Flow Cytometric Analysis of Pro-inflammatory Cytokine Production in Hyperglycemic Mouse Model

Title:Flow Cytometric Analysis of Pro-inflammatory Cytokine Production in Hyperglycemic Mouse Model

Volume: 9 Issue: 2

Author(s): Muhaimin Rifa`i*, Mulya D. Wahyuningsih, Noviana D. Lestari, Aris Soewondo, Mansur Ibrahim and Hideo Tsuboi

Affiliation:

• Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran Malang 65145, East Java,Indonesia

Keywords: Hyperglycemia, Muntingia calabura L, cytokine, pro-inflammatory, physiological processes, pathogenicity.

Abstract: Objective: The sharp increase of pro-inflammatory cytokine becomes a severe problem in some degenerative diseases. This study aimed to investigate the effect of Cherry Leaf Extract (CLE) on pro-inflammatory cytokine production in hyperglycaemic mouse model. A recent patent mentioned that Muntinga calabura is a flavonoid-containing plant which is capable of reinforcing the stomach, appetizing, helping digestion (CN 201611138374), and improving stomach distention (CN106071017A). The efficacy of this miracle plant has inspired this experiment.

Methods: In this study, we induced hyperglycemia with high-fat diet and 20% fructose drink. Hyperglycemic mice were orally administered with CLE with the doses 420, 700, and 28000 mg/kg BW for two weeks. After treatment, the pro-inflammatory molecule was analyzed by flow cytometry from splenic cells.

Results: This study showed that CLE decreased the production of pro-inflammatory molecules such as IL-6, NF-κB, TNF-α, and IFN γ. In hyperglycemic mice, we found CD68+IL-6+, NK1.1+IL- 6+, B220+NFκB+, CD4+TNF-α+, and CD4+IFN γ + increased significantly (p < 0.05) compared to control mice without manipulation. After treatment with 420, 700, or 2800 mg/kg BW of CLE, the pro-inflammatory molecules decreased significantly (p < 0.05), and reached a normal physiology level.

Conclusion: CLE can suppress pro-inflammatory cytokines in hyperglycemic mouse model, suggesting this medicinal herb may be beneficial as a potential strategy for future therapeutic interventions in degenerative diseases or a disease involving cell activation.

Cite this article as:

Rifa`i Muhaimin *, Wahyuningsih D. Mulya , Lestari D. Noviana , Soewondo Aris , Ibrahim Mansur and Tsuboi Hideo , Flow Cytometric Analysis of Pro-inflammatory Cytokine Production in Hyperglycemic Mouse Model, Recent Patents on Food, Nutrition & Agriculture 2018; 9 (2) . https://dx.doi.org/10.2174/2212798410666180426161016