conclude that endophytes from aquatic plants could be prospective sources of bioactive compounds, in this case with antimicrobial and antioxidant activities.

Keywords: Nelumbo nucifera, endophyte, antimicrobial, antioxidant, GC-MS, PASS.

Graphical Abstract

[1]
Christina, A.; Varghese, C.; Bhore, S.J. Endophytic bacteria as a source of novel antibiotics: An overview. Pharm. Rev., 2013, 7(13), 11-16.
[http://dx.doi.org/10.4103/0973-7847.112833]
[2]
Santoyo, G.; Moreno-Hagelsieb, G.; Orozco-Mosqueda, M.; Del, C.; Glick, B. R. Plant growth-promoting bacterial endophytes. Microbiol. Res, 2016, 183, 92-99.
[http://dx.doi.org/10.1016/j .micres. 2015.11.008.]
[3]
Strobel, G.; Daisy, B. Bioprospecting for microbial endophytes and their Natural Products. Microbiol. Mol. Biol. Rev., 2003, 67(4), 491-502.
[4]
Ijarwal, T.; Sharma, B.; Khan, F.; Ibeyaima, A.; Dwivedi, A.; Saini, N.; Sarethy, I.P. Endophytes from the aquatic plant Nelumbo nucifera: Diversity profile and activity characterization. Int. J. Pharm. Pharm. Sci., 2016, 8(1), 266-270.
[5]
Bryant, T.N. PIBWin software for probabilistic identification. J. Appl. Microbiol., 2004, 97(6), 1326-1327.
[http://dx.doi.org/10.1111/j.1365-2672.2004.02388.x]
[6]
Bhoonobtong, A.; Sawadsitang, S.; Sodngam, S.; Mongkolthanaruk, W. Characterization of Endophytic bacteria, Bacillus amyloliquefaciens for antimicrobial agents production. Communication to the Int. Conf. Biol. Life Sci, 2012, (IACSIT Press, Singapore). 40, 6- 11.
[7]
Prieto, P.; Pineda, M.; Aguilar, M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phospho- molybdenum complex: Specific application to the determination of vitamin E. Anal. Biochem., 1999, 41, 269-337.
[http://dx.doi.org/10.1006/ abio.1999.4019]
[8]
Bauer, A.W.; Kirby, W.M.M.; Sherris, J.C.; Turck, M. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol., 1966, 6, 450-493.
[9]
Velho-Pereira, S.; Kamat, N. Antimicrobial screening of actinobacteria using a modified cross-streak method. Indian J. Pharm. Sci, 2011, 73(2), 223-228.
[http://dx.doi.org/10.4103/0250-474X. 91566.]
[10]
Patil, N.N.; Waghmode, M.S.; Gaikwad, P.S.; Gajbhai, M.H.; Bankar, A.V. Bioautography guided screening of antimicrobial compounds produced by Microbispora V2. Int. Res. J. Biol. Sci., 2013, 2(2), 65-68.
[11]
Tang, Z.; Qin, J.; Xu, X.; Shi, G.; Yang, H.; Liang, Y. Applying silica gel column chromatography to purify resveratrol from extracts of Morus alba L. Leaf. J. Med. Plants Res., 2011, 5(14), 3020-3027.
[12]
Nielsen, M.T.; Ranberg, J.A.; Christensen, U.; Christensen, H.B.; Harrison, S.J.; Olsen, C.E.; Hamberger, B.; Moller, B.L.; Norholm, M.H.H. Microbial synthesis of the forskolin precursor manoyl oxide in enantiomerically pure form. Appl. Environ. Microbiol., 2014, 80(23), 7258-7265.
[http://dx.doi.org/10.1128/AEM.02301-14]
[13]
Babushok, V.I.; Linstrom, P.J.; Zenkevich, I.G. Retention indices for frequently reported compounds of plant essential oils. J. Phys. Chem.Ref.Data, 2011, 40, 43101.
[http://dx.doi.org/10.1063/1.3653552]
[14]
Stepanchikova, A.V.; Lagunin, A.A.; Filimonov, D.V.; Poroikov, V.V. Prediction of biological activity spectra for substances: Evaluation on the diverse sets of drug like structures. Curr. Med. Chem., 2003, 10, 225-233.
[15]
Correa-Galeote, D.; Bedmar, E.J.; Arone, G.J. Maize endophytic bacterial diversity as affected by soil cultivation history. Front. Microbiol, 2018, 9, 484.
[http://dx.doi.org/10.3389/ fmicb.2018.00484.]
[16]
Minervini, F.; Celano, G.; Lattanzi, A.; Tedone, L.; De Mastro, G.; Gobbetti, M.; De Angelis, M. Lactic acid bacteria are endophytic components of durum wheat plant 2 following the whole life cycle from plant to flour. Appl. Environ. Microbiol., 2015, 81(19), 6736-6748.
[http://dx.doi.org/10.1128/AEM.01852-15]
[17]
Dhankhar, S.; Kumar, S.; Dhankhar, S.; Yadav, J.P. Antioxidant activity of fungal endophytes isolated from Salvadora oleoides Decne. Int. J. Pharm. Pharm. Sci., 2012, 4(2), 380-385.
[18]
Rhoden, S.A.; Garcia, A.; Bongiorno, V.A.; Azevedo, J.L.; Pamphile, J.A. Antimicrobial activity of crude extracts of endophytic fungi isolated from medicinal plant Trichilia elegans A. Juss. J. App. Pharm. Sci, 2013, 2(8), 57-59.
[http://dx.doi.org/10.7324/JAPS. 2012.21007.]
[19]
Basgedika, B.; Ugurb, A.; Saraca, N. Antimicrobial, antioxidant, and antimutagenic activities of Gladiolus illyricus. J. Pharm. Pharmacogn. Res., 2014, 2(4), 93-99.
[20]
Arya, P.; Sati, S.C. Evaluation of endophytic aquatic hyphomycetes for their antagonistic activity against pathogenic bacteria. Int. Res. J. Microbiol., 2011, 2(9), 343-347.
[21]
Ding, L.; Maier, A.; Fiebig, H-H.; Lin, W-H.; Hertweck, C.A. family of multicyclic indolosesquiterpenes from a bacterial endophyte. Org. Biomol. Chem., 2011, 9, 4029-4031.
[http://dx.doi.org/10.1039/c1ob05283g]
[22]
Taghavi, S.; van der Lelie, D.; Hoffman, A.; Zhang, Y.B.; Walla, M.D.; Vangronsveld, J.; Newman, L.; Monchy, S. Genome sequence of the plant growth promoting endophytic bacterium Enterobacter sp 638. PLoS Genet., 2010, 6 e1000943
[http://dx.doi.org/10.1371/journal.pgen.1000943]
[23]
Rodgman, A.; Perfetti, T.A. The chemical component of tobacco and tobacco smoke. 2ndEdn. CRC Press, Taylor and Francis Group: 6000 Broken Sound Parkway NW, Suite 300, Boca Raton, Florida. 33487-2742, Chapter 24 2013. pp. 1716, ISBN-13: 978-1-4665- 1552-9, 2013
[24]
Casella, S.; Leonardi, M.; Melai, B.; Fratini, F.; Pistelli, L. The role of diallyl sulphides and dipropylsulfides in the in vitro antimicrobial activity of the essential oil of garlic, Allium sativum L., and leek, Allium porrum L. Phytother. Res., 2013, 27, 380-383.
[http://dx.doi.org/10.1002/ptr.4725]

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