Class II hydrophobin HFBII is a fungal protein with potential applications in pharmaceutical
industry. Nevertheless, the antitumor activity of this protein has not been reported. In this study, a natural
type of Trichoderma reesei was cultivated in a submerged bioreactor to produce hydrophobin HFBII.
The protein was purified using a reversed phase liquid chromatography, verified by MALDI-TOF, and
then examined for its anticancer activity against T47D breast tumor cell line. Different concentrations of
hydrophobin HFBII from 1 nM to 500 μM were examined for this experiment. Hydrophobin HFBII
with molecular weights of 7.0-7.2 kDa was achieved. The results of MTT assay showed that from the
concentration of 100 μM and upwards, hydrophobin HFBII adversely affected the viability of the breast
tumor cells. The IC50 value was reported to be 131 μM. Class II hydrophobin seems to be a very effective
carrier for antitumor agent.
Keywords: HFBII, hydrophobin, Trichoderma reesei, drug delivery, antitumor
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