Background: The strong association between HLA-B27 and
spondyloarthritis (SpA) has demonstrated that typing the HLA-B27 antigen is
a crucial step in diagnosis and aids in defining the progression and
severity of disease.
Objective: To describe the frequency of HLA-B27 in Colombian individuals
with clinical manifestations associated with SpA.
Materials and Methodology: We retrospectively analyzed 4109 HLA-B27 typing
requests to the Hospital Militar Central and the Instituto de Referencia
Andino from Colombian individuals with clinical signs suggestive of SpA
between 2009 and 2012. We used basic digital cytometry followed by
Polymerase Chain Reaction with sequence specific primers when confirmation
was needed. We determined the frequency of HLA-B27 in the population and
levels of association of HLA-B27 with SpA.
Results: Our population included 1585 men (36.8%) and 2524 women (61.4%).
The predominant age range was between 19 and 45 years (49.9%). The majority
(95.4%) of the study population came from the Andean region and eastern
plains. Only a small fraction (12.1%) of the 497 subjects were HLA-B27
positive, among whom 56.9% were male, and 62.5% were between 19 and 45 years
old (p <0.0001). Women constituted 43.1% of the sample, with 7.8% under 18
years old and 37.4% above 45 years old. The most frequent clinical
manifestations were peripheral: synovitis, polyarthralgias, musculoskeletal
pain and heel pain.
Conclusion: The frequency of the HLA-B27 allele in individuals with clinical
signs suggestive of SpA was low, in accordance with the lower prevalence
found in Colombian patients diagnosed with SpA compared to American and