Identification of Antioxidative Components in ChuanKeZhi Injection and Discussion on Its Quality Control Method

Title:Identification of Antioxidative Components in ChuanKeZhi Injection and Discussion on Its Quality Control Method

Volume: 13 Issue: 5

Author(s): Shuai Sun*, Hailing Liu, Yijuan Xu, Longgang Guo, Shunjun Xu, Liu Yang and Peishan Xie

Affiliation:

• Guangzhou ImVin Pharmaceutical Co., Ltd., No. 2 Fangcaodian road, Science City, Guangzhou,China

Keywords: Antioxidant, flow injection, ChuanKeZhi, asthma, reactive oxygen species, quality control

Abstract: Intoduction: ChuanKeZhi, an intramuscular Traditional Chinese Medicine injection, has been used to treat asthma for more than twenty years in China and good clinical results have been received. However, the material basis remains to be elucidated.

Materials and Method: Aiming at disclosing the antioxidants in ChuanKeZhi, an online identification system adopting high performance liquid chromatography tandem chemiluminescence detector was carried out. Two lignanosides named (-)-olivil-4’-O-glucopyranoside and (+)-cycloolivil were proved to be mainly responsible for scavenging hydrogen peroxide, and their structures were confirmed by NMR data. Chemiluminescence inhibition was calculated as the ratio of the maximum intensity in the presence of injection to that in its absence, and logarithmic values of inhibition ratio showed a good linear correlation with the concentrations of cycloolivil in the range of 4.950-158.3 μg/mL (R²=0.9917). Principal component analysis disclosed that contents of these two compounds explained mostly the variance of total antioxidative activities of different batches.

Result: This work revealed the components contributed to antioxidative activity of ChuanKeZhi injection and might provide some information for new drug development and better quality control method.

Cite this article as:

Sun Shuai*, Liu Hailing, Xu Yijuan, Guo Longgang, Xu Shunjun, Yang Liu and Xie Peishan, Identification of Antioxidative Components in ChuanKeZhi Injection and Discussion on Its Quality Control Method, Current Pharmaceutical Analysis 2017; 13 (5) . https://dx.doi.org/10.2174/1573412912666160919115110

DOI https://dx.doi.org/10.2174/1573412912666160919115110	Print ISSN 1573-4129
Publisher Name Bentham Science Publisher	Online ISSN 1875-676X