Abstract
Background: Considering the economic loss by columnaris disease in fish, a rapid, simpleto- use and visual detection technology for the detection of Flavobacterium columnare (F. columnare) is very important.
Methods: This assay utilizes LAMP for F. columnare-specific target gene amplification, and the resulting LAMP amplicons were further analyzed by Immunochromatographic Strip (ICS) for the rapid visual detection of F. columnare. The performance of such method includeing specificity, sensitivity, repeatability and practical use were validated.
Results: This assay demonstrated high specificity, repeatability and has a limit of detection of 2.5 × 102 F. columnare CFU. The reliability of this assay was further confirmed by the equencing and alignment of LAMP amplicon with the DNA sequence of F. columnare. The whole detection process was less than 50 min.
Conclusion: The LAMP-ICS assay presented here is simple, rapid, and reliable. Moreover, it could be easily adapted to detect other fish pathogens, and it may be more suitable for future use in the resourcelimit region for the detection of fish pathogen on site.
Keywords: Loop mediated isothermal amplification, immunochromatographic strip, Flavobacterium columnare, fish pathogen, visual detection, PCR.
Current Analytical Chemistry
Title:Loop Mediated Isothermal Amplification Combining Immunochromatographic Strip for Point-of-care Detection of Flavobacterium columnare
Volume: 14 Issue: 5
Author(s): Yong-Jin Li*, Yingying Yang, Sisi Zheng and Jin-Yu Fan
Affiliation:
- College of Life Science, Huzhou University, Huzhou 313000,China
Keywords: Loop mediated isothermal amplification, immunochromatographic strip, Flavobacterium columnare, fish pathogen, visual detection, PCR.
Abstract: Background: Considering the economic loss by columnaris disease in fish, a rapid, simpleto- use and visual detection technology for the detection of Flavobacterium columnare (F. columnare) is very important.
Methods: This assay utilizes LAMP for F. columnare-specific target gene amplification, and the resulting LAMP amplicons were further analyzed by Immunochromatographic Strip (ICS) for the rapid visual detection of F. columnare. The performance of such method includeing specificity, sensitivity, repeatability and practical use were validated.
Results: This assay demonstrated high specificity, repeatability and has a limit of detection of 2.5 × 102 F. columnare CFU. The reliability of this assay was further confirmed by the equencing and alignment of LAMP amplicon with the DNA sequence of F. columnare. The whole detection process was less than 50 min.
Conclusion: The LAMP-ICS assay presented here is simple, rapid, and reliable. Moreover, it could be easily adapted to detect other fish pathogens, and it may be more suitable for future use in the resourcelimit region for the detection of fish pathogen on site.
Export Options
About this article
Cite this article as:
Li Yong-Jin*, Yang Yingying , Zheng Sisi and Fan Jin-Yu, Loop Mediated Isothermal Amplification Combining Immunochromatographic Strip for Point-of-care Detection of Flavobacterium columnare, Current Analytical Chemistry 2018; 14 (5) . https://dx.doi.org/10.2174/1573411014666171220145220
DOI https://dx.doi.org/10.2174/1573411014666171220145220 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Analytical methods for environmental and food analysis
In recent years, the study of the chemical composition of food and the environment depends on analytical methods in order to acquire information about the chemical composition, processing, quality control and contamination of soil, water, air, plants and foods, aspects very important for food safety and environmental quality control. There ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
Bioanalytical and Biosensor Technologies for Target Molecules
Bioanalytical and biosensor technologies constitute the core of detecting and sensing diverse molecules within living organisms, encompassing proteins, DNA, small molecular metabolites, and beyond. These molecules assume pivotal roles in biological processes, rendering their analysis and sensing indispensable for comprehending biological phenomena and unraveling disease mechanisms. In the current landscape ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Bioactive Albumin-Based Carriers for Tumour Chemotherapy
Current Cancer Drug Targets Anti-Tubercular Agents from <i>Glycyrrhiza</i> glabra
Current Topics in Medicinal Chemistry An 8-Year Retrospective Study of Human Visceral Leishmaniasis
Current Clinical Pharmacology Synthesis and Biological Evaluation of Naphthalene-1,4-dione Derivatives as Potent Antimycobacterial Agents
Medicinal Chemistry Biotechnological Potential of <i>Streptomyces</i> Siderophores as New Antibiotics
Current Medicinal Chemistry Editorial [Tuberculosis Drugs and Drug Targets]
Infectious Disorders - Drug Targets Antipsoriatic Drug Development: Challenges and New Emerging Therapies
Recent Patents on Inflammation & Allergy Drug Discovery Secondary Metabolites from Cordyceps Species and Their Antitumor Activity Studies
Recent Patents on Biotechnology HIV and Respiratory Disease: A Contemporary Perspective
Current Respiratory Medicine Reviews Coumarin-Oxadiazole Derivatives: Synthesis and Pharmacological Properties
Mini-Reviews in Organic Chemistry Immunomodulation Mechanism of Antidepressants: Interactions between Serotonin/Norepinephrine Balance and Th1/Th2 Balance
Current Neuropharmacology Pharmacogenetics and Inflammatory Bowel Disease
Current Pharmacogenomics and Personalized Medicine Role of Defensins and Cathelicidin LL37 in Auto-Immune and Auto- Inflammatory Diseases
Current Pharmaceutical Biotechnology Natural Product-Derived Small Molecule Activators of Hypoxia-Inducible Factor-1 (HIF-1)
Current Pharmaceutical Design Structure Guided Design of Biotin Protein Ligase Inhibitors for Antibiotic Discovery
Current Topics in Medicinal Chemistry Guanidinyl and Amide Conjugated Benzothiazoles as Potential Anti- Tubercular Agent and their Cytotoxicity Study
Anti-Infective Agents Elucidating Unusual Modes of Action and Resistance of Antibacterial Peptides
Current Topics in Medicinal Chemistry Morbidity and Mortality Associated with Pharmacotherapy. Evolution and Current Concept of Drug-Related Problems
Current Pharmaceutical Design Chorismate Synthase: An Attractive Target For Drug Development Against Orphan Diseases
Current Drug Targets The β and γ Classes of Carbonic Anhydrase
Current Pharmaceutical Design