Abstract
Peptide drugs have received more attention as potential next-generation drugs or drug leads. Such attention is primarily due to the higher target selectivities and efficiencies of peptide drugs. Moreover, peptide drugs typically have lower accumulation rates and toxicities. Although peptide drugs have advanced significantly in the 21st century, two bottlenecks (disadvantages) primarily hinder peptide drug research and development compared to small molecule drugs. One bottleneck is the identification of a valuable peptide in a complicated pool of crude products; the other bottleneck is the ability to prepare sufficient amounts of peptides for preclinical or clinical trials. This review discusses important methodologies for addressing these two bottlenecks.
Keywords: Extracellular expression, intracellular expression, in vitro screening, in vivo screening, methodology, peptide.
Current Proteomics
Title:The Development of High-throughput Identification and Heterologous Expression of Valuable Peptides/Proteins
Volume: 14 Issue: 1
Author(s): Chang-Jun Liu, Lei Wu, Er Meng and Dong-Yi Zhang
Affiliation:
Keywords: Extracellular expression, intracellular expression, in vitro screening, in vivo screening, methodology, peptide.
Abstract: Peptide drugs have received more attention as potential next-generation drugs or drug leads. Such attention is primarily due to the higher target selectivities and efficiencies of peptide drugs. Moreover, peptide drugs typically have lower accumulation rates and toxicities. Although peptide drugs have advanced significantly in the 21st century, two bottlenecks (disadvantages) primarily hinder peptide drug research and development compared to small molecule drugs. One bottleneck is the identification of a valuable peptide in a complicated pool of crude products; the other bottleneck is the ability to prepare sufficient amounts of peptides for preclinical or clinical trials. This review discusses important methodologies for addressing these two bottlenecks.
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Cite this article as:
Liu Chang-Jun, Wu Lei, Meng Er and Zhang Dong-Yi, The Development of High-throughput Identification and Heterologous Expression of Valuable Peptides/Proteins, Current Proteomics 2017; 14 (1) . https://dx.doi.org/10.2174/1570164614666161207162711
DOI https://dx.doi.org/10.2174/1570164614666161207162711 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
Call for Papers in Thematic Issues
Mass spectrometry data acquisition and analysis for proteomics
The Thematic Issue on "Mass spectrometry data acquisition and analysis for proteomics" aims to explore the latest advancements and challenges in the field of proteomics through the lens of mass spectrometry. Proteomics, the large-scale study of proteins and their functions, plays a crucial role in understanding various biological processes and ...read more
Peptides: State-of-Art and Commercialisation Hurdles
The Editors of the Current Proteomics (CP) journal are highly privileged to welcome scientists to submit their scientific research and review articles to be considered for publication in the upcoming thematic issue. The topics should cover various aspects of peptides in regard to their synthetic methodologies, formulation approaches, pharmacological challenges, ...read more
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