Adjuvant Activity of Bursal Pentapeptide-(III-V) in Mice Immunized with the H9N2 Avian Influenza Vaccine

Title:Adjuvant Activity of Bursal Pentapeptide-(III-V) in Mice Immunized with the H9N2 Avian Influenza Vaccine

Volume: 25 Issue: 8

Author(s): Jiangfei Zhou, Cong Zhang, Zhixin Liu, Yongqing Liu, Kairui Cai, Tengfei Shen, Chengshui Liao*Chen Wang*

Affiliation:

• College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan,China

• College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan,China

Keywords: Bursal pentapeptided-(III-V), adjuvants, humoral immune response, cellular immune response, immunoprotection test, avian influenza virus.

Abstract: Background: Bursal Pentapeptide (BPP)-(III-V) are peptides isolated from the Bursa of Fabricius (BF).

Method: The peptides' adjuvant activities were evaluated in mice immunized with the H9N2 avian influenza vaccine.

Results: The results showed that BPP-IV did not promote humoral or cellular immune responses. BPP-III had no effect on antibody production, but increased populations of CD3+ and CD8+ T cells, CTL levels and proliferation activities of splenic T-lymphocytes. BPP-V increased the level of antibodies (HI, IgG and its subtype IgG1) and the cytokine IL-4, as well as the ratio of CD3+ T cells and its subsets CD4+ and CD8+ T cells, BPP-V also increased CTL level and promoted the proliferation activity of T- and B-lymphocyte. Furthermore, in animal challenge experiment, BPP-III and BPP-V reduced viral RNA copies and viral titers, and reduced inflammatory cell infiltration.

Conclusion: In summary, BPP-IV did not have an effect on specific immune responses, therefore it could not reduce pathological damage in mice lungs; BPP-III provided resistance to the antigen through the cell-mediated immune response; BPP-V enhanced both humoral and cellular immune responses, therefore provided protections against the H9N2 Avian Influenza Virus (AIV). Altogether, BPP-III and BPP-V could be adjuvant candidates for the H9N2 avian influenza vaccine.

Cite this article as:

Zhou Jiangfei , Zhang Cong , Liu Zhixin , Liu Yongqing, Cai Kairui , Shen Tengfei , Liao Chengshui *, Wang Chen*, Adjuvant Activity of Bursal Pentapeptide-(III-V) in Mice Immunized with the H9N2 Avian Influenza Vaccine, Protein & Peptide Letters 2018; 25 (8) . https://dx.doi.org/10.2174/0929866525666180806110928