The goal of the review is description of the main characteristics
of creatinine deiminase (CDI), an important bioanalytical tool for
creatinine (Crn) assay. Crn is an essential metabolite for diagnostics of
kidney disfunction and some other diseases, a biomarker to control the
hemodialysis procedure, as well as an important analyte for sport medicine
(estimation of general physiological status of athletes). We have described
the important sources for CDI isolation, cloning of the corresponding gene,
the construction of microbial recombinant strains, overproducing CDI, and
characteristics of the enzyme from different microorganisms. There are
reviewing also the new bioanalytical methods for quantitative determination
of Crn, including enzymatic ones based on using CDI.
Keywords: creatinine deiminase, protein characterization, gene cloning,
enzyme isolation, creatinine, creatinine enzymatic assay.
Rights & PermissionsPrintExport