Validated Ultra Performance Liquid Chromatography-Tandem Mass Spectrometric Method for Determination of Betamethasone or Dexamethasone in Pharmaceuticals

Title:Validated Ultra Performance Liquid Chromatography-Tandem Mass Spectrometric Method for Determination of Betamethasone or Dexamethasone in Pharmaceuticals

Volume: 14 Issue: 1

Author(s): Mohammad Jamal Amer Shammout and Nursabah Elif Basci*

Affiliation:

• Analytical Chemistry Department, Faculty of Pharmacy, Hacettepe University, Ankara,Turkey

Keywords: Dexamethasone, betamethasone, UPLC-MS/MS, tablet, ampoule, drops.

Abstract: Introduction: Chromatographic methods for determination of betamethasone or dexamethasone in their pharmaceutical preparations by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry were developed.

Method: Analytes were eluted from Acquity BEH C18 (50 mm×2.1 mm, 1.7 µm) column using stepwise gradient with mobile phase consisted of acetonitrile and 0.1% (v/v) acetic acid in water. Quantitation of dexamethasone and betamethasone was performed using 451>361 m/z on MRM mode.

Results: Methods were linear over the concentration range from 10 to 1500 ng mL -1 for both analyte. Limits of detection for BTM or DXM were 1 ng mL -1. The highest relative error and relative standard deviation values were not over 2%.

Conclusion: Presented methods were specific, rugged and robust, successfully applied to the analysis of their pharmaceutical preparations.

Cite this article as:

Amer Shammout Jamal Mohammad and Basci Elif Nursabah*, Validated Ultra Performance Liquid Chromatography-Tandem Mass Spectrometric Method for Determination of Betamethasone or Dexamethasone in Pharmaceuticals, Current Pharmaceutical Analysis 2018; 14 (1) . https://dx.doi.org/10.2174/1573412912666161018145227