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Letters in Drug Design & Discovery

Editor-in-Chief

ISSN (Print): 1570-1808
ISSN (Online): 1875-628X

Research Article

The Impact of Antioxidants from the Diet on Breast Cancer Cells Monitored by Raman Microspectroscopy

Author(s): Paula Sofia Coutinho Medeiros, Ana Lúcia Marques Batista de Carvalho*, Cristina Ruano, Juan Carlos Otero and Maria Paula Matos Marques

Volume 16, Issue 2, 2019

Page: [127 - 137] Pages: 11

DOI: 10.2174/1570180815666180502120804

Price: $65

Abstract

Background: The impact of the ubiquitous dietary phenolic compound p-coumaric acid on human breast cancer cells was assessed, through a multidisciplinary approach: Combined biological assays for cytotoxicity evaluation and biochemical profiling by Raman microspectroscopic analysis in cells.

Methods: Para-coumaric acid was shown to exert in vitro chemoprotective and antitumor activities, depending on the concentration and cell line probed: a significant anti-invasive ability was detected for the triple-negative MDA-MB-231 cells, while a high pro-oxidant effect was found for the estrogen- dependent MCF-7 cells. A striking cell selectivity was obtained, with a more noticeable outcome on the triple-negative MDA-MB-231 cell line.

Results: The main impact on the cellular biochemical profile was verified to be on proteins and lipids, thus justifying the compound´s anti-invasive effect and chemoprotective ability.

Conclusion: p-Coumaric acid was thus shown to be a promising chemoprotective/chemotherapeutic agent, particularly against the low prognosis triple-negative human breast adenocarcinoma.

Keywords: Dietary antioxidants, p-coumaric acid, breast cancer cells, chemoprotective/chemotherapeutic, raman microspectroscopy, antitumor activities.

Graphical Abstract
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