Synthesis, Characterization and Antifungal Activity of N-Methyl Chitosan and Its Application on the Gauze

Title:Synthesis, Characterization and Antifungal Activity of N-Methyl Chitosan and Its Application on the Gauze

Volume: 14 Issue: 4

Author(s): Mardiyah Kurniasih*, Ratna S. Dewi, Purwati Purwati, Dadan Hermawan and Hassan Y. Aboul-Enein*

Affiliation:

• Department of Chemistry, Faculty of Mathematics and Natural Sciences, Jenderal Soedirman University, Jl. Dr. Soeparno, Purwokerto 53123,Indonesia

• Department of Pharmaceutical and Medicinal Chemistry, Pharmaceutical and Drug Industries, Research Division, National Research Centre, Dokki, Giza 12622,Egypt

Keywords: Chitosan, N-methyl chitosan, gauzed, antifungal activity, C. albicans, respiratory tract.

Abstract: Background: Chitosan is a natural polymer which has limited solubility. Chitosan is capable of being modified using multiple alternatives for chitosan chemical modifications that provide different desirable properties.

Method: N-methyl chitosan was successfully prepared and characterized. N-methyl chitosan was characterized by the FTIR, XRD, SEM, TEM, solubility, water content, and specific surface area. Antifungal activity against Candida albicans of N-methyl chitosan and gauze coated N-methyl Chitosan were also determined in this study. The antifungal activity tests were performed using agar diffusion and broth microdilution methods. Gauze coating with N-methyl chitosan was performed with a pad-dry-cure method. The SEM photograph of N-methyl chitosan clearly showed the rugged nature of particles. The TEM image shows that N-methyl chitosan produced from synthesis possessed round shape, with an average diameter of 50 nm.

Results: The results of solubility in water, water content, and specific surface of N-methyl chitosan were 2.04 mg/mL, 18.12%, and 0.995 m/g, respectively.

Conclusion: N-methyl chitosan exhibited antifungal activity against C. albicans with MIC and MFC 0.75% and 1%, respectively. The maximum clear zone produced by gauze coated N-methyl chitosan occurred at 1% concentration of N-methyl chitosan, 60 seconds of dipping time, and 10 times of dipping frequency.

Cite this article as:

Kurniasih Mardiyah*, Dewi S. Ratna , Purwati Purwati , Hermawan Dadan and Aboul-Enein Y. Hassan *, Synthesis, Characterization and Antifungal Activity of N-Methyl Chitosan and Its Application on the Gauze, Current Bioactive Compounds 2018; 14 (4) . https://dx.doi.org/10.2174/1573407213666170420171005