Abstract
Introduction: Fabaceae are a rich source of protease inhibitors.
Methods: A proteinaceous protease inhibitor of 25 KDa designated as Macrotyloma uniflorum protease inhibitor (MUPI) was isolated from seeds of Macrotyloma uniflorum and purified to homogeneity by ammonium sulphate precipitation, DEAE Sepharose column and CNBr activated Sepharose 4B Trypsin affinity chromatography. The purity was checked by reverse phase HPLC. Long-term type 2 diabetes can lead to various biological complications, such as hypertension and heart-related diseases. The glucose uptake studies were carried out with the purified MUPI and it reveals its potential to be explored as a potent anti-hyperglycemic agent. MUPI was rendered safer on HepG2 cells after MTT cytotoxicity assay.
Results: The results of glucose uptake studies suggest MUPI to be equally efficient to that of the positive control drug Metformin against diabetes mellitus.
Conclusion: The significance of plant-based inhibitors for modulating carbohydrate breakdown and control of glycemic index is explored to reduce the risk factors and side effects of the available drugs.
Keywords: Protease inhibitor, Glucose uptake, Macrotyloma uniflorum, Purification, Characterization, Degradation.
Current Topics in Medicinal Chemistry
Title:Anti-hyperglycemic Properties of a Purified Proteinaceous Protease Inhibitor from Macrotyloma Uniflorum Seeds
Volume: 18 Issue: 29
Author(s): Manju Mohan and Kothanam K. Elyas*
Affiliation:
- Department of Biotechnology, University of Calcutta, 673635, Kerala,India
Keywords: Protease inhibitor, Glucose uptake, Macrotyloma uniflorum, Purification, Characterization, Degradation.
Abstract: Introduction: Fabaceae are a rich source of protease inhibitors.
Methods: A proteinaceous protease inhibitor of 25 KDa designated as Macrotyloma uniflorum protease inhibitor (MUPI) was isolated from seeds of Macrotyloma uniflorum and purified to homogeneity by ammonium sulphate precipitation, DEAE Sepharose column and CNBr activated Sepharose 4B Trypsin affinity chromatography. The purity was checked by reverse phase HPLC. Long-term type 2 diabetes can lead to various biological complications, such as hypertension and heart-related diseases. The glucose uptake studies were carried out with the purified MUPI and it reveals its potential to be explored as a potent anti-hyperglycemic agent. MUPI was rendered safer on HepG2 cells after MTT cytotoxicity assay.
Results: The results of glucose uptake studies suggest MUPI to be equally efficient to that of the positive control drug Metformin against diabetes mellitus.
Conclusion: The significance of plant-based inhibitors for modulating carbohydrate breakdown and control of glycemic index is explored to reduce the risk factors and side effects of the available drugs.
Export Options
About this article
Cite this article as:
Mohan Manju and Elyas K. Kothanam*, Anti-hyperglycemic Properties of a Purified Proteinaceous Protease Inhibitor from Macrotyloma Uniflorum Seeds, Current Topics in Medicinal Chemistry 2018; 18 (29) . https://dx.doi.org/10.2174/1568026619666181220110038
DOI https://dx.doi.org/10.2174/1568026619666181220110038 |
Print ISSN 1568-0266 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4294 |
Call for Papers in Thematic Issues
Chemistry Based on Natural Products for Therapeutic Purposes
The development of new pharmaceuticals for a wide range of medical conditions has long relied on the identification of promising natural products (NPs). There are over sixty percent of cancer, infectious illness, and CNS disease medications that include an NP pharmacophore, according to the Food and Drug Administration. Since NP ...read more
Current Trends in Drug Discovery Based on Artificial Intelligence and Computer-Aided Drug Design
Drug development discovery has faced several challenges over the years. In fact, the evolution of classical approaches to modern methods using computational methods, or Computer-Aided Drug Design (CADD), has shown promising and essential results in any drug discovery campaign. Among these methods, molecular docking is one of the most notable ...read more
Drug Discovery in the Age of Artificial Intelligence
In the age of artificial intelligence (AI), we have witnessed a significant boom in AI techniques for drug discovery. AI techniques are increasingly integrated and accelerating the drug discovery process. These developments have not only attracted the attention of academia and industry but also raised important questions regarding the selection ...read more
From Biodiversity to Chemical Diversity: Focus of Flavonoids
Flavonoids are the largest group of polyphenols, plant secondary metabolites arising from the essential aromatic amino acid phenylalanine (or more rarely from tyrosine) via the phenylpropanoid pathway. The flavan nucleus is the basic 15-carbon skeleton of flavonoids (C6-C3-C6), which consists of two phenyl rings (A and B) and a heterocyclic ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Mechanisms, Prevention and Treatment of Distal Embolization
Current Pharmaceutical Design Safety of Cabergoline in the Management of Pituitary Prolactin-Induced Symptoms with Patients Treated with Atypical Neuroleptics
Current Drug Safety Mitochondrially Targeted Antioxidants for the Treatment of Cardiovascular Diseases
Recent Patents on Cardiovascular Drug Discovery Editorial [ Endothelial Dysfunction: Novel Therapeutic Approaches (Guest Editor: Dimitris Tousoulis)]
Current Vascular Pharmacology Aortic Stenosis, Aortic Regurgitation and Arterial Hypertension
Current Vascular Pharmacology Adenosine A<sub>2A</sub> Receptor Antagonists as Positron Emission Tomography (PET) Tracers
Current Medicinal Chemistry Cell-free DNA: Characteristics, Detection and its Applications in Myocardial Infarction
Current Pharmaceutical Design Role of PPAR-γ Agonist Thiazolidinediones in Treatment of Pre-Diabetic and Diabetic Individuals: A Cardiovascular Perspective
Current Drug Targets - Cardiovascular & Hematological Disorders Trace and Minor Element Concentrations in Aqueous Extract of the Herbal Medicinal Plants Tamarindus indica and Hibiscus sabdiffora
Current Nutrition & Food Science Glycoxidation and Wound Healing in Diabetes: An Interesting Relationship
Current Diabetes Reviews Editorial (Thematic Issue: Therapies Targeting Oxidative Stress for Human Diseases: Where Are We Now?)
Current Pharmaceutical Biotechnology Clinical Science of Calcium Channel Blocker to Inhibit Hypertensive Vascular Injury
Current Hypertension Reviews "Heart Failure: Meeting the Challenges of Surveillance and Knowledge Translation in Resource-poor Settings"
Current Cardiology Reviews Recent Advances in Permeation Enhancement Techniques for Transdermal Drug Delivery Systems: A Review
Current Drug Therapy Stroke and Hypertension: An Appraisal from Pathophysiology to Clinical Practice
Current Vascular Pharmacology Statins and Inflammation in Cardiovascular Disease
Current Pharmaceutical Design Differential Regulation of microRNAs in Patients with Ischemic Stroke
Current Neurovascular Research Arterial Hypertension and Multiple Myeloma: Physiopathology and Cardiovascular Risk and ‘Practical’ Indications in Patients Receiving Carfilzomib
Current Hypertension Reviews Psoriasis and Atherosclerosis: Is There a Need For Novel Biomarkers Assessing Cardiovascular Risk?
Current Pharmaceutical Design Migraine: Pathophysiology, Pharmacology, Treatment and Future Trends
Current Vascular Pharmacology