Introduction: Rabies, the most fatal of all infectious diseases, remains a major
public health problem in developing countries. The presence of neutralizing and nonneutralizing
antiviral antibodies in the serum and cerebrospinal fluid suggests that immune
responses are not only responsible for virus clearance but also contribute to pathogenesis
revealing the possibility of autoimmunity causing the death. In spite of the developments of
costly vaccines, there is no cure for this infection as the drugs etc. do not cross the blood
brain barrier to treat the encephalitis in the brain and cerebrospinal and other neural systems.
The neuroinvasive functionalized silver nanoparticles with whole viral antigen/ antibodies
can neutralize the autoantibody/antigens restoring the neurological dysfunction arise
due to autoimmunity.
Methods: Commercially available veterinary rabies vaccines were used for functionalization
of nanoparticles for all animal studies and for human use commercial human vaccines
were used. The immuno-dominant polypeptides for neutralization and bio distribution studies
were carried out. The field trial was carried out to rapid dogs, cattle and one human.
Results: The present invention - The nanoformulation elicits an optimal immuno protective
response against rabies antibody /virus by neutralizing auto immune antibodies/ virus / antigens
of rabies infection. The distribution pattern in various tissues and neutralization with
these functionalized nanopreparation strongly recommend the use for prophylactic,
therapeutic and diagnostics. This novel innovative strategy cures rabies infection which
causes 100% mortality within 2 hr to 12 hrs as proved by the field trial report.
Discussion: The low cost nanofomulations, in single minimum dosage, with high stability
and without cold chain storage could neutralize all the autoantibody/antigens restoring the
neurological dysfunction. This novel innovative strategy signifies the birth of a new era in
therapeutics for all microbial infections leading to autoimmune diseases.