LncRNA MALAT1 is Neuroprotective in a Rat Model of Spinal Cord Ischemia-Reperfusion Injury Through miR-204 Regulation

Title:LncRNA MALAT1 is Neuroprotective in a Rat Model of Spinal Cord Ischemia-Reperfusion Injury Through miR-204 Regulation

Volume: 15 Issue: 3

Author(s): Yong Qiao, Changliang Peng, Ji Li, Dongjin Wu and Xiuwen Wang*

Affiliation:

• Department of Spinal Surgery, The Second Hospital of Shandong University, Ji`nan,China

Keywords: MALAT-1, miR-204, spinal cord, ischemia/reperfusion injury, oxygen, glucose deprivation.

Abstract: Background: This study was to investigate the neuroprotective effect of long noncoding RNAs Metastasis associated lung adenocarcinoma transcript-1 (lncRNA MALAT-1) in Spinal Cord Ischemic/Reperfusion Injury (SCIRI).

Methods: Quantitative real-time PCR (RT-qPCR) was used to examine the expressions of MALAT1, miR-204 and Bcl-2, while western blot was performed to examine Bcl-2. Besides, apoptosis was evaluated by the percentage of cell viability and apoptotic cells. Neurological evaluation was performed by measuring hindlimb locomotor function.

Results: The expression of MALAT1 and Bcl-2 was decreased, while miRNA-204 (miR-204) was up-regulated in rats SCIRI model and neurocyte lines under hypoxic conditions. Oxygen, Glucose Deprivation (OGD) promoted apoptosis of neurocytes, downregulated MALAT1 and Bcl-2 and elevated miR-204 expression, however, overexpression of MALAT1 notably reversed this trend. Nevertheless, knockdown of MALAT1 increased cell apoptosis, decreased MALAT1 and Bcl-2 but upregulated miR-204. MALAT1 overexpression-induced anti-apoptosis and knockdowninduced pro-apoptosis were obviously reversed by synchronously overexpression and knockdown of miR-204, respectively. MALAT1-treated SCIRI rats exhibited lower Motor Deficit Index (MDI) scores, higher levels of MALAT1 and Bcl-2 expression and lower miR-204 expression.

Conclusion: Our data suggested that MALAT1 exerted neuroprotective effect in a rat model of SCIRI by regulating miR-204.

Cite this article as:

Qiao Yong , Peng Changliang, Li Ji , Wu Dongjin and Wang Xiuwen *, LncRNA MALAT1 is Neuroprotective in a Rat Model of Spinal Cord Ischemia-Reperfusion Injury Through miR-204 Regulation, Current Neurovascular Research 2018; 15 (3) . https://dx.doi.org/10.2174/1567202615666180712153150