Mini-Review of the Phosphate Center Activation Strategy in Nucleoside Triphosphate Preparation

Title:Mini-Review of the Phosphate Center Activation Strategy in Nucleoside Triphosphate Preparation

Volume: 14 Issue: 6

Author(s): Tomasz P. Kaczynski and Marcin K. Chmielewski*

Affiliation:

• Institute of Bioorganic Chemistry, Polish Academy of Science, Noskowskiego 12-14, 61-704, Poznan,Poland

Keywords: Nucleoside triphosphates, phosphate center activation, phosphitylation, triphosphitylation, phosphoramidite, pyrophosphate.

Abstract: Background: Nucleoside triphosphates (NTPs) synthesis has recently been an area of searching for new methods and possible applications in biochemistry and medicine. However, we still lack a universal tool to synthesize NTPs easily and efficiently. For years, the most popular method has been the “one-pot, three step” conception by Ludwig with its subsequent improvements which used Yoshikawa's et al. monophosphorylation method. Further investigation of these reactions has proven many disadvantages: low yields, many byproducts and problems with purification. But they were a starting point for a whole branch of NTPs synthetic methods.

Method: Analysis of this evolution process demonstrates some correctness: using a pyrophosphate as a nucleophile and mechanism involving a cyclic intermediates at some stage. What is changing is the idea of phosphorus center activation and the resulting improvements and limitations.

Conclusion: In this paper we would like to discuss some new achievements in NTPs synthesis considering their role in changing the strategy of phosphorus center activation depending on the needs

Cite this article as:

Kaczynski P. Tomasz and Chmielewski K. Marcin *, Mini-Review of the Phosphate Center Activation Strategy in Nucleoside Triphosphate Preparation, Mini-Reviews in Organic Chemistry 2017; 14 (6) . https://dx.doi.org/10.2174/1570193X14666170420113635