Knockdown of C-C Chemokine Receptor 5 (CCR5) is Protective Against Cerebral Ischemia and Reperfusion Injury

Title:Knockdown of C-C Chemokine Receptor 5 (CCR5) is Protective Against Cerebral Ischemia and Reperfusion Injury

Volume: 14 Issue: 2

Author(s): Edna Constanza Gómez Victoria, Eliana Cristina de Brito Toscano, Ana Clara de Sousa Cardoso, Daniele Gonçalves da Silva, Aline Silva de Miranda, Lucíola da Silva Barcelos, Michelle Adriane Sugimoto, Lirlândia Pires Sousa, Isabel Vieira de Assis Lima, Antônio Carlos Pinheiro de Oliveira, Fátima Brant , Fabiana Simao Machado, Mauro Martins Teixeira, Antônio Lúcio Teixeira and Milene Alvarenga Rachid*

Affiliation:

• Universidade Federal de Minas Gerais, Instituto de Ciencias Biologicas. Departamento de Patologia Geral. Laboratorio de Apoptose. Campus Pampulha, Av. Antonio Carlos 6.627 - Belo Horizonte, Minas Gerais,Brazil

Keywords: Brain, ischemia, reperfusion, chemokines, CCR5, BDNF, mice.

Abstract: Background: Stroke is the second leading cause of death and a major cause of disability of adults worldwide. Inflammatory processes are known to contribute to the pathophysiology of cerebral ischemia, especially following reperfusion. Chemokines and their receptors are involved in migration of leukocytes and have been implicated in the pathogenesis of ischemic stroke.

Objective: In the present study, we investigated the effects of C-C chemokine receptor type 5 (CCR5) deficiency on neurological outcome, brain damage and expression of pro-inflammatory chemokines: chemokine (C-X-C motif) ligand 1 (CXCL1), chemokine (CC motif) ligand 3 (CCL3) and chemokine (C-C motif) ligand 5 (CCL5), and the brain-derived neurotrophic factor (BDNF).

Methods: Adult male C57BL/6 (wild-type) (WT) and CCR5 deficient mice were subjected to transient cerebral ischemia induced by 25 min of bilateral common carotid artery occlusion (BCCAO) followed by 24 hours of reperfusion. Mice were divided into four groups: WT sham group, which underwent sham operation; WT ischemic group, which was subjected to transient bilateral common carotid artery occlusion, CCR5-/- sham group, which underwent sham operation, and CCR5-/- ischemic group, which was subjected to transient BCCAO.

Results: In CCR5 deficiency, we observed a significant improvement in the neurological deficits associated with decreased brain infarcted area as evaluated by triphenyltetrazolium chloride (TTC). Moreover, CCR5 deficiency revealed decreased percentage of necrotic cavities areas and frequency of ischemic neurons by histometric analysis. In addition, CCR5-/- ischemic animals showed lower brain levels of the chemokine CXCL1 and higher levels of BDNF by ELISA, compared with WT BCCAo mice.

Conclusion: Taken together, our results suggest a potential neuroprotection in the absence of CCR5 receptor during global brain ischemia and reperfusion injury.

Cite this article as:

Victoria Constanza Gómez Edna , de Brito Toscano Cristina Eliana, de Sousa Cardoso Clara Ana, da Silva Gonçalves Daniele, de Miranda Silva Aline, da Silva Barcelos Lucíola, Sugimoto Adriane Michelle , Sousa Pires Lirlândia, de Assis Lima Vieira Isabel , de Oliveira Carlos Pinheiro Antônio , Brant Fátima , Machado Simao Fabiana, Teixeira Martins Mauro , Teixeira Lúcio Antônio and Rachid Alvarenga Milene*, Knockdown of C-C Chemokine Receptor 5 (CCR5) is Protective Against Cerebral Ischemia and Reperfusion Injury, Current Neurovascular Research 2017; 14 (2) . https://dx.doi.org/10.2174/1567202614666170313113056

DOI https://dx.doi.org/10.2174/1567202614666170313113056	Print ISSN 1567-2026
Publisher Name Bentham Science Publisher	Online ISSN 1875-5739