Abstract
Background: Tumour microenvironment is recognized as a major determinant of intrinsic resistance to anticancer therapies. In solid tumour types, such as breast cancer, lung cancer and pancreatic cancer, stromal components provide a fibrotic niche, which promotes stemness, EMT, chemo- and radioresistance of tumour. However, this microenvironment is not recapitulated in the conventional cell monoculture or xenografts, hence these in vitro and in vivo preclinical models are unlikely to be predictive of clinical response; which might attribute to the poor predictively of these preclinical drug-screening models.
Conclusion: In this review, we summarized recently developed co-culture platforms in various tumour types that incorporate different stromal cell types and/or extracellular matrix (ECM), in the context of investigating potential mechanisms of stroma-mediated chemoresistance and evaluating novel agents and combinations. Some of these platforms will have great utility in the assessment of novel drug combinations and mechanistic understanding of the tumor-stroma interactions.Keywords: Tumour microenvironment, drug development, tumour-stroma crosstalk, co-culture, cancer, anti-cancer drug.
Combinatorial Chemistry & High Throughput Screening
Title:Development of In Vitro Co-Culture Model in Anti-Cancer Drug Development Cascade
Volume: 20 Issue: 5
Author(s): Ruiling Xu*Frances M. Richards
Affiliation:
- West China School of Medicine, Sichuan University, Chengdu, 610041,China
Keywords: Tumour microenvironment, drug development, tumour-stroma crosstalk, co-culture, cancer, anti-cancer drug.
Abstract: Background: Tumour microenvironment is recognized as a major determinant of intrinsic resistance to anticancer therapies. In solid tumour types, such as breast cancer, lung cancer and pancreatic cancer, stromal components provide a fibrotic niche, which promotes stemness, EMT, chemo- and radioresistance of tumour. However, this microenvironment is not recapitulated in the conventional cell monoculture or xenografts, hence these in vitro and in vivo preclinical models are unlikely to be predictive of clinical response; which might attribute to the poor predictively of these preclinical drug-screening models.
Conclusion: In this review, we summarized recently developed co-culture platforms in various tumour types that incorporate different stromal cell types and/or extracellular matrix (ECM), in the context of investigating potential mechanisms of stroma-mediated chemoresistance and evaluating novel agents and combinations. Some of these platforms will have great utility in the assessment of novel drug combinations and mechanistic understanding of the tumor-stroma interactions.Export Options
About this article
Cite this article as:
Xu Ruiling*, Richards M. Frances , Development of In Vitro Co-Culture Model in Anti-Cancer Drug Development Cascade, Combinatorial Chemistry & High Throughput Screening 2017; 20 (5) . https://dx.doi.org/10.2174/1386207320666170202093538
DOI https://dx.doi.org/10.2174/1386207320666170202093538 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
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