Abstract
Purpose: Ultrasound contrast agents involving a therapeutic drug applied during enhanced imaging can be used for targeted therapy. The preparation of contrast agents is a precondition and basis for the use of multifunctional contrast agents in molecular imaging.
Methods: This study uses thin-film hydration-mechanical vibration to carry out the preliminary preparation of a cationic contrast agent (CCA); characterizes the particle diameter, potential, distribution, and concentration of the agents; and optimizes the factors affecting the preparation of the agents.
Results: This study found that thin-film hydration-mechanical vibration methods offer a better preparation effectiveness and achieve smaller particle diameters and more even distributions, as well as give better imaging performance. Different concentrations of CCA and plasmid and different gene transfection methods can produce different degrees of sonoporation to achieve optimal transfection efficiency. Ultrasound parameters have a great influence on transfection efficiency and plasmid integrity. A previous study confirmed that the ultrasound parameters of 1 MHz, 1 W/cm2, a duty cycle controller (DC) of 20%, and irradiation for 1 min can well deliver genes to tumor cells, with little impact on cell survival.
Conclusion: Our findings indicate that ultrasound-targeted microbubble destruction (UTMD)-mediated CCA destruction facilitates gene transfection and may represent an effective gene delivery method for cervical cancer therapy.
Keywords: Ultrasound contrast agent, cationic, gene transfection, thin-film hydration method, Cationic Contrast Agent (CCA), Ultrasoundtargeted Microbubble Destruction (UTMD).
Current Pharmaceutical Design
Title:Preparation of Cationic Lipid-coated Ultrasound Contrast Agents and Noninvasive Gene Transfection Via Ultrasound-targeted Microbubble Destruction
Volume: 24 Issue: 30
Author(s): Feng Yang, Yue Li, Chun Liufu, Yi Wang and ZhiYi Chen*
Affiliation:
- Department of Ultrasound Medicine, Laboratory of Ultrasound Molecular Imaging, The Third Affiliated Hospital of Guangzhou Medical University, The Liwan Hospital of the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, 510000,China
Keywords: Ultrasound contrast agent, cationic, gene transfection, thin-film hydration method, Cationic Contrast Agent (CCA), Ultrasoundtargeted Microbubble Destruction (UTMD).
Abstract: Purpose: Ultrasound contrast agents involving a therapeutic drug applied during enhanced imaging can be used for targeted therapy. The preparation of contrast agents is a precondition and basis for the use of multifunctional contrast agents in molecular imaging.
Methods: This study uses thin-film hydration-mechanical vibration to carry out the preliminary preparation of a cationic contrast agent (CCA); characterizes the particle diameter, potential, distribution, and concentration of the agents; and optimizes the factors affecting the preparation of the agents.
Results: This study found that thin-film hydration-mechanical vibration methods offer a better preparation effectiveness and achieve smaller particle diameters and more even distributions, as well as give better imaging performance. Different concentrations of CCA and plasmid and different gene transfection methods can produce different degrees of sonoporation to achieve optimal transfection efficiency. Ultrasound parameters have a great influence on transfection efficiency and plasmid integrity. A previous study confirmed that the ultrasound parameters of 1 MHz, 1 W/cm2, a duty cycle controller (DC) of 20%, and irradiation for 1 min can well deliver genes to tumor cells, with little impact on cell survival.
Conclusion: Our findings indicate that ultrasound-targeted microbubble destruction (UTMD)-mediated CCA destruction facilitates gene transfection and may represent an effective gene delivery method for cervical cancer therapy.
Export Options
About this article
Cite this article as:
Yang Feng , Li Yue, Liufu Chun , Wang Yi and Chen ZhiYi *, Preparation of Cationic Lipid-coated Ultrasound Contrast Agents and Noninvasive Gene Transfection Via Ultrasound-targeted Microbubble Destruction, Current Pharmaceutical Design 2018; 24 (30) . https://dx.doi.org/10.2174/1381612824666181011120031
DOI https://dx.doi.org/10.2174/1381612824666181011120031 |
Print ISSN 1381-6128 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4286 |
Call for Papers in Thematic Issues
"Tuberculosis Prevention, Diagnosis and Drug Discovery"
The Nobel Prize-winning discoveries of Mycobacterium tuberculosis and streptomycin have enabled an appropriate diagnosis and an effective treatment of tuberculosis (TB). Since then, many newer diagnosis methods and drugs have been saving millions of lives. Despite advances in the past, TB is still a leading cause of infectious disease mortality ...read more
Current Pharmaceutical challenges in the treatment and diagnosis of neurological dysfunctions
Neurological dysfunctions (MND, ALS, MS, PD, AD, HD, ALS, Autism, OCD etc..) present significant challenges in both diagnosis and treatment, often necessitating innovative approaches and therapeutic interventions. This thematic issue aims to explore the current pharmaceutical landscape surrounding neurological disorders, shedding light on the challenges faced by researchers, clinicians, and ...read more
Emerging and re-emerging diseases
Faced with a possible endemic situation of COVID-19, the world has experienced two important phenomena, the emergence of new infectious diseases and/or the resurgence of previously eradicated infectious diseases. Furthermore, the geographic distribution of such diseases has also undergone changes. This context, in turn, may have a strong relationship with ...read more
Melanoma and Non-Melanoma Skin Cancer Treatment: Standard of Care and Recent Advances
In this thematic issue, we aim to provide a standard of care of the diagnosis and treatment of melanoma and non-melanoma skin cancer. The editor will invite authors from different countries who will write review articles of melanoma and non-melanoma skin cancers. The Diagnosis, Staging, Surgical Treatment, Non-Surgical Treatment all ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Aging and Systemic Lupus Erythematosus - Immunosenescence and Beyond
Current Aging Science The CCL2/CCR2 Axis in the Pathogenesis of HIV-1 Infection: A New Cellular Target for Therapy?
Current Drug Targets Recent Developments Towards the Synthesis of Varitriol: An Antitumour Agent from Marine Derived Fungus Emericella Variecolor
Current Organic Synthesis Exploring the Bio-efficacies of Methanolic Extracts of Nostoc muscorum and Calothrix brevissima with their Characterization Using GC-MS
The Natural Products Journal Prostate Cancer Prevention in the Developing World - What are we Waiting for?
Current Pharmacogenomics and Personalized Medicine Malignancy and Radiation-Induced Cardiotoxicity
Cardiovascular & Hematological Disorders-Drug Targets NF-κB Inhibitors in Head and Neck Cancer
Letters in Drug Design & Discovery An update on Anti-inflammatory Compounds: A Review
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Non-steroidal Anti-Inflammatory Drugs and Melanoma
Current Pharmaceutical Design Recent Advances of Chitosan and its Derivatives in Biomedical Applications
Current Medicinal Chemistry Therapeutic Applications of Crocus sativus L. (Saffron): A Review
The Natural Products Journal Overview of Current Immunotherapies Targeting Mutated KRAS Cancers
Current Topics in Medicinal Chemistry Allosteric Inhibition of G-Protein Coupled Receptor Oligomerization: Strategies and Challenges for Drug Development
Current Topics in Medicinal Chemistry Targeting the Akt/PI3K Signaling Pathway as a Potential Therapeutic Strategy for the Treatment of Pancreatic Cancer
Current Medicinal Chemistry Cucurbitacin IIb from Ibervillea sonorae Induces Apoptosis and Cell Cycle Arrest via STAT3 Inhibition
Anti-Cancer Agents in Medicinal Chemistry Thyroid Cancer and Nodules in Graves’ Disease: A Single Center Experience
Endocrine, Metabolic & Immune Disorders - Drug Targets Benzothiazole Derivatives: Novel Inhibitors of Methylglyoxal Mediated Glycation of Proteins In Vitro
Medicinal Chemistry Melanoma Immunotherapy: Past, Present, and Future
Current Pharmaceutical Design The Clinical Applications of Heat Shock Protein Inhibitors in Cancer - Present and Future
Current Cancer Drug Targets Surgical Strategies for Fertility Preservation in Women with Cancer
Current Women`s Health Reviews