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Current Bioactive Compounds

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ISSN (Print): 1573-4072
ISSN (Online): 1875-6646

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Research Article

Effects of Greek Pomegranate Extracts in the Antioxidant Properties and Storage Stability of Kefir

Author(s): Vasiliki Lagouri*, Georgia Dimitreli and Aikatarini Kouvatsi

Volume 15, Issue 4, 2019

Page: [437 - 441] Pages: 5

DOI: 10.2174/1573407214666180808113450

Price: $65

Abstract

Background: Oxidation reactions are known to shorten the shelf life and cause damage to foods rich in fat, such as dairy products. One way to limit oxidation and increase the shelf life of fermented dairy products is to use natural antioxidants. The aim of this study was to examine the effect of adding pomegranate extracts in the antioxidant properties, rheological characteristics and the storage stability of the fermented product of kefir.

Methods: The Pomegranate Juice (PGJ) and Peel Extracts (PGPE) (5%, 10% w/v) were added to kefir and the antioxidant properties were evaluated by using the methods of radical scavenging activity (DPPH) and Ferric Reducing Antioxidant Power Activity (FRAP). Spectrophotometric and instrumental methods were used to determine the Total Phenols (TPs), pH values, viscosity and flow behavioral index values of enriched with pomegranate kefir samples. The same properties were tested when kefir samples stored at 4°C for 7, 14, 21 and 28 days.

Results: The addition of PGJ and PGPE results in an increase in the antioxidant activity (DPPH, FRAP) and total phenol content (TPs) of kefir samples. Increasing the concentration of the added PGJ and PGPE, results in an increase in the TP content and the DPPH activity of kefir. As far as the storage time is concerned, the results showed an increase in the amount of TP at 7th day and a reduction in the DPPH activity in the 14th day of storage. In contrary to the DPPH method, the increase in storage time has resulted in a reduction in antioxidant activity by the FRAP method. The addition of PGJ and PGPE in kefir results in a decrease in pH values while the pH of kefir samples increased during storage at 4°C for 28 days. The addition of PGJ and PGPE to kefir samples results to a decrease in viscosity and an increase in the flow behavior index. Increasing storage time results in increased flow behavior index of kefir samples.

Conclusion: The addition of PGJ and PGPE increased the antioxidant activity and total phenols of the kefir product and preserved its properties during the total storage time of 28 days at 4°C.

Keywords: Pomegranate, kefir, dairy products, phenols, storage, rheological properties, antioxidant activity.

Graphical Abstract

[1]
Halliwell, B.; Gutteridge, J.M.C.; Cross, C.E. Free radicals, antioxidants, and human disease: Where are we now? J. Lab. Clin. Med., 1992, 119(6), 598-620.
[2]
Scalbert, A.; Manach, C.; Morand, C.; Rémésy, C.; Jiménez, L. Dietary polyphenols and the prevention of diseases. Crit. Rev. Food Sci. Nutr., 2005, 45(4), 287-306.
[3]
Li, Y.; Guo, C.; Yang, J.; Wei, J.; Xu, J.; Cheng, S. Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chem., 2006, 96, 254-260.
[4]
Negi, P.S.; Jayaprakasha, G.K.; Jena, B.S. Antioxidant and antimutagenic activities of pomegranate peel extracts. Food Chem., 2003, 80(3), 393-397.
[5]
Lansky, E.P.; Newman, R.A. Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. J. Ethnopharmacol., 2007, 109(2), 177-206.
[6]
Siti, M.M.; Lee, S.C.; Hesham, A. El- Enshasy; Fadzilah A.A.; Majid, Roslinda A. Malek. A Review on Fruit Juice Probiotication: Pomegranate. Curr. Nutr. Food Sci., 2016, 12(1), 4-11.
[7]
Seeram, N.P.; Adamsa, L.S.; Henninga, S.M.; Niu, Y.; Zhang, Y.; Nair, M.G.; Heber, D. In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin pomegranate juice. J. Nutr. Biochem., 2005, 16(6), 360-367.
[8]
Vattem, D.A.; Shetty, K. Biological functionality of ellagic acid: A review. J. Food Biochem., 2005, 29(3), 234-266.
[9]
Gil, M.I.; Tomás-Barberán, F.A.; Hess-Pierce, B.; Holcroft, D.M.; Kader, A.A. Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. J. Agric. Food Chem., 2000, 48(10), 4581-4589.
[10]
Tzulker, R.; Glazer, I.; Bar-Ilan, I.; Holland, D.; Aviram, M.; Amir, R. Antioxidant activity, polyphenol content, and related compounds in different fruit juices and homogenates prepared from 29 different pomegranate accessions. J. Agric. Food Chem., 2007, 55(23), 9559-9570.
[11]
Tezcan, F.; Gültekin, Ö.M.; Diken, T.; Özçelik, B.; Bedia, E.F. Antioxidant activity and total phenolic, organic acid and sugar content in commercial pomegranate juices. Food Chem., 2009, 115(3), 873-877.
[12]
Singh, R.P.; Chidambara Murthy, K.N.; Jayaprakasha, G.K. Studies on the antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitro models. J. Agric. Food Chem., 2002, 50(1), 81-86.
[13]
Li, Y.; Guo, C.; Yang, J.; Wei, J.; Xu, J.; Cheng, S. (). Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chem., 2006, 96(2), 254-260.
[14]
Çam, M.; Yasar, H. Pressurised water extraction of polyphenols from pomegranate peels. Food Chem., 2010, 123(3), 878-885.
[15]
Garrote, G.; Abraham, A.; De Antoni, G. Characteristics of kefir prepared with different grain: Milk ratios. J. Dairy Res., 1998, 65(1), 149-154.
[16]
Beshkova, D.M.; Simona, E.D.; Simon, Z.I.; Frengova, G.I.; Spasov, Z.I. Pure cultures for making kefir. Food Microbiol., 2002, 19(5), 537-544.
[17]
Otles, S.; Cadingi, O. Kefir: A probiotic dairy-composition, nutritional and therapeutic aspects. Pak. J. Nutr., 2003, 2(2), 54-59.
[18]
Witthuhn, R.C.; Schoeman, T.; Britz, T.J. Isolation and characterization of the microbial population of different South African Kefir grains. Int. J. Dairy Technol., 2004, 57(1), 33-37.
[19]
Irigoyen, A.; Arama, I.; Castiella, M.; Torre, P.; Ibanez, F.C. Microbiological, physicochemical, and sensory characteristics of kefir during storage. Food Chem., 2005, 90(4), 613-620.
[20]
Carmen Garcia-Martinez, M. gloria marquez-ruiz. lipid oxidation in functional dairy products. Curr. Nutr. Food Sci., 2009, 5(3), 209-216.
[21]
Brand-Williams, W.; Cuvelier, M.E.; Berset, C. Use of free radical method to evaluate antioxidant activity. Lebensm. Wiss. Technol., 1995, 28(1), 25-30.
[22]
Benzie, I.F.; Strain, J.J. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal. Biochem., 1996, 239(1), 70-76.
[23]
Slinkard, K.; Singleton, V.L. Total phenol analysis: automation and comparison with manual methods. Am. J. Enol. Vitic., 1997, 28(1), 49-55.
[24]
Amirdivani, S.; Baba, S. Changes in yogurt fermentation characteristics and antioxidant potential and in vitro inhibition of angiotensin-1 converting enzyme upon the inclusion of peppermint, dill and basil. Lebensm. Wiss. Technol., 2011, 44(11), 1458-1464.
[25]
Salaȕn, F.; Mietton, B.; Gaucheron, F. Buffering capacity of dairy products. Int. Dairy J., 2005, 15(2), 95-109.
[26]
Koroleva, N.S. Products prepared with lactic acid bacteria and yeasts.Therapeutic properties of fermented milks; Robinson, R.K., Ed.; Elsevier Applied Sciences Publishers: London, 1991, pp. 159-179.
[27]
Walstra, P.; Wouters, J.T.M.; Geurts, T.J. Dairy Science and Technology, 2nd ed; CRC (Food science and technology 147): CRC Press: Boca Raton, FL, USA,, 2005, p. 782..

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